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Common molecular biology techniques used in health and agricultural sector

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Common molecular biology techniques used in health and agricultural sector
INTRODUCTION:
Molecular biology began more than 40 years ago with Watson and Crick‘s discovery of the double helix structure of deoxyribonucleic acid DNA and that continued with Cohen and Boyer’s. This discovery of the techniques that called as biotechnology nowadays has had an enormous impact on the nature of research and development. This technique has potential to generate great benefits in improved of human life by through of many applications which play role in improvements of medicine, agriculture, food production, environment and others. However, in this article, only health and agriculture sectors which developed from molecular biology techniques were discussed. Recombinant DNA technology is the one that is widely used in biotechnology fields which is resulting recombinant of proteins and other products that result from the use of rDNA that is found in essentially on every health and agriculture sector. The most common application of recombinant DNA technologies that was used in health sector for example are human insulin and hepatitis B vaccine. Recombinant DNA human insulin developed by molecular biology technique gives huge contribution to health sector in order to produce biosynthetic insulin protein which helps control level of sugar in diabetes patients, who are disable or do not correctly produce their insulin from their body. Insulin is the internal secretion of the pancreas formed by groups of cells called the islets of Langerhans. It is the hormone needed to enable glucose to enter the cells and provide energy and also important in keeping blood glucose levels within the acceptable limits. The biosynthetic insulin which produced through recombinant DNA technology is injected into the body by people who suffered from diabetes with type 1 diabetes in whom the cells that produce insulin have been destroyed. This is the most common form of diabetes in children and young adults, and they depend on insulin for survival. Insulin may also be used by



References: Abraham et al. (1999). Evaluation of a new recombinant DNA hepatitis B vaccine (Shanvac-B). Science Direct. 9 (17): 125–1129. Jouanin L. (1998) et al. (1998). Review Transgenic plants for insect resistance, Plant Science, Versailles 131:1-11. Keefer et al. (2001). Recombinant DNA Technology in the Treatment of Diabetes: Insulin Analogs. Endocrine society. 22(5):706–717. Lemaux, Peggy G. (2008). "Genetically Engineered Plants and Foods: A Scientist 's Analysis of the Issues (Part I)". Annual Review of Plant Biology. 59:771-812.doi:10.1146/annurev.arplant.58.032806.103840. PMID 18284373. Miller H.I., (2009). A golden opportunity, squandered. Hoover Institution, Stanford University, USA. Trends in Biotechnology 27(3). Vaeck, Mark; Reynaerts, Arlette; Höfte, Herman et al. (1987). "Transgenic plants protected from insect attack". Nature 328 (6125): 33–7. Bibcode:1987Natur.328...33V. doi:10.1038/328033a0. World Health Organization, (1985). Hepatitis B vaccines prepared from yeast by recombinant DNA techniques. National institute of Health. 63(1): 57 – 61.

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