Bacterial Morphology MLT1 Task 11- Lab 2 Johnny Archuleta Western Governors University Question A and C answers. A wet mount stain is when a drop of water is placed onto the microscope slide. The water on the slide helps to support the organism and sample. The water fills the space between the cover slip and the slide. This action allows the light from the microscope to pass through the slide and the sample for better visualization of the organisms. A direct stain occurs when a charged
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Lab‚ Week ASEPTIC TECHNIQUE AND BACTERIAL ANATOMY AND MORPHOLOGY Introduction Part I: Aseptic Technique The purpose of this experiment is to become familiar with the specific microbiological technique known as the aseptic technique‚ which is used to avoid contaminating cultures. In this case a pure culture of an unknown organism was introduced to a sterile medium of Phenol Red Glucose Broth Durham. The culture was obtained from a 52-year old male truck driver who is complaining
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Disease: Ergotism Name of Pathogen: Claviceps purpurea Body System affected: Lymphatic/Immune sytstem‚ Digestive‚ Circulatory Bacterial Morphology: They are fungi that resemble small mushrooms in which the perithecia are embedded in the capitate tip. Reservoir: grains (specifically rye) and grass contaminated with ergot Mode of Transmission: Ingestion Symptoms of Illness: Symptoms include constriction of arteries and veins‚ rapid‚ weak pulse‚ precordial distress or pain muscle pain
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Lab Report (Scientific Paper) 2: Bacterial Transformation;DNA Extraction Part I & II:Total Genomic Extraction & Plasmid Extraction;Electrophoresis By:Chris Foster Abstract: We conducted three experiments that included a Bacterial Transformation‚ a two process DNA extraction‚ and a final procedure using gel electrophoresis. The Bacterial Transformation lab was performed to prepare the plasmid into a bacteria and to use that bacteria to amplify the plasmid in order to make large quantities
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Bacterial Transformation Lab Introduction: In this experiment we transformed a strain of E. Coli bacteria without antibiotic resistance with plasmid DNA. This plasmid produces a fluorescent green glow under black light due to the gfp(green fluorescent protein) as well as antibiotic resistance. E. Coli cells will be plated on an agar medium‚ some with and some without the antibiotic ampicillin. Only bacterial cells that contain the plasmid will survive the ampicillin and produce the green glow
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Title: Chapter 7: Bacterial Growth Purpose: The purpose of this experiment is to observe the bacterial growth of Escherichia coli under various conditions. Physical factors and nutritional requirements determine the overall concentration of the bacteria. Along with the use of a spectrophotometer and the technique of serial dilution‚ countable colonies can be obtained. Results are plotted on a semi-log graph in order to observe the different growth curves corresponding to optical density (cell
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Modeling bacterial growth is important in maximizing the efficiencies of biological processes. Although there are many different methods of modeling bacterial growth‚ this experiment focuses on the Monod equations. However‚ in order to use the Monod equations‚ the maximum growth rate and Monod constant must be found. Here we show how the maximum growth rate and Monod constants can be obtained for Escherichia coli using M9 media in a bioreactor at 37 °C and 500 RPM. The maximum growth rate is also
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Bacterial Morphology Part 1: Viewing Prepared Slides of Common Bacterial Shapes Familiarize yourself with each morphological type to use as a comparative tool for the remainder of the activity. Record your observations. Part 2: Disinfecting Your Area to Use Live Organisms: Part 3: Viewing Live Organisms – Wet Mount Preparation There was several amoeba shaped cells that varied in size. There were five somewhat darker areas that were circular in shape. There were also three large
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Study of Bacterial Growth and Resistance Level to Certain Antibiotics INTRODUCTION Escherichia coli—better known as E. coli—is a gram negative‚ rod shaped bacteria. It is relatively harmless‚ but can occasionally cause food poisoning. It can also provide Vitamin K2. It prevents the establishment of pathogenic bacteria‚ and is associated with or found in the intestinal organ. The antibiotic that E. coli is resistant to is Penicillin. Bacillus subtilis—better known as B. subtilis—is known as
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Bacterial Diversity Project John FreesackSection A24 Kim Daffer‚ John Chang September 23‚ 2012 Introduction: Bacteria are everywhere. Some can be seen with the naked eye and some require a microscope but how do we distinguish one kind of bacteria from another? To answer this question‚ we were required to complete three bacterial labs that helped us to understand what microorganisms are and how to identify and classify them. Thus‚ the main purpose of this project is to identify our unknown microorganisms
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