Preview

Chemical Aspects of Life and Spit Lab Formal Report

Powerful Essays
Open Document
Open Document
2424 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Chemical Aspects of Life and Spit Lab Formal Report
Chemical Aspects of Life and Spit Lab Formal Report
1. Abstract
The objective of the Reducing Sugar Test was to test if the substance has a reducing sugar in it by adding Benedict’s solution and heating it, there would be a color change if a reducing sugar is present, or it will remain blue (no reducing sugar). The objective of the Starch test was to test for starch in substances by using Iodine. The iodine will cause a substance to turn to a dark blue color if it is positive for starch. The objective for the Grease Spot Test was to test if the substance had a lipid. A positive reaction would make a translucent grease mark on a brown paper bag. The objective for the Dye test was to test for lipids. When mixed with water and the tested substance, a positive result will occur in it being separated from the water. The objective of the Protein Test was to test for proteins present in a substance using the Biuret Solution. The substance should produce a violet color within 10 drops of Biuret. The objectives of the Spit Lab were to test for starch, a reducing sugar, and effect of amylase on a cracker that is positive for starch and negative for a reducing sugar. Iodine would test if starch was present if the cracker solution changes to a dark black/blue color. Benedict’s Solution mixed with the cracker and heated would test for a reducing sugar (if reducing sugar, it will turn from a green to an orange to a dark brownish color). The amylase was tested on a Triscuit cracker by heating it in Benedict’s Solution, the amount would make it either a green, or orange, and the most amounts would make it brown as mine and my partner’s results.
1. Introduction
The chemical aspects lab was done to detect and identify the presence of reducing sugars, starches, lipids, and proteins in various substances. A reducing sugar is a monosaccharide or disaccharide that has the ability of giving electrons to other molecules and acts as reducing agent. A reducing agent has an aldehyde or

You May Also Find These Documents Helpful

  • Satisfactory Essays

    Exp 1

    • 654 Words
    • 5 Pages

    The objective of this experiment was to observe how different chemicals react. I was able to see how acids react with other compounds. When testing the foods with NaOCl and KI solutions, I was able to determine which foods around the house had starch. The chip, tortilla and hot dog bun immediately turned black once the KI solution was dropped. The potato turned a lighter black, and it took awhile for the color to show up. This could be a result of the potato being so dense. The lettuce turned a darker green…

    • 654 Words
    • 5 Pages
    Satisfactory Essays
  • Satisfactory Essays

    Lab 2

    • 559 Words
    • 3 Pages

    In the test for starches, water again will serve a the negative control for the experiment becasue it is inorganic. it appears obvious the 1% starch solution will test positve for starches becasue it is starch. The onion juice should carry starch due to the fact that starch is stored energy for plants and onions are very bulbous, while the sucrose solution again will not test positive because sucrose is only a part of starch.…

    • 559 Words
    • 3 Pages
    Satisfactory Essays
  • Satisfactory Essays

    * When the solution was added to the hot water bath, the solution went from blue, to green, to yellow, and finally to orange as time progressed. This was a positive result, meaning that the amylase in our saliva broke the polysaccharides in the cracker into mono- and di- saccharides. As a result, the Benedict’s reagent changed colour because it indicated the presence of mono- and di- saccharides in the solution.…

    • 433 Words
    • 2 Pages
    Satisfactory Essays
  • Satisfactory Essays

    Physioex Exercise 8

    • 521 Words
    • 3 Pages

    Experiment Results Predict Question: Predict Question: Do you think test tube 3 will show a positive Benedict's test? Your answer : a. yes Stop & Think Questions: You included tube 2 to Your answer : d. make sure there is no contaminating glucose in the amylase. Correct answer: b. see what a positive Benedict's test should look like. Which tube is included to detect contaminating amylase in the buffer or in cellulose? Your answer : b. tube 2 Correct answer: d. tube 4 What is the usual substrate for the pancreatic enzyme peptidase? Your answer : a. starch Correct answer: d. peptides Experiment Data: Tube No. 1 2 3 4 5 6 Reagent 1 Amylase Amylase Amylase Cellulose Peptidase Bacteria Reagent 2 Starch Glucose Cellulose Water Starch Cellulose Reagent 3 pH 7.0 pH 7.0 pH 7.0 pH 7.0 pH 7.0 pH 7.0 Time 60 60 60 60 60 60 Temp. 37 37 37 37 37 37 IKI + + + Benedict's ++ ++ ++…

    • 521 Words
    • 3 Pages
    Satisfactory Essays
  • Good Essays

    Enzyme Lab Write Up

    • 724 Words
    • 3 Pages

    We then repeated this in two more test tubes. We put one of the test tubes in ice (0 C) we put another in the water bath (42 C) then we put one in boiling water (100 C). We repeated the same thing for the boiled amylase. We then checked at five minutes to see if the starch had been digested by the amylase. We did this by putting a little of each of the six samples into a spotting tile. We then added iodine solution to this to see if starch was present or not. I could tell if starch was present or not because if the solution went orange it meant that there was no starch present, so all the starch had been digested, if the solution went blue/black it meant that there was starch present, so the starch had not been fully digested. We recorded the results in a table. We then waited another two minutes so that all together the starch solution had been in there seven minutes. We did exactly the same thing as we did at five minutes we also recorded these results in a…

    • 724 Words
    • 3 Pages
    Good Essays
  • Good Essays

    A few experiments were performed to test for the presence of the different types of macromolecules of various solutions. The iodine tested for starch and glycogen, Benedict’s test tested for reducing sugars for carbohydrates and biuret test was performed to test for proteins. It is necessary to understand how important controls are in a lab experiment. A control is where all of the experimental variables can be controlled and monitored. Results are determined by comparing these variables to the control. Without them, no results would be valid in the experiment. To tell whether or not the experimental procedure were carried out properly and correctly, treatment controls were used. They can either be positive or negative. Correct data with followed procedures, resulting in a working experiment would be a positive control. On the opposite hand, negative controls would give negative results if everything were to be followed in the experiment.…

    • 1158 Words
    • 5 Pages
    Good Essays
  • Good Essays

    The common organic compounds provide us with nutrients are sugars and starches, carbohydrates, proteins, and lipids. In this lab, qualitative tests are performed to identify the presence of organic compounds in food using indicators, chemical substances that react in a certain way when a particular substance is present. We put one indicator into the each test tube containing different solutions at a time to see what organic compound is present. The color change in the indicator tells that cornstarch contains starch; honey, lettuce, and fruit juice contain sugar; corn oil contain lipid; and by comparing with the standard for positive identification of the substance, unknown is tested to have protein just as egg white. The result tells what nutrients are in the food, and how to test the unknown substances.…

    • 797 Words
    • 3 Pages
    Good Essays
  • Powerful Essays

    Lab Report for Food Test

    • 1475 Words
    • 6 Pages

    Carbohydrates are the product that made up from carbon, hydrogen and oxygen. Carbohydrates are form by the combination of carbon dioxide and water molecules. The carbohydrates contain two specific functional group in it which is the hydroxyl groups and carbonyl groups.A reducing sugar is a type of sugar with is an aldehyde group.This means that sugar can act as a reducing agent.The procces of reducing sugar is isomerisation,example of reducing sugar islactose,maltose,glucose and fructose.All monosaccharides are capable of reducing other chemicals such as copper (II) sulphate to copper oxide.Beside that disaccharides such as maltose and lactose are reducing sugar,however sucrose is non reducing sugar.Sugar exist in solution as an equliburum mixture of open chain and closed ring or in other words is cyclic structures.In the open chain form,the carbon contains special bond called carbonyl carbon.In closed ring form or cyclic structure the carbonyl carbon is the one attached to the (O) of the ring and OH group.This sugars is called reducing sugar because the oxidising agent is reduce during the reaction.Therefore non reducing sugar cannot be oxidised my mild oxidising agent. When the closed-ring (cyclic) structure of a monosaccharide opens to form a chain, the result can be divided into two which is an aldehyde or an ketone. Sugars that are aldehydes are known as aldoses. Sugars that are ketones are known as ketoses.Glucose and galactose are example of aldoses while fructose is an example of ketoses.There are few test can be done for testing these sugar to show or prove are they reducing sugar or non reducing sugar.Example of the test that can be done is Benedict test. 1…

    • 1475 Words
    • 6 Pages
    Powerful Essays
  • Good Essays

    A reducing sugar is any sugar that, in basic solution, forms some aldehyde or ketone. This allows the sugar to act as a reducing agent, for example in the Maillard reaction and Benedict's reaction. Reducing sugars include glucose, glyceraldehyde, lactose, arabinose and maltose. All monosaccharides which contain ketone groups are known as ketoses, and those which contain aldehyde groups are known as aldoses. Significantly, sucrose is not a reducing sugar. It is in fact known as a non-reducing sugar.…

    • 324 Words
    • 2 Pages
    Good Essays
  • Good Essays

    Objective : To investigate the action of saliva and 3M hydrochloric acid in two carbohydrate…

    • 926 Words
    • 4 Pages
    Good Essays
  • Good Essays

    Biochemical Tests

    • 949 Words
    • 4 Pages

    Reducing sugar have contain free aldehyde or ketone group and have the ability to reduce copper(II) ions to copper (I) ions during the Benedict's Test. All monosaccharides are reducing sugars while only some dissacharides are reducing sugar.…

    • 949 Words
    • 4 Pages
    Good Essays
  • Good Essays

    Should Girl Ask Boys Out

    • 326 Words
    • 2 Pages

    Introduction: In this lab we will be detecting biological molecules using testing methods. Here we will be testing some food products for sugar, starch, protein, and lipids. We will be testing each food product with Benedict’s, Lugol’s, Ninhydrin, and Albumin solutions. If when we test each substance and the color changes, then that product has one or some of sugar, starch, protein, or lipids in it.…

    • 326 Words
    • 2 Pages
    Good Essays
  • Good Essays

    Miss

    • 1825 Words
    • 8 Pages

    Aims: 1) To learn how to carry out biochemical tests to identify biochemicals in pure form.…

    • 1825 Words
    • 8 Pages
    Good Essays
  • Good Essays

    Acetic Acid in Vinegar

    • 897 Words
    • 4 Pages

    To test for the presence of glucose, Benedict’s solution was used to test for reducing sugars. Benedict’s reagent is clear blue due to the copper…

    • 897 Words
    • 4 Pages
    Good Essays
  • Good Essays

    Being familiar with the background information about reducing sugars and various methods used to identify them,Biotechnology students were provided with a Fructose sample solution and were required to find its concentration…

    • 602 Words
    • 3 Pages
    Good Essays