Natalie Salaverria 01/21/11 LAB EXERCISE I After the first exercise : Simulating Simple diffusion Which Materials diffused from the left beaker to the right beaker? NaCl ‚ Urea ‚ Glucose Which did not ? Albumin Why ? Albumin’s Composition or charge was too great to diffuse through the membrane. After the 2nd activity : Simulating Dialysis What happens to the urea concentration in the left beaker (the Patient)? It diffused to the right beaker Why does this occur? The excess
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was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction? a. Urea was unable to diffuse through the 20MWCO membrane because urea is a big molecule and the 20MWCO membrane pore size was too small for Urea. My predication was accurate‚ as I predicted that Urea would not be able to diffuse through the 20MWCO membrane 3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results
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increase in blood pressure Experiment Data: Run Number 1 1 1 1 2 2 2 2 3 3 3 3 4 4 4 4 Solute Na+ ClUrea Glucose Powdered Charcoal Na+ ClUrea Glucose Powdered Charcoal Na+ ClUrea Glucose Powdered Charcoal Na+ ClUrea Glucose Powdered Charcoal MWCO 20 20 20 20 50 50 50 50 200 200 200 200 200 200 200 200 Pressure 50 50 50 50 50 50 50 50 50 50 50 50 100 100 100 100 Filter Rate 1.00 1.00 1.00 1.00 2.50 2.50 2.50 2.50 10.00 10.00 10.00 10.00 20.00 20.00 20.00 20.00 Residue present present present present
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FACILITATED IS MOVEMENT WITH THE HELP OF CARRIER PROTEINS 3. What is the main difference between facilitated diffusion and active transport? ACTIVE TRANSPORT USES ATP 4. In the “Simple Diffusion” experiment‚ which solute(s) passed through the MWCO 20 membrane? NONE Why? SOLUTES MASSES ARE TO BIG 5. List three examples of passive transport mechanisms. DIFFUSION FILTRATION OSMOSIS 6. Describe the relationship of solute concentration to solvent concentration in osmosis. CONCENTRATION
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During which phase of the cell cycle does DNA duplication‚ or replication‚ take place? – interphase When a double helix of DNA is replicated‚ two complete helices are formed. Together‚ these helices are called sister __________. – chromatids During which phase of mitosis do sister chromatids line up at the center of the cell? – metaphase During which phase of mitosis do the sister chromatids move apart? – Anaphase During which phase of mitosis do nuclear envelopes and the nucleoli reappear?
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imple Diffusion 1. The following refer to Activity 1: Simulating Dialysis (Simple Diffusion). Which solute(s) were able to pass through the 20 MWCO membrane? According to your results‚ which solute had the highest molecular weight? ______________________________________ Which solute displayed the highest rate of diffusion through the 200 MWCO membrane? __________________________ Using the data from Chart 1‚ explain the relationship between the rate of diffusion and the size of the solute. Facilitated
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change in osmotic pressure across a membrane? The solutes can diffuse through the pores and the concentration of solutes is the same on both sides of the membrane. With the experimental conditions set at 10 mM glucose and 9 mM albumin‚ and the 200 MWCO membrane in place‚ which of the following is true? The net movement of water is toward the albumin. Which of the following can be true of both active transport and facilitated diffusion? Lipid-insoluble solutes are transported across the membrane
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responsible for haze formation and browning during storage‚ could be selectively removed by combined enzyme-ultrafiltration process. The pomegranate juice was treated with Fomes fomentarius laccase at various enzyme concentrations (0.5–5 U/ mL)‚ temperatures (20–50C) and times (30–300 min). The effect of these enzymatic treatments on total phenol content and clarity of the juice were evaluated using a central composite design. The optimum treatment conditions were: enzyme concentration 5 U/mL; time 300 min;
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Chapter one Introduction In industrial processing water is used in numerous applications requiring likewise different qualities of water. Examples of different use are cooling water‚ water for rinsing and chemical production‚ boiler feed water‚ purified water‚ water for injection‚ just to mention a few of those. On a chemical site or within a factory commonly a central water production unit is providing the basic amount of water in several qualities. These water supply units are using more and
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UPW System Introduction Purpose of this training Introduction of units • • • Theory Application/Function in DI System Operation After this training: • • • Know the theory of main units Understand the function of units in DI system Understand the possible causes of abnormal performance Daily Operation To take care of the system • • • • To watch To listen To smell To feel Why??? • • • • To find out problem on time‚ minimize loss To prevent
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