Catalase Rate Lab Report

Variable Measured How variable was controlled
Independent Variable: Temperature (⁰C) of the catalase and H2O2 Both of the solutions will be poured into test tubes/beakers and then cooled and heated to certain temperatures (10, 22, 30, 37, 45, and 60⁰C) and with the use of ice and a water bath ( in a separate beaker) I attempted to control the temperature of the catalase mixture as well as the H2O2 solution
Dependent Variable: Rate of reaction (cm3/second) The gas syringe (± 0.5) is used to measure the amount of oxygen produced from the catalase reaction. A rubber stopper is used to ensure that there is a closed system and no O2 escapes
Controlled Variables: Substrate ( H2O2 ) Concentration and Quantity The same pipette will be used to measure
A timer will be used to ensure that the recording does not go beyond 10.0 seconds. The rate of the reaction will be recorded every 5 seconds using a timer(±
The same syringe and needle will be used for all trials to ensure consistency of measurements pH = 7 The catalase solution used for the trials is from the same original catalase blended mixture. I attempted to keep the pH of the catalase mixture at 7 by using a buffer solution. I did this in order to control the pH level and ensure that it did not vary during the experiment. Type of Liver (source of catalase) One lamb liver will be used for all the catalase solutions.
Moreover, the liver will be kept in the fridge when not in use so that the enzyme does not denature due to the temperature shifts. Amount of time that the catalase mixture was blended The catalase mixture was blended for approximately 4 minutes and 50 seconds. This was ensured using a timer (± 0.005).
This time seemed the most appropriate because the mixture looked smooth and seemed to not have that many chunky pieces
Table 1- Independent, Dependent and Controlled Variables
METHOD
Apparatus/ Materials:
(1) Lamb Liver (approximately 500 grams)
(1) 100.0 ml Gas syringe (±