Unknown Macromolecules Lab Report

Macromolecules Lab
Purpose: to determine a method of testing for macromolecules.
Materials:
Knowns: Unknown: Test Solutions:
Glucose solution Unknown solution Iodine solution
Gelatin solution Benedict’s solution
Starch solution Biuret solution
Oil Brown paper
Water

Procedure:
1. Create a data table.
2. Label 5 test tubes with known solutions.
3. Add 10-20 drops of each known solution to respective test tubes, do not mix pipets!
4. Add 3-5 drops of a test solution to each test tube.
5. Record the results (a color change indicates a positive result).
6. Clean out each test tube and repeat steps 3-5 for remaining test solutions.
7. For the brown paper test, put one drop of each known solution on the paper, record results.
8. Using your data, determine which tests are used to detect which macromolecules. Write down your observations about each test.
9. Test the unknown to determine contents, record all results.
10. Clean all test tubes, remove all labels, clean up lab space, and return all materials to the appropriate locations.

Data:
Changes of Test Solutions Mixed With Known and Unknown Solutions

Iodine Solution
Gelatin solution is a form of protein, it also changed color to purple when Benedict’s and Binret solution was mixed with it. Also its drop stayed on brown paper too. Starch solution belongs to carbohydrates; it changed color with iodine and Benedict’s solution, but dissolved on brown paper. Oil a form of lipids, not only it dissolved with brown paper but it also changed color with Benedict’s solution. Meanwhile, water will be the control group which performs opposite of all the other macromolecules. Therefore, the unknown solution is a mixture of all the other macromolecules because it has same reactions as every