RPT Extraction of DNA

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Extraction of DNA
Hands-On Labs, Inc. Version 42-0056-00-01
Lab Report Assistant
This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable file which can be sent to an instructor.
Observations
Questions
Describe what the DNA from the split peas looked like?
It looks white, stringy, tough and I was surprised by how simple it is to extract the DNA from a food item.
B. Were your experimental results what you expected to see? Specifically, was there more or less DNA than you expected?
No, I expected more DNA. I also didn’t expect to see it form on the top of the tube so easily.
C. What was the purpose of adding the detergent to the experiment? If the detergent was not used, do you think the experiment results would have changed? Explain your answer.
The detergent is used to break down the lipids and proteins in the cell and it breaks the bonds that hold the cell membrane together. I don’t think it would work as well without the detergent and the results may have been more difficult to see.
D. If you used 5% isopropyl alcohol instead of 91% isopropyl alcohol, do you think the experiment results would have changed? Explain your reasoning.
Yes the results would have changed. The higher percentage of alcohol works better to precipitate the reaction. It renders the insolubility of the DNA in the pea to an aqueous

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