P Guajava Lab Report
2.2.3 P. guajava :-
P. guajava leaf extracts were subjected to inhibitory effect of glucose utilization using specific standard in vitro procedure. Plant material was subjected to the extraction preparation by soxhlet apparatus by using various solvents such as aqueous, ethanol, chloroform, petroleum ether and hexane. The various kinds of phytochemicals were detected and then in vitro antidiabetic activity of P. guajava were detected by using alpha amylase and alpha glucosidase enzyme in an in vitro model.The study reveals the presence of phytochemicals such as carbohydrate, tannin, flavonoids, phenols etc., Among the various extracts the aqueous and ethanolic extracts which contains the large number of phytoconstituents. The P. guajavaleaves …show more content…
Similar diet is given to all the group describe bellow including this group of mice as per guideline of CPCSEA. This group has not given any of drug or exposure after inducing diabetes. All the mice are positively increase the blood sugar level. During the experiment one mouse was died on 7th day. One other group having three mice was administrated with known drug (Glimepiride) given the positive response towards the reduction of blood glucose level in all the mice. In this experiment one mice was died followed by 7th day. Average blood glucose of all the mice was taken for plotting the graph. A.N. Nagappa at al,2003, study the effect of the petroleum ether, methanol, and aqueous extracts of Terminalia catappa Linn (combretaceae) fruit, on fasting blood sugar levels and serum biochemical analysis in alloxan-induced diabetic rats were investigated. All the three extracts of Terminalia catappa produced a significant antidiabetic activity at dose levels 1/5 of their lethal doses. Concurrent histological studies of the pancreas of these animals showed comparable regeneration by methanolic and aqueous extracts which were earlier, necrosed by alloxan. Rao and Naidu (2010) suggested that the plant extract (methanolic) of Rhinacanthusnasutus was capable of ameliorating at the dosage of 200 mg/kg, hyperglycaemia in streptozotocin-induced diabetic rats and could be a potential source for isolation of new orally active agent(s) for anti-diabetic therapy. Mohammed Fazil Ahmed et al,2010, conclude that alcoholic whole plant extracts of Vincarosea at high dose (500mg/kg) exhibited significant antihyperglycemic activity. These extracts also showed improvement in parameters like body weight and lipid profile as well as regeneration of cells of pancreas and so might be of value in diabetes treatment. Further investigation is in necessary to determine the exact phytoconstituents (s) responsible for antidiabetic
P. guajava leaf extracts were subjected to inhibitory effect of glucose utilization using specific standard in vitro procedure. Plant material was subjected to the extraction preparation by soxhlet apparatus by using various solvents such as aqueous, ethanol, chloroform, petroleum ether and hexane. The various kinds of phytochemicals were detected and then in vitro antidiabetic activity of P. guajava were detected by using alpha amylase and alpha glucosidase enzyme in an in vitro model.The study reveals the presence of phytochemicals such as carbohydrate, tannin, flavonoids, phenols etc., Among the various extracts the aqueous and ethanolic extracts which contains the large number of phytoconstituents. The P. guajavaleaves …show more content…
Similar diet is given to all the group describe bellow including this group of mice as per guideline of CPCSEA. This group has not given any of drug or exposure after inducing diabetes. All the mice are positively increase the blood sugar level. During the experiment one mouse was died on 7th day. One other group having three mice was administrated with known drug (Glimepiride) given the positive response towards the reduction of blood glucose level in all the mice. In this experiment one mice was died followed by 7th day. Average blood glucose of all the mice was taken for plotting the graph. A.N. Nagappa at al,2003, study the effect of the petroleum ether, methanol, and aqueous extracts of Terminalia catappa Linn (combretaceae) fruit, on fasting blood sugar levels and serum biochemical analysis in alloxan-induced diabetic rats were investigated. All the three extracts of Terminalia catappa produced a significant antidiabetic activity at dose levels 1/5 of their lethal doses. Concurrent histological studies of the pancreas of these animals showed comparable regeneration by methanolic and aqueous extracts which were earlier, necrosed by alloxan. Rao and Naidu (2010) suggested that the plant extract (methanolic) of Rhinacanthusnasutus was capable of ameliorating at the dosage of 200 mg/kg, hyperglycaemia in streptozotocin-induced diabetic rats and could be a potential source for isolation of new orally active agent(s) for anti-diabetic therapy. Mohammed Fazil Ahmed et al,2010, conclude that alcoholic whole plant extracts of Vincarosea at high dose (500mg/kg) exhibited significant antihyperglycemic activity. These extracts also showed improvement in parameters like body weight and lipid profile as well as regeneration of cells of pancreas and so might be of value in diabetes treatment. Further investigation is in necessary to determine the exact phytoconstituents (s) responsible for antidiabetic