Final Exam: Acids and Enzymatic Reaction
Atausch
July 28, 2014
Bio 102 Lab
Final Exam: Acids and Enzymatic Reaction
Purpose: To examine how an acidic fluid will modify the outcome of an enzymatic reaction.
Introduction: Enzymes are “specialized proteins made by living cells that serve as biological catalysts.” (eScience Labs, LLC, 2013) These enzymes “allow certain industrial processes to be carried out at normal temperatures and pressures, thereby reducing the amount of energy and expensive equipment needed.”(BBC, 2012) Enzymatic activity can be affected by three factors, pH, salt, and temperature. In this project, we will be seeing how acidic fluids will affect the enzymatic reactions. Furthermore, this experiment will answer the following questions: What level of pH there must be in order for the reaction to slow down, or speed up? Are there specific levels of ph levels that speed up enzymes? Seeing that enzymes tend to do great on themselves, my hypothesis is that acid fluids (low pH levels) will actually hinder the enzymatic reaction. My reasoning behind this is that the substrate will take a longer time to mix with the fluid acids, and thus much of the time will be wasted when the enzyme makes contact with the combined substances.
Design:
In order to do this, we will be using the following materials: Yeast Packet (this will be our enzyme, which comes from (source)), 30 mL 3% Hydrogen Peroxide (this will be our substrate), (2) 250 mL Beakers, 3 Balloons, H 2 O 2, Measuring Spoon, Permanent Marker, Ruler, 30 cm String, 3 Test Tubes (Glass), Test Tube Rack, Stopwatch, 3 Acidic Solutions (Orange juice, 4.5% Acetic Acid (Vinegar), C 2 H 4 O, and saliva).
For us to be able to examine how an acidic fluid will modify the outcome of an enzymatic reaction, we will need to measure and see how fast the reaction takes place, and how big the reaction was. Thus, in order for us to do this, we will:
1. Use a permanent marker to label test tubes 1, 2, 3, and 4. Place them in the test tube rack.
July 28, 2014
Bio 102 Lab
Final Exam: Acids and Enzymatic Reaction
Purpose: To examine how an acidic fluid will modify the outcome of an enzymatic reaction.
Introduction: Enzymes are “specialized proteins made by living cells that serve as biological catalysts.” (eScience Labs, LLC, 2013) These enzymes “allow certain industrial processes to be carried out at normal temperatures and pressures, thereby reducing the amount of energy and expensive equipment needed.”(BBC, 2012) Enzymatic activity can be affected by three factors, pH, salt, and temperature. In this project, we will be seeing how acidic fluids will affect the enzymatic reactions. Furthermore, this experiment will answer the following questions: What level of pH there must be in order for the reaction to slow down, or speed up? Are there specific levels of ph levels that speed up enzymes? Seeing that enzymes tend to do great on themselves, my hypothesis is that acid fluids (low pH levels) will actually hinder the enzymatic reaction. My reasoning behind this is that the substrate will take a longer time to mix with the fluid acids, and thus much of the time will be wasted when the enzyme makes contact with the combined substances.
Design:
In order to do this, we will be using the following materials: Yeast Packet (this will be our enzyme, which comes from (source)), 30 mL 3% Hydrogen Peroxide (this will be our substrate), (2) 250 mL Beakers, 3 Balloons, H 2 O 2, Measuring Spoon, Permanent Marker, Ruler, 30 cm String, 3 Test Tubes (Glass), Test Tube Rack, Stopwatch, 3 Acidic Solutions (Orange juice, 4.5% Acetic Acid (Vinegar), C 2 H 4 O, and saliva).
For us to be able to examine how an acidic fluid will modify the outcome of an enzymatic reaction, we will need to measure and see how fast the reaction takes place, and how big the reaction was. Thus, in order for us to do this, we will:
1. Use a permanent marker to label test tubes 1, 2, 3, and 4. Place them in the test tube rack.