Bacterial Genetics Worksheet
Bacterial Genetics Worksheet
1. Fill in: Transformation Transduction Conjugation a. Transformation _ naked DNA is involved b. Transduction ____faulty head stuffing may occur c. Conjugation_____ involves exconjugants d. Transduction____ adsorption and injection of genetic material e. Transformation__ competent cells required f. Conjugation ____ an F plasmid encodes attachment proteins g. Conjugation _____used to construct the E. coli minute map h. Conjugation_____ a pilus is produced i. Transduction____ lytic cycle entered in response to stress j. Transduction____ may be specialized or general k. Conjugation_____ requires cell to cell contact l. Transduction ____mediated by a virus …show more content…
A plasmid can be maintained extrachromosomally__ or integrate into bacterial chromosome. b. In generalized_ transduction, all bacterial DNA has equal probability of being packaged into progeny phage c. In _ lysogeny __, the phage genome integrates into bacterial genome creating a prophage. d. The F Factor (plasmid)_ contains ~100,000 base pairs and contains 19 genes that encode for proteins involved in pilus synthesis and other functions e. The F factor occasionally integrates randomly into the E. coli chromosome creating a _ Hfr strain _ f. The creation of a knockout mouse uses the process of __ recombination___ to replace DNA with an engineered construct
3. What is the difference between: a. A plasmid and a chromosome
Plasmid: A extra chromosomal which may or may not integrate with the main genome. It gives additional characters to the cell like antibiotic resistance.
Chromosome: The main genome of the cell. It controls all functions of the cell.
b. Transduction and
1. Fill in: Transformation Transduction Conjugation a. Transformation _ naked DNA is involved b. Transduction ____faulty head stuffing may occur c. Conjugation_____ involves exconjugants d. Transduction____ adsorption and injection of genetic material e. Transformation__ competent cells required f. Conjugation ____ an F plasmid encodes attachment proteins g. Conjugation _____used to construct the E. coli minute map h. Conjugation_____ a pilus is produced i. Transduction____ lytic cycle entered in response to stress j. Transduction____ may be specialized or general k. Conjugation_____ requires cell to cell contact l. Transduction ____mediated by a virus …show more content…
A plasmid can be maintained extrachromosomally__ or integrate into bacterial chromosome. b. In generalized_ transduction, all bacterial DNA has equal probability of being packaged into progeny phage c. In _ lysogeny __, the phage genome integrates into bacterial genome creating a prophage. d. The F Factor (plasmid)_ contains ~100,000 base pairs and contains 19 genes that encode for proteins involved in pilus synthesis and other functions e. The F factor occasionally integrates randomly into the E. coli chromosome creating a _ Hfr strain _ f. The creation of a knockout mouse uses the process of __ recombination___ to replace DNA with an engineered construct
3. What is the difference between: a. A plasmid and a chromosome
Plasmid: A extra chromosomal which may or may not integrate with the main genome. It gives additional characters to the cell like antibiotic resistance.
Chromosome: The main genome of the cell. It controls all functions of the cell.
b. Transduction and