Biological Assessment of Paris Polyphylla
Biological Activities of Paris polyphylla found in Himalayan Region of Nepal
Prabin Koirala, Khagesh Chataut and Keshab Bhattarai
Department of Biotechnology, WhiteHouse Institute of Science and Technology, Purbanchal University, Lalitpur, Nepal. e-mail: prabinkoirala122@gmail.com
Abstract
The study focused on phytochemical and biological analysis of Paris polyphylla (PP). Crude Chloroform/Methanol (1:1) extraction performed on the rhizomes of Paris polyphylla collected from himalayan region of Nepal was followed by fractionaion process using petroleum ether and acetone for the separation of extract based on their polarity. The extract was run through Thin Layer Chromatography and the test confirmed the presence of polar and Non-polar compounds based on their mobility. Phytochemical screening was performed and presence of different secondary metabolites and compounds like saponin, reducing sugars, Steroids and terpenoids were confirmed. Antimicrobial activity was performed on various fractionated extracts against five pathogenic microorganisms (Bacillus subtilis, Staphylococcus aureus, klebsiella species, Escherichia coli and Salmonela paratyphi). The zone of inhibitions shown by Acetone soluble (PPAs) and acetone insoluble (PPAins) extract when compared to that of standard antibiotic (streptomycin), in which gram positive bacteria (Bacillus subtilis and Staphylococcus aureus) were found effective in ubiquitos of samples (10%PPAs and 15%PPAins), however klebsiella spp. were effective on 15%PPAins only. Brine Shrimp Lethality test was performed on nauplii from which the LC50 values were calculated as 158.48ppm and 125.89ppm for PPAs and PPPs extracts of Paris polyphylla respectively. Thus, effect of petroleum soluble extract was found to be more lethal than other extracts. Key words: Fractionation, Phytochemical Screening, Antimicrobial activity, TLC, Brine Shrimp Lethality Assay.
Introduction
Natural product is a chemical substance found in nature
Prabin Koirala, Khagesh Chataut and Keshab Bhattarai
Department of Biotechnology, WhiteHouse Institute of Science and Technology, Purbanchal University, Lalitpur, Nepal. e-mail: prabinkoirala122@gmail.com
Abstract
The study focused on phytochemical and biological analysis of Paris polyphylla (PP). Crude Chloroform/Methanol (1:1) extraction performed on the rhizomes of Paris polyphylla collected from himalayan region of Nepal was followed by fractionaion process using petroleum ether and acetone for the separation of extract based on their polarity. The extract was run through Thin Layer Chromatography and the test confirmed the presence of polar and Non-polar compounds based on their mobility. Phytochemical screening was performed and presence of different secondary metabolites and compounds like saponin, reducing sugars, Steroids and terpenoids were confirmed. Antimicrobial activity was performed on various fractionated extracts against five pathogenic microorganisms (Bacillus subtilis, Staphylococcus aureus, klebsiella species, Escherichia coli and Salmonela paratyphi). The zone of inhibitions shown by Acetone soluble (PPAs) and acetone insoluble (PPAins) extract when compared to that of standard antibiotic (streptomycin), in which gram positive bacteria (Bacillus subtilis and Staphylococcus aureus) were found effective in ubiquitos of samples (10%PPAs and 15%PPAins), however klebsiella spp. were effective on 15%PPAins only. Brine Shrimp Lethality test was performed on nauplii from which the LC50 values were calculated as 158.48ppm and 125.89ppm for PPAs and PPPs extracts of Paris polyphylla respectively. Thus, effect of petroleum soluble extract was found to be more lethal than other extracts. Key words: Fractionation, Phytochemical Screening, Antimicrobial activity, TLC, Brine Shrimp Lethality Assay.
Introduction
Natural product is a chemical substance found in nature
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