Biology ia

Topics: Agar plate, Petri dish, Growth medium Pages: 11 (1370 words) Published: March 24, 2014
002141-0012

Nuba Jackson IB Biology
Microbiology IA
How effective is Lysol in the reduction of bacterial growth compared to Pinesol in reduction of E. Coli growth in agar at room temperature? 

Background Information:

Pinesol and Lysol are both common household disinfectants that make very big commercial claims; both claim to kill 99.9 percent of bacteria. Lysol contains Phenol which is a highly volatile organic compound. Phenol is a carbolic acid found in some household cleaning supplies. Phenol is very toxic and because of this it is designed to kill bacteria. The phenol within the Lysol causes the environment where the bacteria thrive to be uninhabitable. Pinesol has several other organic compounds in it as well but none as volatile as Phenol, yet some consumers say that Pinesol is just as useful as Lysol. Pinesol contains allkyl and isophryll acohol which are both do not disrupt the environment of the bacteria as effectively.

variables

Measurement

range

Independent

Lysol and pinesol

Concentration

2-3 milliliters

dependant

Bacterial growth

colonies

5-7 Colonies

Controls

Uses

how

Agar plate

The same agar allows this specific type of bacteria to grow; and adds consistency to environment.

Use same type of agar plates.

moisture

Allows researcher to know how bacteria reacts with condensations

Use same incubator for storage

temperature

Adds consistency to environment and gives bacteria optimum environment for growth.

Use same incubator for storage

Type of bacteria

Adds consistency for experiment and is a bacterium

Use same type of bacteria (E. Coli)

Hypothesis:

I think the Phenol in the Lysol will cause the most significant reduction in bacterial growth, because the Phenol is a volatile organic compound capable of bacterial reduction in extensive amounts.

Materials:

1 bottle of Lysol

1 bottle of Pinesol

2 graduated cylinders

2 blood agar plates

sterilized cottonswabs

2 incubator rods

2 petri dishes

Procedure:

1)
Wash your hands

2)
Label the top of the container with your initials and name of household cleaner that is going to be used on the Streptococcus Baccillius.
3)
Begin by sterilizing the petri dishes. Accomplish by placing them in a bath of boiling water and allowing to dry upside down on a sanitized drying rack or lab work bench. If the Petri dishes are still enclosed in the original packaging, leave them until ready to use. For 500 milliliters (ml) of agar mixture, you can fill 25 average-sized petri dishes.

4)
Prepare agar powder by placing it in the microwave with the appropriate amount of water. The label should provide specific directions although 6.9 grams to 500 ml of water (or just a little more than two cups) is standard. For agar tablets, 10 tablets dissolved in two cups of water is standard.

5)
Treat bottled agar formula carefully by loosening the bottle cap (but not removing) before placing in the microwave to warm and soften into a liquid state. Sterilize the bottle after microwaving by placing the neck of the bottle over an open flame a few times to ensure any airborne germs are eradicated before proceeding to plate making.

6)
Place the petri dishes the right way up and crack but do not remove the lids. This is to prepare them for individual handling when the other hand is holding the agar mixture in a suitable pouring vessel such as a glass pitcher.

7)
Gently lift the lid of the petri dish with one hand and hover the lid immediately over the bottom dish to remove any chance of airborne germs entering.
8)
Pour an amount of agar liquid into each dish as you hover the lid as mentioned in the previous...
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