The Antipasmodic Activity of Molave Tree (Vitex Parviflora) Bark Extract in Albino Mice

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The Antipasmodic Activity of Molave Tree (Vitex
parviflora) Bark Extract in
Albino Mice

A Research Paper
Presented to Mrs. Ferrera Oira
In Partial Fulfillment of the Requirement in Research II

Georgette Mae P. Dineros
Jone Zelita C. Orlina
Anna Dominique G. Humbe
IV- Analysts Andromeda

Ramon Teves Pastor Memorial
Dumaguete Science High School
SY 2012-2013

ABSTRACT

Diarrhea is a common condition that affects people of any age and of any gender. It is characterized by frequent bowel movements. This causes dehydration and loss of salts. It may be caused by poor sanitation and contaminated food, milk or water. When diarrhea strikes, the first solution in mind would usually be antidiarrheal drugs. So the researchers thought of an alternative remedy for diarrhea.

In this study, the researchers compared the effects of Molave tree Bark to the effects of antidiarrheal drugs on diarrhea. The preparation of extract was done by crushing 10 mg. of Molave Tree bark mixed with 10 mL distilled water. The bark was strained and the extract was considered our 100% crude extract. The researchers got the 75% and 50% plant extracts by mixing 0.75 mL of 100% plant extract to 0.25 mL distilled water and 0.5 mL of 100% plant extract to 0.5 mL distilled water respectively. For the control, 2 mg. antidiarrheal drugs were mixed with 2 mL distilled water. After starving 12 albino mice for 12 hours, each mouse received 0.2 mL of their respective extract. After one hour, each mouse was given 0.2 mL charcoal meal which is composed of 20 mL castor oil, 10 mL coconut oil and 2 g activated carbon. After another hour, the mice were all sacrificed by chloroform and dissected to get the intestines. The distance traveled by the charcoal was divided by the total length of the intestines and data was subjected to statistical tests.

ACKNOWLEDGEMENT

The researchers would like to thank, first of all, to God Almighty who gave us the strength and determination to pursue our study. We would also like to thank Mrs. Ferrera Oira for the information she shared that contributed to our study, Mr. Ryan Huang for lending us the tools we needed for the experimentation, to Mrs. Joan Dolino for helping with the statistical tests, to Mrs. Melinda Favor for letting us use the library for research purposes, to SU Chemistry Department for providing the necessary chemicals, to Acuña Veterinary Clinic for conducting the dissection, to the loving parents for their financial and moral support, to our ever supportive classmates and schoolmates who gave us the boost of confidence in our study, and to the past researchers who gave us the impetus to continue our study.

INTRODUCTION

Diarrhea is a condition that is not only a pain but also a disturbance to our activities. The bark of the Molave Tree is said to have antipasmodial effects. This folkloric effect stirred the curiosity of the researchers. The researchers wanted to know if Molave Tree Bark is as effective as antidiarrheal drugs on diarrhea.

Background of the Study

The effect of Molave Tree Bark on Diarrhea is already known but not on a scientific basis. The question that the researchers developed is whether Molave Tree Bark is a good alternative to the known medicine of the condition which is antidiarrheal drugs. The researchers chose to have this study to find out if Molave Tree Bark is effective enough that it can be used to treat diarrhea.

Statement of the Problem

Main Problem:
Does Molave tree bark have the same effects on diarrhea as antidiarrheal drugs? Sub Poblems:
At what concentration would it be most effective?
Ho: There is a significant difference in the antipasmodic activity of antidiarrheal drugs and molave tree bark. Ha: There is no significant difference in the antipasmodic activity of antidiarrheal drugs and molave tree bark.

Significance of the Study

Diarrhea has been a common problem for a long...
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