Purpose: Unknown mixtures will be separated by means of chromatography in which the mixture will be passed in a solution through a medium leaving behind components of the mixture at different rates‚ therefore‚ different spots on the absorbing substance. This will help determine the identity of unknown mixtures. The spot colors on the strip of filter paper and the Rf values of the unknown samples will be compared to those of known samples. To find the position of the spots on the strip of paper‚ we
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Identification and Diagnosis of Unknown Enteric Bacteria in an Infected Patient Using an Enteropluritube Brooke Addario 2/16/2015 BIO 488C - Section J TA – Jacob Karsten Introduction: Enteric bacteria are members of the Enterobacteriaceae family of microorganisms. These gram negative‚ rod shaped‚ facultative anaerobes are found in the intestinal tracts of humans and animals. Enteric bacteria can be pathogenic; however‚ they are also a normal part of human bacterial flora and are therefore
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Macromolecules Lab Purpose: to determine a method of testing for macromolecules. Materials: Knowns: Unknown: Test Solutions: Glucose solution Unknown solution Iodine solution Gelatin solution Benedict’s solution Starch solution Biuret solution Oil Brown paper Water Procedure: 1. Create a data table. 2. Label 5 test tubes with known solutions. 3. Add 10-20 drops of each known solution to respective test tubes‚ do not mix pipets! 4. Add 3-5 drops of
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Unknown Bacterium #22 Purpose The purpose of this experiment was to find the identity two unknown bacteria in the given test tube. Materials •Bacterial Loop •Bunsen Burner • Petri Dish with agar •Crystal Violet •Gram’s Iodine •95% Ethanol •Safrin •Glass Slides •Microscope •Unknown bacteria in test tube Procedure On the first day‚ one plate was streaked qualitatively and left it in the
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9/20/2013 Methicillin-Resistant Staphylococcus aureus Methicillin-Resistant Staphylococcus aureus also known as MRSA is a bacteria frequently found on the skin of generally healthy people. MRSA is usually spread through skin to skin contact or contact with an object containing the bacteria. Most of the time you may be carrying the bacteria but you are not ill. Mostly people with weak immune systems and have been in health care centers
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Staph infections are caused by a staphylococcus bacterium‚ Staphylococcus aureus. S. aureus is a purple‚ cocci shaped cell with its morphological arrangements in grape-like clusters‚ it is determined by a bacteriological stain‚ known as the Gram stain. This determines it to be a gram positive bacterium. When grown on bacteriological media‚ S. aureus appears as a large white to golden colony. It is not very fastidious but it grows well‚ either aerobically or under anaerobic conditions and produces
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| Gram Negative Unknown | Biology 3444-006 | | Lena Wallace | 11/7/2011 | | Abstract: The purpose of this lab was to identify an unknown bacteria culture using differential tests. The identification of the unknown culture was accomplished by identifying the bacteria based on its specific metabolic characteristics and morphology. It is suggested that culture 11 is a sample of Enterobacter aerogenes. Introduction: This experiment was centered on metabolic and biochemical testing
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Microbiology UNKNOWN LAB REPORT Kateryna Petrakova INTRODUCTION Microorganisms cause a great amount of diseases. For healthcare providers it is very important to be aware of what organisms are pathogenic and cause a disease and‚ therefore‚ to find an appropriate treatment. Different microorganisms require various environments in order to replicate and to become dangerous for a person’s health. An integral part of any medical treatment is to be able to recognize and
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Molecular Characterization of Methicillin-Resistant Staphylococcus aureus (MRSA) Nasal Colonization and Infection Isolates in a Veterans Affairs Hospital Staphylococcus aureus (S. aureus) belongs to the genus Staphylococcus. It is Gram-positive‚ cocci shaped anaerobic bacteria that forms clusters and produces catalase‚ which catalyzes hydrogen peroxide to water and oxygen. It contains a cell wall structure (including peptidoglycan and teichoic acid) and a low G + C content of DNA between 30-40 mol
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Traditional Analysis of Unknown Colony Morphology Color- light yellow pigmentation Form- circular Elevation- convex Margin- entire [staphylococci] DNase Test This test is used to detect if the bacteria contains any deoxyribonuclease activity. Because no color change was observed from blue to clear my unknown bacteria displayed a negative result. Blood agar plate This test is used to detect the hemolytic activity in the bacteria. A darkish green color on the media around the
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