through? |D | |50 MWCO membrane | | |100 MWCO membrane | | |200 MWCO membrane
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Materials and methods: * 20 mwco dialysis membrane * 50 mwco dialysis membrane * 100 mwco dialysis membrane * 200 mwco dialysis membrane * Membrane holder * NaCl concentration * Urea * Albumin * Glucose * Deionized water * Beakers The experiment wasn’t done in an actual lab‚ but rather using a computer simulation in order to achieve precision and save time. Results: At 20 MWCO ‚NaCl (9.00 mM) no diffusion occurred . At 50 MWCO‚ NaCl(9.00 mM) diffusion
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Dialysis (Simple Diffusion) Answers 1. The molecular weight of urea is approximately 60. Which of the membranes can it pass through? a. 50 MWCO membrane b. 100 MWCO membrane c. 200 MWCO membrane d. Both the 100 and 200 MWCO membranes D. Both the 100 and 200 MWCO membranes 2. True or False: A solution containing glucose will diffuse faster through a 200 MWCO membrane if it is heated. True 3. The molecular weight of carbon C is 12; the molecular weight of hydrogen (H) is 1; the molecular weight
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Lab Report #1 Introduction A cell’s plasma membrane is known to be selectively permeable. This implies that the membrane is selective on what substances can pass in and out of the cell. There are two methods of transport that occur through the plasma membrane. One method of transport is called active process which uses ATP energy to transport substances through the membrane. The other method is called passive process which does not require the use of ATP energy. During passive processes
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mosis Did you observe any pressure changes during this experiment? If so‚ in which beaker(s)‚ and with which membranes? In the L beaker with the 20 MWCO Membrane‚ the pressure went up. Why? Because no diffusion took place. Did the Na?/Cl? diffuse from the left beaker to the right beaker? If so‚ with which membrane(s)? Yes‚ 50‚ 100‚ and 200 MWCO Why? Because diffusion took place. Explain the relationship between solute concentration and osmotic pressure. They are directly proportional‚ if the
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11M01_ZAO2177_01_SE_CH01.QXD 3/4/11 1:36 AM Page 1 E X E R C I S E 1 Cell Transport Mechanisms and Permeability P R E - L A B Q U I Z 1. Circle the correct term. A passive process‚ diffusion / osmosis is the movement of solute molecules from an area of greater concentration to an area of lesser concentration. 2. A solution surrounding a cell is hypertonic if: a. it contains fewer nonpenetrating solute particles than the interior of the cell. b. it contains
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to diffuse through 20 MWCO? How well did the results compare with your predictions? The urea was not able to diffuse through the 20 MWCO because the membrane pores are too small and the urea molecules are large. The molecular weight of Urea is 60.07 g/mol which is too large thus the molecules were not able to pass through the pores of the 20 MWCO. The results that I obtained from the experiment agreed with these predictions because urea was no able to diffuse through the 20 MWCO. 3. Describe
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1. What is the molecular weight of Na+? 22.99 2. What is the molecular weight of Cl-? 35.45 3. Which MWCO dialysis membranes allowed both of these ions through?50‚ 100 and 200 4. Which materials diffused from the left beaker to the right beaker Urea‚ NaCl and glucose diffused 5. Which did not? Why? Albumin‚ because it is too large to diffuse Activity 2: Simulating Dialysis 1. What happens to the urea concentration in the left beaker (the patient)? Its concentration gradient
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Hypothesis – Activity 2: Simulating Facilitated Diffusion “I hypothesize that a higher concentration gradient would result in a higher rate of glucose transport” Materials and Methods: Activity #1 Materials: * 2 Beakers * 4 MWCO Membranes * 20‚ 50‚ 100‚ 200 * 9.00 mM of each solute: * NaCl‚ Urea‚ Albumin‚ Glucose * Deionized water Methods: * The solutes NaCl‚ Urea‚ Albumin‚ and Glucose each in the amount of 9.00 mM were used respectively. * The first step
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concentration. 3. Which of the following dialysis membranes has the largest pore size? You correctly answered: d. 200 MWCO 4. Avogadro’s number is a constant for the number of You correctly answered: b. molecules. 06/17/14 page 1 Experiment Results Predict Question: Predict Question 1: The molecular weight of urea is 60.07. Do you think urea will diffuse through the 20 MWCO membrane? Your answer : c. No‚ not at all. Predict Question 2: Recall that glucose is a monosaccharide‚ albumin
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