"Growth Medium" Essays and Research Papers

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Growth Medium

 MCB 2010C Lab Report: Growth Media 1. State the difference between chemically defined and complex media in your own words. (2) Chemically define is a media that is pure and contains specific compounds from either organic or inorganic. Also, chemically define media is a chemical composition that is known. On the other hand, Complex media is a complex media that is unknown and is usually not a chemically pure. 2. Classify the media used (YEB, NB, GSB and ISB) as either complex or...

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A Lab Report on Microbial Growth

David Kennedy Bio 210 Lab Report 1 10/11/13 Microbial Growth Background Information: This lab was conducted in order to understand basic differences among differential and selective media, while recognizing how each media is used to isolate and identify microorganisms (Wistreich, 2003). The first microorganism analyzed was Staphylococcus epidermidis. This organism is gram-positive, single celled, arranged in grape-like clusters, and cocci in shape (Bukhari, 2004). S. epidermidis is approximately...

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Culture Media

agar plates To distinguish the growth characteristics of microorganisms in various differential, and selective media. Differentiate bacteria based on their ability to hydrolyze starch. Materials: Plates of EMB, Starch and blood agar. Stool sample. Inoculating loop. Bunsen burner. Soil sample. Cotton soap. Skin sample. Gram iodine. Results: Starch agar: Special types of media: Type of medium Medium Appearance of medium and growth Selective and differential Eosin-methylene...

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The Effect of Increasing the Concentration of Dettol Antiseptic Liquid on the Growth of E.Coli

THE EFFECT OF INCREASING THE CONCENTRATION OF DETTOL ANTISEPTIC LIQUID ON THE GROWTH OF E.COLI Background: Escherichia coli are rod shaped, gram negative bacteria often found in the gut of humans and other warm-blooded mammals (Jacques & Ngo, 2004). It is transmitted primarily through faecal contaminated food and water. Most strains of E. coli are not harmful to humans, however some strains are pathogenic and can cause symptoms including diarrhoea (Unknown, 2012). The active ingredient in Dettol...

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Temperature Effects on the Growth of Microorganisms

Temperature effects on the growth of microorganisms Introduction: The purpose of this experiment was to determine the effects that temperature has on three different organisms. Temperature is one of the most important environmental factors affecting growth and survival of microorganisms². The three organisms used where Escherichia coli, Pseudomonas fluorescens, and Bacillus stearothermophilis. Most bacteria grow within a particular temperature range. The minimum growth temperature is the lowest...

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Bacterial Diversity Project

Gram-negative bacteria are positive (forms string) in this test (Leicht 24). The growth on selective medium test can also be used to classify bacteria. Gram-positive bacteria can grow on PEA agar and Gram-negative bacteria can grow on EMB-lactose and Vancomycin agar. Growth on Manitol Salt Agar (MSA) can also be used to classify different types of bacteria. It contains a high concentration of sodium chloride that permits the growth of most bacteria. Catalase and Oxidase test can also be used. The Catalase...

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Anatomy & Physiology Chp 22 Quiz

purpose of the this experiment is to learn to transfer microorganisms to a nutrient medium to promote growth using aseptic techniques. which are a method used to prevent unwanted organisms from contaminating a medium. We will also learn about different types of culture media, including MRS broth and nutrient broth, learn about oxygen and temperature requirements for microbial growth and how to control microbial growth. Procedure: There are four parts to this experiment. The first step is to set...

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Bacteria

using the sterile scissors, cut to the inside to expose the fish organs. Beak the kidney to took the tissue sample by using the inoculation loop. The streak was made on the TSA medium with 2% NaCl. After each streak, the inoculation loop need to heated. The plate was keep for 24 hour at temperature of 30ºC for colony growth. b) Sequestration of bacteria from shrimp Methodology Dissecting set was prepared and soaks it in a beaker that contains 70% alcohol. Took a shrimp and put it in a dissecting...

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Agar plates

McConkey) agar is a culture medium designed to grow Gram-negative bacteria and stain them for lactose fermentation. It contains bile salts (to inhibit most Grampositive bacteria, except Enterococcus and some species of Staphylococcus), crystal violet dye (which also inhibits certain Gram-positive bacteria), neutral red dye (which stains microbes fermenting lactose), lactose and peptone. QUALITY CONTROL Results after 24 hrs at 35º C Organisms ATCC Growth Colour Escherichia coli ...

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Microbiology: Viable Counts

counts the number of colonies produced by a very dilute suspension of bacteria on an agar plate and to observe the differential staining behaviour of the living bacteria. This involves counting the colonies produced by viable cells under favourable growth conditions. Some techniques needed before the viable count, like pour plate method, spread plate method and most probable number method. The viable count is very specidic, as it represents the number of colony forming units (/g) or (/ml) of the sample...

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