top-rated free essay

osmosis in onion cell

By arembo7 Dec 04, 2013 743 Words
Date: 28/11/13

A living plant cell will shrinks or swells depending on the solute concentration of the cell in relation to the solute concentration of the fluid surrounding the cell (1). It follows that water will move from a region of high water concentration to a region of low water concentration therefore, if a cell is placed in a hypertonic solution water will move from the cell into the solution until the cell shrinks(1). Further water loss causes can cause the cells protoplasm to peel away from the cell wall leaving a gap between the cell wall and the cell membrane, a process called plasmolysis(1) However if the cell is placed in a hypotonic solution, water will move from the solution into the cell and cause the cell to burst (1). A cell in an isotonic solution neither swells nor shrinks because there is no concentration gradient for water across the cell membrane (1) The objective of this experiment is to find out the effect distilled water, 3% sodium chloride solution and 5% sodium chloride solution have on onion epidermal cells. From the above literature, its hypothesised that the cells in distilled water should maintain their shape, cells in 3% sodium chloride should shrink and the cells in 5% sodium chloride should experience more shrinking than that of the 3% solution. The onion epidermal cell is transparent with a simple structure so it is a suitable cell for studying the effect of water loss on cells (2) Some specimens can be viewed directly underneath the microscope but putting a drop of water on the specimen can improve how the structures appear under microscope and also prevent the specimen from drying out on the slide (2). Adding water to the specimen is called wet mount. The liquid used in wet mount fills the space between the slides to support the specimen and also allows light to pass through easily (3). The liquid is usually water can also be a stain.

Stains are used in microscopy to highlight structures in biological structures for viewing under microscopes (3). There are many types of stains each is used for its own benefits, iodine, eosin are some of the common stains used in the microscopy (3). Iodine is used as a cell stain because it’s absorbed by cell organelles which are mostly transparent and turn them coloured making them more visible under the microscope (3). It was found that the cells which were placed in 3% solution increased in size contrary to the hypothesis. The cells placed in 5% solution shrank and cells in placed in normal saline kept their normal shape.

Glass slide
Cover slip
Onion tissue
Light miscrope
Sodium chloride (NaCl)
Distilled water
Measuring tube
Bunsen tube
Blotting paper
Stage micrometer

Lay down three different slides labelling the three different solution you intend to use. Peel a thin layer of tissues (epidermis) using the forceps (tweezers) from the whole onion. Put the epidermis on the center of each of the slide displayed and labelled. Drop a few drops of each of the labelled solutions onto the onion membrane, making sure the tissues is flattened and not wrinkled on the slide. Wait for 3 minutes before putting a cover slip on the slide. Put the cover slip on the stained tissue and the different solutions involve. Gently tap out any air bubbles and try to soak out any extra solution around the covered tissues. Adjust the diaphragm so that the right amount of light is shining on the slide stage. Put the slide onto the microscope stage and fix with the stage clips Observe the tissues under the microscope under low, medium and high powers. Also making sketches, writing down observation. Making sure of an eye piece graticule and stage micrometer scale to measure cells and units. Be sure to write what magnification for each sketch.

Make sure you label any organelles you see. Cell wall, membrane, nucleus and cytoplasm Calculate magnification of drawings and actual sizes of specimens

The eye piece graticule remains constant no matter what magnification the epidermis are looked at.




1. Sperelakis, Nicholas (2011). Cell Physiology Source Book: Essentials of Membrane Biophysics. Academic Press. p. 288. ISBN 978-0-12-387738-3. 2. Horobin RW, Kiernan JA (2002) Conn's Biological Stains. A Handbook of Dyes Stains and Fluorochromes for Use in Biology and Medicine. 10th ed. Oxford: BIOS. ISBN 1-85996-099-5 3. Clark G (1981) Staining Procedures, 4th ed., Baltimore: Williams & Wilkins, p. 412, ISBN 0683017071.

Cite This Document

Related Documents

  • Osmosis: Sodium Chloride and Onion Cells

    ...Osmosis/Plasmolysis Lab Name: Problem: How do solutions of various salt concentrations influence osmosis in relation to an onion cell? Materials: (per student group): red onion epidermis forceps, dropper distilled water NaCl solution paper towels microscope slide cover slip Procedure 1. Make a wet m...

    Read More
  • Osmosis in Onion Cell

    ...sucrose concentration influenced osmosis in relation to three onion cells and the impact on the cells structure. A small square of a red onion skin (membrane) was observed under a microscope at high power (X40) magnification. The observation showed a large number of onion cells. The structure of one onion cell had a general rectangular shap...

    Read More
  • Osmosis in Red Onion Cells

    ...Osmosis in Red Onion Cells By: Youssef Gharib Brief Description of Osmosis in Red Onion cells: Osmosis is the diffusion of water from an area of low concentration to an area of high concentration across a semi-permeable membrane. The purpose of this lab is to compare the three different types of solutions affect on the relative size of the ...

    Read More
  • Osmosis: Cell and Epidermal Cells

    ...36 osmosis in living plant cells Backround If a plant cell is in contact with a HYPERTONIC solution, that is a solution that has a highersolute concentration than the cell contents, water leaves the cell by osmosis via the cell membrane. Water is lost first from the cytoplasm and then the sap vacuole through the tonoplast. The PROTOPL...

    Read More
  • Onion Cell

    ...Unit 1: fundamentals of science. Title An investigation of an onion cell using a light microscope. Aim: The aim of this investigation is to identify the cells within an onion skin using a light microscope. An onion cell is a plant cell which through the light microscope, it should outline the cell wall, cell membrane and the nucleus. Int...

    Read More
  • Osmosis and Cell

    ...________________________ Student Exploration: Osmosis Vocabulary: cell membrane, concentration, diffusion, dynamic equilibrium, osmosis, semipermeable membrane, solute, solvent Prior Knowledge Questions (Do these BEFORE using the Gizmo.) 1. Suppose you were trapped on a desert island with no sources of fresh water. Should you d...

    Read More
  • Osmosis: Cell Wall and Cover Slip

    ...the level of cells. Objectives: 1. To demonstrate the principle of Osmosis and to apply it with actual life situations. Materials and Methods: • Materials:  Compound microscope  Glass Slide  Cover Slip  Tissue Paper  Blade  Rhoeo discolor Leaves  Salt Solution • Procedures:  Place a drop of d...

    Read More
  • osmosis

    ...Trial 5 No. of cells No. of cells plasmolysis No of cells No of cells plasmolysis No of cells No of cells plasmolysis No of cells No of cells plasmolysis No of cells No of cells plasmolysis 0.3 43 7 39 5 40 9 38 6 34 4 0.4 40 6 40 8 40 6 38 7 40 8 0.5 42 16 44 17 42 13 43 18 42 17 0.6 42 22 42 ...

    Read More

Discover the Best Free Essays on StudyMode

Conquer writer's block once and for all.

High Quality Essays

Our library contains thousands of carefully selected free research papers and essays.

Popular Topics

No matter the topic you're researching, chances are we have it covered.