com Liquid Chromatography AP Chemistry Laboratory # 18 Publication No. 10535A Catalog No. AP9093 Introduction In this experiment‚ liquid chromatography is used to separate the substances that are present in grape-flavored Kool-Aid®. First‚ the dyes responsible for the purple color‚ FD&C Blue #1 and Red #40 are separated. Then‚ in a second experiment‚ the other components of Kool-Aid®‚ the flavorings and citric acid‚ are separated as well. Concepts • Resolution • Liquid chromatography • Selectivity
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9.1 Orbitals and Theories of Chemical Bonding 1. Which one of the statements concerning valence bond (VB) and molecular orbital (MO) bond theories is correct? a) MO theory predicts that electrons are localized between pairs of atoms. b) In VB theory‚ bonding electrons are delocalized over the molecule. c) MO theory accurately describes bonding in O2 and NO‚ VB theory does not. d) VB theory can describe molecular bonding in excited states. e) MO theory is used to accurately predict
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Liquid Chromatography – Laboratory #18 Introduction: We are using liquid chromatography to separate the colored substances in grape-flavored drinks. We separate the component dyes‚ and then we separate the flavorings and citric acids. Background: Chromatography is a process that is used to separate a substance into its component parts. The separation occurs between the stationary and moving phase of the lab. The moving phase consists of a fluid and the stationary phase consists of a solid
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Performance Liquid Chromatography (HPLC) Instructor: Dr. Hüseyin BOZKURT High Performance Liquid Chromatography (HPLC) is one mode of chromatography‚ the most widely used analytical technique. Chromatographic processes can be defined as separation techniques involving mass-transfer between stationary and mobile phases. Present day liquid chromatography that generally utilizes very small packing particles and a relatively high pressure is referred to as high performance liquid chromatography (HPLC)
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Graded Assignment Honors Project 1: Liquid Breathing Write the final draft of your project. Be sure to follow these requirements and recommendations when completing your draft: • Open a new Microsoft Word document. Type your name‚ your teacher’s name‚ your school name‚ and the date at the top of your document. To help your teacher know from whom the project came‚ save the file as: CHEM_304B_10_01_Honors_Project_1_Final_Draft_FirstInitial_LastName.doc
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Separation techniques LIQUID CHROMATOGRAPHY ‘THE ART OF SEPARATION’ CHROMATOGRAPHY – AN INTRODUCTION Chromatography is a technique through which a mixture of chemical components are separated‚ identified and determined accurately. This technique while provides a way for analytical separations‚ also useful for preparative techniques by which pure compounds can be obtained. Detector Signal Blue Compound Sample Injection + Mobile Phase Retention Time Red Compound It is i defined d fi d as a
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of solvent extraction‚ also known as liquid-liquid extraction‚ a method of separating two materials with different physical and/or chemical properties. Two methods of evaporation will be used to finally determine the concentration of impurity- one using an explosion-proof hot-plate‚ and one using a rotovap. III. Theory Fig. 1a+1b. Figure 1a shows how water and oil separate in the separatory funnel‚ while figure 1b shows the actual apparatus. Liquid-Liquid extraction is a versatile and dependable
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Experiment 4 – Liquid Phase Chromatography I. Objectives This experiment’s goal is to explore one-dimensional and two-dimensional paper chromatography. II. Schematic Diagram of the Procedure PAPER CHROMATOGRAPHY Wash leaves‚ cut them into smaller pieces; in a mortar macerate them in circular motion Add 8mL ethyl alcohol to extract pigments‚ continue macerating until finely grounded Transfer extract to evaporating dish‚ allow to conc‚ don’t let extract to dry out Concentration
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Chemistry Project on Evaporation of Liquid Acknowledgement I would like to show my gratitude to my Chemistry Teachers Ms Seema More without whose support and encouragement this project could never have have taken the present shape . I would also like to thank my lab incharge Mr. Kakde For helping Me out During The Practical Part Of This Project. Aim Acknowledgment I am are greatly thankful for the cooperation and help from the Community Science Center (CSC) authorities and the Chemistry lab
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1. A class that I believe was most particularly significant to me is AP Chemistry. Prior to enrolling in the class‚ I was mostly breezing through school without having to put in a considerable amount of time for homework and studying purposes. A few weeks into the course however‚ I had realized that this class was not going to be even close to the ones I have taken before in terms of the rigor and depth of understanding required to succeed. Now that I have finished the course‚ albeit with a B first
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