How Environment Can Effect Enzymes Lab Report
Eileen Kirby
Professor Eischen
BIO-151
April 4, 2014
How Environment Can Effect Enzymes
Introduction:
In a chemical reaction there sometimes can be a catalyst present known as an enzyme. An enzyme is a protein that speeds up a chemical reaction by lowering the amount of activation energy required to start the reaction. By lowering the activation energy, more of the substrate is able to participate in the reaction, speeding it up. Enzymes are substrate specific. The substrate is what the enzyme bonds to. That is to say that enzyme A will only react with substrate A, enzyme B with substrate B and so on. The enzyme will bond to the substrate’s active site. When the substrate, or reactant and the enzyme bind the ending result is known as …show more content…
The first part of the experiment was broken into three sections. The first section showed the pH and color changes that an enzyme can create. It was predicted that when potato extract, the enzyme, was added to catechol, the substrate, an enzymatic reaction would occur. The second section of the experiment demonstrated enzyme specificity. It was predicted that potato extract, when hydroquinone was introduced, would not exhibit the characteristics of an enzymatic reaction. The third section of part one focused on whether or not concentration matters. It was predicted that the more enzyme the more intense the reaction. The second part of the experiment concentrated on the effects of pH and temperature on an enzymatic reaction. It was predicted that once a solution containing both a substrate and an enzyme reached a certain temperature or pH content, it would not facilitate a chemical …show more content…
Test Tube A contained 5 drops of distilled water, 10 drops of catechol and 5 drops of potato extract. Test Tube B contained 10 drops of distilled water, 5 drops of catechol, and 10 drops of potato extract. Test Tube C contained 5 drops of potato extract and 20 drops catechol. Test Tube D contained 5 drops of distilled water, 20 drops of potato extract and 5 drops of catechol. Test Tube E contained 10 drops potato extract, 4 drops of catechol and 2 drops of water. After 5 minutes two drops from each tube were tested for their pH and results recorded. Any color changes were also
Professor Eischen
BIO-151
April 4, 2014
How Environment Can Effect Enzymes
Introduction:
In a chemical reaction there sometimes can be a catalyst present known as an enzyme. An enzyme is a protein that speeds up a chemical reaction by lowering the amount of activation energy required to start the reaction. By lowering the activation energy, more of the substrate is able to participate in the reaction, speeding it up. Enzymes are substrate specific. The substrate is what the enzyme bonds to. That is to say that enzyme A will only react with substrate A, enzyme B with substrate B and so on. The enzyme will bond to the substrate’s active site. When the substrate, or reactant and the enzyme bind the ending result is known as …show more content…
The first part of the experiment was broken into three sections. The first section showed the pH and color changes that an enzyme can create. It was predicted that when potato extract, the enzyme, was added to catechol, the substrate, an enzymatic reaction would occur. The second section of the experiment demonstrated enzyme specificity. It was predicted that potato extract, when hydroquinone was introduced, would not exhibit the characteristics of an enzymatic reaction. The third section of part one focused on whether or not concentration matters. It was predicted that the more enzyme the more intense the reaction. The second part of the experiment concentrated on the effects of pH and temperature on an enzymatic reaction. It was predicted that once a solution containing both a substrate and an enzyme reached a certain temperature or pH content, it would not facilitate a chemical …show more content…
Test Tube A contained 5 drops of distilled water, 10 drops of catechol and 5 drops of potato extract. Test Tube B contained 10 drops of distilled water, 5 drops of catechol, and 10 drops of potato extract. Test Tube C contained 5 drops of potato extract and 20 drops catechol. Test Tube D contained 5 drops of distilled water, 20 drops of potato extract and 5 drops of catechol. Test Tube E contained 10 drops potato extract, 4 drops of catechol and 2 drops of water. After 5 minutes two drops from each tube were tested for their pH and results recorded. Any color changes were also