affect the rate of diffusion are size and concentration gradient. The molecular size vs the MWCO size of the membrane can either increase‚ decrease‚ or prevent diffusion. The greater the concentration gradient the greater the diffusion rate due to molecules moving from areas of higher concentration to areas of lower concentration. • Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction? My prediction was correct
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variable that affects osmotic pressure is You correctly answered: a. the concentration of nondiffusing solutes. 4. The net movement of water would be into the cell in a Your answer : d. hypnotonic solution. Correct answer: b. hypotonic solution. 01/20/14 page 1 Experiment Results Predict Question: Predict Question 1: What effect do you think increasing the Na+ Cl- concentration will have? Your answer : a. increased osmotic pressure Predict Question 2: What do you think will be the pressure
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same. Experiment Data: Run Number 1 1 1 2 2 2 3 3 3 4 4 4 5 5 5 6 6 6 7 7 7 Solute Na+ ClAlbumin Glucose Na+ ClAlbumin Glucose Na+ ClAlbumin Glucose Na+ ClAlbumin Glucose Na+ ClAlbumin Glucose Na+ ClAlbumin Glucose Na+ ClAlbumin Glucose MWCO 20 20 20 20 20 20 50 50 50 100 100 100 100 100 100 200 200 200 200 200 200 Start Conc. L 5.00 0.00 0.00 10.00 0.00 0.00 10.00 0.00 0.00 0.00 0.00 8.00 0.00 0.00 8.00 0.00 0.00 8.00 0.00 9.00 0.00 02/04/15 Pres. L 170 170 170 340 340 340 0 0 0 136 136 136
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REVIEW SHEET EXERCISE 1 Cell Transport Mechanisms and Permeability 1. Match each of the definitions in Column A with the appropriate term in Column B. |Column A |Column B | |__E__ term used to describe a solution that has a lower |a. diffusion | |concentration of solutes compared to another solution |b. facilitated diffusion
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Dialysis (Simple Diffusion) 1. Describe two variables that affect the rate of diffusion. ___ ___ 2. Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction? ___ 3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your prediction? ___ ___ 4. Put the following in order from smallest to largest molecular weight: glucose‚ sodium chloride
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__________________________________________________________________________________________________ 2. Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction? ____________________________________________________________________________________ 3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your prediction? ___________________________________________________________________
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concentration without the requirement of ATP. The movement of molecules AGAINST the concentration gradient with the use of energy (eg‚ ATP) 4. In the “Simple Diffusion” experiment‚ which solute(s) passed through the MWCO 20 membrane? None of the substances passed through the MWCO 20 membrane. The molecular weight cut
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affect the rate of diffusion. __The size and the concentration. ___ 2. Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction? __ The molecules were to large to go through. My results matched._ 3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your prediction? __The albumin was not able to go through and the glucose did. I predicted
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Activity FOUR There is a direct relationship between the MWCO and the filtration rate; the higher the MWCO‚ the higher the filtration rate. Powdered charcoal is the solute that did not appear in the filtrate when using any MWCO membranes. As pressure increases‚ the filtration rate also increases‚ as long as the MWCO does not decrease. Since we have no control over the MWCO of a living membrane‚ we can increase the rate of filtration by increasing the pressure. The molecular weight
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of the molecule diffusing through the membrane 2. Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction? * Because the pores of the membrane were not large enough * I predicted this correctly. 3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your prediction? I predicted that only glucose would diffuse through
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