ISSN: 2229-791X www.researchinbiotechnology.com Regular Article
Isolation and identification of a new Bacillus strain for amylase production
Yasser Bakri*1, Hassan Ammouneh1, Samir El-Khouri1, Muhanad
Harba1 and Philippe Thonart2
of Molecular Biology and Biotechnology, AECS, Damascus, Syria, P.O.Box 6091
Wallon de Biologie Industrielle, Unité de Bio-industrie, Faculté de AgroBiotechnologie de Gembloux, Université de Liège, Passage des Déportes 2, B-5030
*Corresponding Author E-mail address: email@example.com
The objective of the study was to isolate amylase producing bacteria from Syrian soil samples. Amylolytic Bacillus sp were isolated on starch agar medium. The Bacillus
SY134D was the best amylase producing isolate among two hundred and ten isolates showed the amylolytic activity. The closest phylogenetic neighbours according to 16S gene sequence, the Bacillus SY134D isolate was identified as Bacillus subtilis. The optimum temperature for amylase production was found to be 37°C. Amylase production occurred at pH 3.0-9.0 with a maximum at pH 5.0. The best enzyme activity was observed at pH 6.0 and temperature 70°C.
Key Words: Bacillus sp; solid culture; α-amylase.
Alpha amylase (EC188.8.131.52, 1,4alpha-D-glucan-glucanohydrolase) is an extracellular enzyme, which is involved in the starch processing industries where it breaks starch down into simple sugar constituents (Reddy et al., 2003; Asgher et al., 2007). Alpha amylases have extensive applications in starch processing, brewing and sugar production (Leveque et al., 2000), in textile industries and in detergent manufacturing processes (Asgher et al.,
2007). Interestingly, the first enzyme produced industrially was an amylase from a fungal source in 1894, which was used as a pharmaceutical aid for the treatment of digestive disorders (Pandey et al., 2000).
Amylases are among the
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