Optimization of Gas Chromatography
CHM 510 LABORATORY REPORT
Experiment 1: Gas Chromatography (GC): Optimization of Flow Rate and Column Temperature
Name: AFIQ B. ANWAR
Student No.: 2012621072 (AS2253A)
Date of experiment:
Date of report submission:
Lecturer’s Name: PN. HALIZA
Gas Chromatography (GC): Optimization of Flow Rate and Column Temperature
INTRODUCTION
The main purpose of the experiment is to investigate the effects of column temperature and flow rate on the separation of methyl esters compounds. On top of that, the experiment is conducted to explore the gas chromatography, which is include the concepts of retention time and resolution using a mixture of methyl esters: methyl linolate, methyl laurate, methyl myristate, and methylpalmitate and methyl stearate. In this experiment, the isothermal and temperature programming are required in order to get an optimize parameter. The efficient separation of compounds in GC is dependent on the compounds travelling through the column at different rates. There are several factors at which a compound travels through a particular GC systems depends on that include column temperature, carrier gas flow rate, length of the column and volatility of a compound. In this context, the increase in column temperature will speeds up the elution time of a compound mixture. Moreover, as the carrier gas flow rate is increase, the speeds at which all compounds move through the column will also increase. All these factors are related to the optimization of the gas chromatography in order to have a good resolution.
Gas chromatography (GC) is a widely used technique in which the mobile phase is a carrier gas, usually helium or nitrogen and the stationary phase is a microscopic layer of liquid or polymer on an inert solid support inside glass or metal tubing. In order for separation to occur, the stationary phase must have an affinity for the analytes in the sample mixture. The mobile phase is inert and does not interact chemically
Experiment 1: Gas Chromatography (GC): Optimization of Flow Rate and Column Temperature
Name: AFIQ B. ANWAR
Student No.: 2012621072 (AS2253A)
Date of experiment:
Date of report submission:
Lecturer’s Name: PN. HALIZA
Gas Chromatography (GC): Optimization of Flow Rate and Column Temperature
INTRODUCTION
The main purpose of the experiment is to investigate the effects of column temperature and flow rate on the separation of methyl esters compounds. On top of that, the experiment is conducted to explore the gas chromatography, which is include the concepts of retention time and resolution using a mixture of methyl esters: methyl linolate, methyl laurate, methyl myristate, and methylpalmitate and methyl stearate. In this experiment, the isothermal and temperature programming are required in order to get an optimize parameter. The efficient separation of compounds in GC is dependent on the compounds travelling through the column at different rates. There are several factors at which a compound travels through a particular GC systems depends on that include column temperature, carrier gas flow rate, length of the column and volatility of a compound. In this context, the increase in column temperature will speeds up the elution time of a compound mixture. Moreover, as the carrier gas flow rate is increase, the speeds at which all compounds move through the column will also increase. All these factors are related to the optimization of the gas chromatography in order to have a good resolution.
Gas chromatography (GC) is a widely used technique in which the mobile phase is a carrier gas, usually helium or nitrogen and the stationary phase is a microscopic layer of liquid or polymer on an inert solid support inside glass or metal tubing. In order for separation to occur, the stationary phase must have an affinity for the analytes in the sample mixture. The mobile phase is inert and does not interact chemically
References: 1. What is gas chromatography? Agilent technologies life sciences/chemical analysis online. July 2009. 2. Holler, Skoog, Crouch, Principle of Instrumental Analysis, Thomson Brooks/Cole Publishers, 6th Edition, 791-792. 3. Skoag, West, Holler, Crouch, Fundamental of Analytical Chemistry, Thomson Brooks/Cole Publishers, 8th ed., 2004 4. Chromatography-Introductory Theory retrieved 26th December 2012 from http://teaching.shu.ac.uk/hwb/chemistry/tutorials/chrom/chrom1.htm