Bio Lab

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Santiago NevillePeriod 110/14/12

Enzyme lab
Enzymes are biological catalysts that speed up the process of chemical reactions. They are also proteins, and most enzymes activities occur within organism. They decrease activation energy, energy that is needed to start a chemical reaction. Enzymes are substrate specific substrates ending in "-ase", enzymes ending in "-ase". External factors, such as temperature, pH, and concentration of the substrate, affect the enzymes activity in the lab, pectase and cellulase will be used to compare different enzymes on apple sauce. The lab is designed to investigate the effect of different enzymes on apple juice production The combination of pectinase and cellulase will be the most effective simply because two is better than one. MATERIALS: apple sauce, pectinase enzyme, cellulase enzymes, funnels, filter paper, lab aprons, splash-proof goggles, paper towels, stirring rods, graduated cylinders, access to the water, paperclips, access to a stop watch, and access to a triple bean balance. Procedure:

1) Collect materials
2) Follow safety protocol
3) Measure 1 tablespoon of apple sauce and place it in a cup 4) Add 10 drops of pectinase to the apple sauce and stir for 1 minute 5) Line a funnel with a coffee filter, place funnel in a graduated cylinder, empty the mixture into the funnel, and let it filter for 5 minutes. 6) Record the amount of juice collected

7) Repeat steps 1-5 with cellulase and water.
Enzyme Lab

4

3.2

3.1

3.28

2.4

1.6

Cellulase Pectinase C+P
Water

0.8

0.86

0.52

Amount
of apple juice in ml
Amount
of apple juice in ml

0
Average

Type of Enzyme

Conclusion: Enzymes are catalysts that speed up the process of chemical reactions. They are also proteins, and most Enzymatic activities occur within organism. They decrease the activation energy that is needed to start a chemical reaction. The problem was the effect that different...
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