bacteria were obtained from various sources. Two bacterial colonies were isolated from plates that were incubated in the lab and these bacteria became the basis for this project. The bacterial cultures were maintained and used for various physiological‚ cultural‚ and biochemical tests and observations over several weeks. The purpose of culturing bacteria over half the course of the semester was to learn various techniques used in microbiology labs and to subsequently utilize those techniques for
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outcomes of spontaneous bacterial peritonitis in Egypt‚ single center experience prospective study Spontaneous bacterial peritonitis (SBP) is the infection of the ascetic fluid that occur in the absence of a visceral perforation and in the absence of an intraabdominal inflammatory focus such as abscess‚ acute pancreatitis or cholecystitis (Guarner C and Soriano G‚ 2007). Since its initial description in 1964‚ research has transformed spontaneous bacterial peritonitis (SBP) from a
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The following show expected colony appearances and morphologies (shapes) of Escherichia coli and Staphylococcus aureus. Note characteristics such as edges‚ color‚ and whether the colonies are rough or smooth in texture. For colony appearances of E. coli and S. aureus‚ scientists often describe what they look like on agar. This is not the microscopic view (for example‚ as with a slide) but a “naked eye” view of how the bacterial colonies look while growing on a medium. (This is one type of culture
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(Iodine) are an appropriate antiseptic to can kill a sample bacterial contaminant found in the local environment‚ and if so‚ which is the most effective? The purpose of this experiment is to determine the antibacterial effectiveness of Scope‚ Listerine‚ Bactine‚ and Iodine in relation to each other. Scope‚ Listerine‚ Bactine‚ and Iodine each are believed to be effective antiseptics: * Scope Mouthwash - Kills 99% of bad breath germs (in lab tests) http://www.scopemouthwash.com/scope-products/scope-mouthwash-original-mint
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Bacterial growth patterns and the effects of environmental factors on properties of colonies ERIC LI Abstract In this experiment we work with different types of bacteria and experiment with their different properties and how they grow in certain situations. Different samples of bacteria cultures were gathered from different places such as the mouth‚ shaded mulch‚ and from the table top. The samples were collected by using a cotton swab and swiped onto a petri dish filled with nutrient agar. The
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Bacterial Genetics Worksheet 1. Fill in: Transformation Transduction Conjugation a. Transformation _ naked DNA is involved b. Transduction ____faulty head stuffing may occur c. Conjugation_____ involves exconjugants d. Transduction____ adsorption and injection of genetic material e. Transformation__ competent cells required f. Conjugation ____ an F plasmid encodes attachment proteins g. Conjugation _____used to construct the E. coli minute map h. Conjugation_____
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Practical 9: Examination of Bacterial Plates Objective: 1. To examine bacteria pigmentation‚ colony‚ margin characteristics‚ elevation properties‚ broth characteristics and agar stroke properties. 2. To examine bacteria growth characteristics on different culture media. Introduction: Bacterial species can sometimes be identified on the basis of how they appear on or in the different media. The pigmentation‚ size and shape of bacterial colonies as they grow on and in agar plates can
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Bacterial Energetics and Membranes Abstract The Mg2+/Ca2+ ATP synthase present in all bacterial membranes‚ particularly E. coli‚ couples ATP synthesis to the proton (H+) gradient produced by the ETC‚ a process known as oxidative phosphorylation. The gradient acts to power the ATPase‚ so that it may phosphorylate ADP to produce ATP. The reverse reaction of this process‚ or hydrolysis of ATP into ADP and Pi‚ may be used to observe ATPase activity when the resulting Pi is quantitatively measured
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Ensuring the Social‚ Political‚ and Technological Protection of Water Quality Protecting Against Bacterial‚ Parasitic‚ and Protozoan Pathogens Nathan Pankowsky Contents Abstract....................................................................................................................................................... 2 Introduction ............................................................................................................................................... 3
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Bacterial Growth Requirements Evelyn Lyle ITT Technical Institute Angela Ask‚ MPS January 15‚ 2012 Every organism must find in its environment all of the substances required for energy generation and cellular biosynthesis. The chemicals and elements of this environment that are utilized for bacterial growth are referred to as nutrients. Many bacteria can be identified in the environment by inspection or using genetic techniques. The nutritional requirements of a bacterium such as E Coli are
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