07/17/12
page 1
Experiment Results Predict Question: Predict Question: Do you think test tube 3 will show a positive Benedict's test? Your answer : a. yes Stop & Think Questions: You included tube 2 to Your answer : d. make sure there is no contaminating glucose in the amylase. Correct answer: b. see what a positive Benedict's test should look like. Which tube is included to detect contaminating amylase in the buffer or in cellulose? Your answer : b. tube 2 Correct answer: d. tube 4 What is the usual substrate for the pancreatic enzyme peptidase? Your answer : a. starch Correct answer: d. peptides Experiment Data: Tube No. 1 2 3 4 5 6 Reagent 1 Amylase Amylase Amylase Cellulose Peptidase Bacteria Reagent 2 Starch Glucose Cellulose Water Starch Cellulose Reagent 3 pH 7.0 pH 7.0 pH 7.0 pH 7.0 pH 7.0 pH 7.0 Time 60 60 60 60 60 60 Temp. 37 37 37 37 37 37 IKI + + + Benedict's ++ ++ ++
07/17/12
page 2
Post-lab Quiz Results You scored 25% by answering 1 out of 4 questions correctly. 1. The substrate(s) for amylase is/are Your answer: b. cellulose. Correct answer: a. starch. 2. The results of tube 5 demonstrated that You correctly answered: b. peptidase does not digest starch. 3. Why was the cellulose in tube 6 hydrolyzed to glucose? Your answer: b. The bacterial suspension contained reducing sugars. Correct answer: d. The bacterial suspension contained the enzyme cellulase, which digested the cellulose. 4. Can you detect the presence of contaminating amylase from your experiments? Your answer: d. There was no contaminating amylase because tube 4 was positive. Correct answer: c. It is not possible to determine whether contaminating amylase is present because amylase doesn't digest cellulose.
07/17/12
page 3
Review Sheet Results 1. Describe why the results in tube 1 and tube 2 are the same. You did not answer this question. 2. Describe the result in tube 3. How well did the results compare with your prediction? Your answer: I was correct. 3. Describe the usual substrate for peptidase. You did not answer this question. 4. Explain how bacteria can aid in digestion. You did not answer this question.
07/17/12
page 4
You May Also Find These Documents Helpful
-
E) If he only enzymes came from his mouth he would be able to digest starch because even if food is swallowed fast chewing it breaks it down with salivary amylase and continues to act on the starch as it continues…
- 505 Words
- 3 Pages
Satisfactory Essays -
Which way would Na+ move across the membrane if there wer open Na channels? A. into the cell…
- 2112 Words
- 9 Pages
Powerful Essays -
12. What is the substrate in this experiment? What is the usual substrate for pepsin, and what subunits are formed with pepsin activity?…
- 259 Words
- 2 Pages
Satisfactory Essays -
We then repeated this in two more test tubes. We put one of the test tubes in ice (0 C) we put another in the water bath (42 C) then we put one in boiling water (100 C). We repeated the same thing for the boiled amylase. We then checked at five minutes to see if the starch had been digested by the amylase. We did this by putting a little of each of the six samples into a spotting tile. We then added iodine solution to this to see if starch was present or not. I could tell if starch was present or not because if the solution went orange it meant that there was no starch present, so all the starch had been digested, if the solution went blue/black it meant that there was starch present, so the starch had not been fully digested. We recorded the results in a table. We then waited another two minutes so that all together the starch solution had been in there seven minutes. We did exactly the same thing as we did at five minutes we also recorded these results in a…
- 724 Words
- 3 Pages
Good Essays -
Enzymes are biological catalysts. They speed up chemical reactions in all living things, and allow them to occur more effortlessly, without them we would not be alive. “Catalyst” denotes a substance that has the ability to increase the rate of a chemical reaction, and is not changed or destroyed by the chemical reaction that it accelerates (MicroTak, 2002). The enzyme Amylase speeds up the breakdown of starch into simple sugar; this reaction happens in the mouth and is the start of chemical digestion. Starch cannot pass through the lining of the intestine; it is too big (REFER TO FIGURE-31). The enzyme in question here is called Amylase; it catalyzes the hydrolysis of the polysaccharide starch (amylose) to the disaccharide maltose (Miller, 1992 ).…
- 6364 Words
- 26 Pages
Good Essays -
The purpose of this experiment is to identify three unknown enzymes. This is done by…
- 955 Words
- 4 Pages
Better Essays -
7.0 because that is when the salivary is most effective and it breaks down carbohydrates.…
- 705 Words
- 3 Pages
Good Essays -
The purpose of this lab experiment is to investigate factors that can affect the porcine pancreatic amylase enzyme activity in different environments such as the temperature, pH and also how being stored in extreme temperatures can affect the activity of the amylase. The activity of the amylase is going to be determined by the presence or absence of starch in the samples over time. There are some hypotheses on the Effects of temperature and pH; as I add the amylase to the starch in different temperatures the reaction’s rate increases in high temperatures; I belive that the amylase will work better. As the environment grows warmer, the amylase is going to become more energetic and more effective. Amylase is affected by environmental pH. I predict that the amylase activity will work best at a pH 7. As the pH changes from this point I predicted that the amylase activity is going to decrease and eventually stop. If I boiled and froze some amylase solution, and try to digest starch with at it at room temperature, I predict the previously-boiled and frozen amylase will not…
- 1766 Words
- 8 Pages
Better Essays -
Exercise 8: Chemical and Physical Processes of Digestion: Activity 1: Assessing Starch Digestion by Salivary Amylase Lab Report Pre-lab Quiz Results You scored 100% by answering 6 out of 6 questions correctly. 1. The substrate for amylase is You correctly answered: e. starch and carbohydrate. 2. Which of the following is true of enzymes? You correctly answered: c. Their activity can be affected by temperature and pH. 3. The reagent IKI tests for the presence of You correctly answered: a. starch. 4. Which of the following is not true of controls? You correctly answered: d. A negative result with a positive control is required to validate the test 5. Which of the following is an end product of starch digestion? You correctly answered: e. maltose and glucose 6. Hydrolases are enzymes that break down large molecules into smaller subunits through the addition of _____________. You correctly answered: b. water…
- 622 Words
- 3 Pages
Satisfactory Essays -
The purpose of this lab was to check the time needed for salivary amylase and phosphorylase to produce a negative result and how different concentrations affected those times. Enzymes are biological catalysts that can cause a specific chemical change in any part of the body (Walsh, 2002). Many of the reactions that take place within a cell would normally take place at temperatures substantially higher than those present inside a cell (Alberts et al., 2010). Because of this, these chemical reactions require the assistance of enzymes to catalyze the reaction and allow it reaction to occur quickly (Alberts et al., 2010). The substrate binds with the active site on the enzyme to form a substrate enzyme complex (Di Giuseppe, 2002). The substrate is then transformed into one or more products, which are released from the active site and the enzyme remains unaltered (Di Giuseppe, 2002).…
- 2321 Words
- 9 Pages
Better Essays -
What do tubes 2, 6, and 7 reveal about pH and amylase activity? Hint: What variable was changed in the procedure?…
- 858 Words
- 4 Pages
Good Essays -
a. How do (1) temperature and (2) pH affect the activity of this enzyme? In your answer,…
- 2259 Words
- 10 Pages
Powerful Essays -
In these labs, my partner and I had to understand, and make note of, the types of reactions that occurred in each test tube. These reactions would be brought on by enzymes that would interact with other substances. [Part A] In this lab, we hypothesized that the tubes that contained a pancreatic solution would dissolve the starch, while the other test tubes wouldn’t. This is because the pancreatic solution contains enzymes which complete the digestion of carbohydrates, proteins, and lipids (Digestion BioKit). Although test tube three contained this solution, we predicted that there wouldn’t be a reaction due to the fact that it was being boiled. The number, type, and arrangement of the amino acids determine the shape of the protein which directly controls the protein's function. The shape and function of a protein can be altered by a process known as denaturing. Too high a temperature or change of pH can cause the denaturing to take place. If this happens, then the protein can be rendered useless, unable to perform its normal job. In this…
- 1604 Words
- 7 Pages
Better Essays -
To test the effect of a substrate concentration on enzyme activity, the amylase enzymes were combined with a different substrate concentration (starch) and the rate of the reaction was determined with the aid of I2kI. If starch was detected, the solution turned to dark blue; if the starch was already broken down, then reaction stayed colorless. To test the optimal PH, the starch and a buffer were combined at a specific PH level and the rate of reaction was tested. To determine the optimal temperature of amylase enzyme, the solution and amylases enzyme were held at various temperatures and the rate of reaction was determined.…
- 2678 Words
- 11 Pages
Better Essays -
2. List whether each of the following substances was positive or negative for reducing sugar, as indicated by the Benedict's test. (6 points)…
- 941 Words
- 4 Pages
Satisfactory Essays