Catalase Lab Report

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The Catalase Lab
Stephen
Human Anatomy & Physiology 9/30/12

Problem- How do different environments affect the reactivity of catalase?

Hypothesis- If more catalase is added then more oxygen (kPa) will be produced in a faster rate because there is more catalase to react upon. If less catalase is added then less oxygen (kPa) will be produced in a slower rate because there is less catalase to react upon.

Variable-
Independent- Amount of Catalase (Filter Paper)
Dependent- Amount of Oxygen (kPa)
Constant- Temperature in Fahrenheit, 2 Pipette of Hydrogen Peroxide, 0.8 Cm Filter Paper Punches
Materials:
* 6 Test Tubes
* Vernier Gas Pressure
* Sensor
* Catalase
* Filter Paper Punches
* Beaker
* Control Group
* Test Subject
* Safety Goggles
* Dropper Pipette
* Go Link

Procedure-

1. Gather Your Materials Figure 2
2. Put on your goggles
3. Login into your computer Figure 1 4. Open up Logger Pro
5. Follow directions on the Vernier Direction sheet
6. Plug in the Go Link onto the USB drive of the computer (See Figure 1) 7. Plug in the Vernier Gas Pressure Sensor onto the Go Link (See Figure 2) 8. Grab 2 Test Tubes and follow Steps 1-6 on the regular procedure on Enzymes in Action sheet (See Figure 3 & 4) 9. Record your Data after 1 Minute and 30 Seconds

10. Grab 2 Test Tubes and follow Steps 1-6 on the regular procedure on Enzymes in Action sheet EXCEPT using 5 Filter Paper Punches (See Figure 3 & 4) 11. Record your Data after 1 Minute and 30 Seconds

12. Grab 2 Test Tubes and follow Steps 1-6 on the regular procedure on Enzymes in Action sheet EXCEPT using 1 Filter Paper Punch (See Figure 3 & 4) 13. Record your Data after 1 Minute and 30 Seconds

14. Clean up your work station
15. Before pouring the Hydrogen Peroxide down the sink, get the filter paper out first 16. After washing the test tubes, put...
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