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How Does Temperature And Ph Affect The Rate Of An Enzyme Lab

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How Does Temperature And Ph Affect The Rate Of An Enzyme Lab
Abstract:
This lab tests how temperature and pH affect how enzymes will function. The lab showed that temperature will denature an enzyme when past its optimal working temperature and won't denature in cold temperatures, but have slowed molecular activity. pH will also have an affect on an enzymes efficiency, when out of optimal pH the enzyme will not function as it is supposed to and if to far out of the optimal pH the enzyme will change shape and no longer work. Enzymes also showed to be reusable after the experiment was complete.
Introduction:
This lab tested how enzymes are able to affect the rate of chemical reactions and how the rate of which an enzyme works in different conditions. The conditions the enzymes were tested in included
…show more content…
This hypothesis should have been proven true with the experiment, but due to some error the iced liver had the greatest rate of O2 production. Things that could have affected the rate of reaction could include: the iced liver being left in room temperature conditions too long before beginning the experiment, accidental added motion from moving the test tube during the reaction causing molecular collision to occur more frequently increasing the rate of the reaction. Then the hypothesis regarding the pH experiment stated, the enzymes found in pH 3 and pH 10 will have little activity and 7 should show the greatest activity, because our bodies function around a pH of 7. This was proven true, because during experiment the pH of 7 performed the best. The final hypothesis stated, the used iced liver brought back to room temperature will have a similar O2 production to the room temperature liver because enzymes are reusable. This was proven to be true, because the formerly iced used liver was able to produce O2 at a similar as the unused

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