Simple Diffusion Activity 1: Simulating Simple diffusion 1. What is the molecular weight of Na+? 22.99 or 23 2. What is the molecular weight of Cl-? 35.45 3. Which MWCO dialysis membranes allowed both of these ions through?50‚100‚ 200 4. Which materials diffused from the left beaker to the right beaker? Urea‚ NaCl and glucose diffused 5. Which did not? Why? Albumin was too large to diffuse into the right beaker. Activity 2: Simulating Dialysis 6. What happens to the urea concentration
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diffusion (eg. steric conformation‚ size‚ polarity‚ amount and strength of hydrogen bonding) 2. Urea was not able to diffuse through the 20 MWCO because the pores of the membrane were too small for the urea to pass through. The molecular weight of urea is 60.06 g/mol‚ over three times greater than the 20 MWCO. 3. Glucose was able to diffuse through the 200 MWCO pore because it was small enough‚ having a molecular weight of 180.2 g/mol. Albumin was unable to pass through because it was far too large
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Permeability Simple Diffusion The following refer to Activity 1: Simulating Dialysis (Simple Diffusion). Which solute(s) were able to pass through the 20 MWCO membrane? NONE 1. According to your results‚ which solute had the highest molecular weight? Albumin 2. Which solute displayed the highest rate of diffusion through the 200 MWCO membrane? Na+Cl 3. Using the data from Chart 1‚ explain the relationship between the rate of diffusion and the size of the solute. The larger the
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concentration. 2. Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction? Your answer: Because Urea was too big to pass through the 20 MWCO membrane 3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your prediction? Your answer: glucose diffused through the 200 MWCO membrane‚ while there was no diffusion of albumin. I predicted correctly
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Lab Report 1: Cell Transport Mechanisms and Permeability Using PhysioEx 8.0 Introduction The purpose of these experiments is to examine the driving force behind the movement of substances across a selective or semiperpeable plasma membrane. Experiment simulations examine substances that move passively through a semipermeable membrane‚ and those that require active transport. Those that move passively through the membrane will do so in these simulations by facilitated
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you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction? The urea was not diffused in the 20 MWCO because the poles of the membrane are smaller than the urea’s molecules. This agreed with my predictions because after all the urea wasn’t able to diffuse in the 20 MWCO substance. 3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your prediction
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there was no pressure change? You correctly answered: c. There is no net movement of water because the solute concentrations are the same. Experiment Data: Run Number 1 2 3 4 5 6 7 Solute Na+ ClNa+ ClNa+ ClGlucose Glucose Glucose Albumin Glucose MWCO 20 20 50 100 100 200 200 200 Start Conc. L 5.00 10.00 10.00 8.00 8.00 8.00 9.00 0.00 Pres. L 170 340 0 136 0 0 153 153 Start Conc. R 0.00 0.00 0.00 0.00 8.00 0.00 0.00 10.00 Pres. R 0 0 0 0 0 0 0 0 Rate 0.0000 0.0000 0.0167 0.0000 0.0000 0.0036 0.0000
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Exercise 1: Cell Transport Mechanisms and Permeability Worksheet. Simple Diffusion Activity 1: Simulating Simple diffusion 1. What is the molecular weight of Na+? 22.99 2. What is the molecular weight of Cl-? 35.45 3. Which MWCO dialysis membranes allowed both of these ions through? 50‚ 100‚ 200 4. Which materials diffused from the left beaker to the right beaker? Na+/Cl-‚ Urea‚ Glucose 5. Which did not? Why? Albumin did not diffuse from the left to the right beaker. The conclusion
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PhysioEx™ Lab: Cell Transport Mechanisms and Permeability Activity 1: Simple Diffusion Chart 1 Dialysis Results (Average Diffusion rate in mM/min) Solute Membrane (MWCO) 20 50 100 200 NaCl (—) 0.0150 0.0150 0.0150 Urea (—) (—) 0.0094 0.0094 Albumin (—) (—) (—) (—) Glucose (—) (—) (—) 0.0040 1) Which solute(s) were able to diffuse into the right beaker from the left? NaCl‚ Urea‚ Glucose 2) Which solute(s) did not diffuse? Albumin 3) If the solution in the left beaker
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was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction? a. I think the urea was not able to diffuse through the 20 MWCO membrane because the urea was too big to pass through. 3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your prediction. a. Between the attempts to diffuse glucose and albumin through the 200 MWCO membrane‚ the results for both were
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