Enzyme and Pulpy Pineapple Mixture
Biology, Section G
27 September 2013
Experiment 5.3
Title: The Fragility of an Enzyme
Purpose: To see how easily enzyme function can be destroyed.
Hypothesis: I think that the bowl with only Jell-O will set as will the bowl with heated pineapple but the Jell-O with fresh, uncooked pineapple will not set.
Materials:
Part of a fresh pineapple
A blender or a fine cheese grater
3 small bowls
A small box of Jell-O
Pot
Stove
Refrigerator
2 tablespoons
Procedure:
1. Cut the pineapple to remove any skin. Blend/grate the pineapple into a thick, pulpy mixture of fresh pineapple. You need about a cup of this mixture.
2. Prepare the Jell-O as described in the directions on the box.
3. As you are boiling the water for the Jell-O, take a tablespoon of the thick, pulpy pineapple mixture and pour it into one of the three small bowls. Save that tablespoon for use with that bowl only. Mark the bowl as “room-temperature pineapple juice.”
4. Take the rest of the thick, pulpy mixture and pour it into a pot. You will eventually heat it, but do not do that now.
5. When you have finished preparing the Jell-O up to the point where you stick it in the refrigerator, pour one-third of it into each of the three bowls. Stir the bowl that has Jell-O and the thick, pulpy pineapple mixture with the tablespoon you saved in step (3).
6. Take the pot of thick, pulpy pineapple mixture and heat it on high for five minutes. Keep stirring it constantly, in order to distribute the heat evenly. The thick, pulpy mixture may boil. That’s fine; just keep stirring.
7. After five minutes of heat, take one tablespoon of the hot pulpy mixture and pour it into one of the two bowls that have only Jell-O in them. Use a different tablespoon than the one you used in step (3). Stir vigorously. Label that bowl as “heated pineapple juice.”
8. Put all three bowls in the refrigerator and wait for the amount of time described on the Jell-O box.
9. Examine the three bowls of Jell-O. What
27 September 2013
Experiment 5.3
Title: The Fragility of an Enzyme
Purpose: To see how easily enzyme function can be destroyed.
Hypothesis: I think that the bowl with only Jell-O will set as will the bowl with heated pineapple but the Jell-O with fresh, uncooked pineapple will not set.
Materials:
Part of a fresh pineapple
A blender or a fine cheese grater
3 small bowls
A small box of Jell-O
Pot
Stove
Refrigerator
2 tablespoons
Procedure:
1. Cut the pineapple to remove any skin. Blend/grate the pineapple into a thick, pulpy mixture of fresh pineapple. You need about a cup of this mixture.
2. Prepare the Jell-O as described in the directions on the box.
3. As you are boiling the water for the Jell-O, take a tablespoon of the thick, pulpy pineapple mixture and pour it into one of the three small bowls. Save that tablespoon for use with that bowl only. Mark the bowl as “room-temperature pineapple juice.”
4. Take the rest of the thick, pulpy mixture and pour it into a pot. You will eventually heat it, but do not do that now.
5. When you have finished preparing the Jell-O up to the point where you stick it in the refrigerator, pour one-third of it into each of the three bowls. Stir the bowl that has Jell-O and the thick, pulpy pineapple mixture with the tablespoon you saved in step (3).
6. Take the pot of thick, pulpy pineapple mixture and heat it on high for five minutes. Keep stirring it constantly, in order to distribute the heat evenly. The thick, pulpy mixture may boil. That’s fine; just keep stirring.
7. After five minutes of heat, take one tablespoon of the hot pulpy mixture and pour it into one of the two bowls that have only Jell-O in them. Use a different tablespoon than the one you used in step (3). Stir vigorously. Label that bowl as “heated pineapple juice.”
8. Put all three bowls in the refrigerator and wait for the amount of time described on the Jell-O box.
9. Examine the three bowls of Jell-O. What