microbiology final exam unknown bacteria report example
Microbiology
UNKNOWN LAB REPORT
Kateryna Petrakova
INTRODUCTION
Microorganisms cause a great amount of diseases. For healthcare providers it is very important to be aware of what organisms are pathogenic and cause a disease and, therefore, to find an appropriate treatment. Different microorganisms require various environments in order to replicate and to become dangerous for a person’s health. An integral part of any medical treatment is to be able to recognize and identify a specific bacterium that can create potentially a big problem for a patient. Current study aims to identify unknown microorganisms using all the necessary test and techniques, learnt during microbiology laboratory classes.
MATERIALS AND METHODS:
A tube labeled 117 was given out by the lab instructor. The tube contained two different types of bacterium. In order to identify what bacterium were present in the tube isolating techniques were performed first. Using lab manual recommendations the first procedure was isolating pure cultures. Using inoculating loop and dry heat technique, isolation streak was performed and nutrient agar plates were to be incubated in a room temperature for the next 48 hours. Nutrient Agar plate was used for isolation streak technique in order to see two types of bacterium growing in a room temperature.
After incubating for 48 hours Nutrient agar plates were examined for bacterial growth of two different colonies. On a Nutrient agar plate two different cultures were observed. In order to proceed identification, those cultures were isolated as separate organisms, using inoculating streak technique and incubated until the next class period. The expected result was supposed to show Gram positive and Gram negative bacterium growing on a separate media.
Having checked two Nutrient plates, signs of visible growth of two different bacterium were present. Due to the fact, that one type of bacteria didn’t appear to be fully
UNKNOWN LAB REPORT
Kateryna Petrakova
INTRODUCTION
Microorganisms cause a great amount of diseases. For healthcare providers it is very important to be aware of what organisms are pathogenic and cause a disease and, therefore, to find an appropriate treatment. Different microorganisms require various environments in order to replicate and to become dangerous for a person’s health. An integral part of any medical treatment is to be able to recognize and identify a specific bacterium that can create potentially a big problem for a patient. Current study aims to identify unknown microorganisms using all the necessary test and techniques, learnt during microbiology laboratory classes.
MATERIALS AND METHODS:
A tube labeled 117 was given out by the lab instructor. The tube contained two different types of bacterium. In order to identify what bacterium were present in the tube isolating techniques were performed first. Using lab manual recommendations the first procedure was isolating pure cultures. Using inoculating loop and dry heat technique, isolation streak was performed and nutrient agar plates were to be incubated in a room temperature for the next 48 hours. Nutrient Agar plate was used for isolation streak technique in order to see two types of bacterium growing in a room temperature.
After incubating for 48 hours Nutrient agar plates were examined for bacterial growth of two different colonies. On a Nutrient agar plate two different cultures were observed. In order to proceed identification, those cultures were isolated as separate organisms, using inoculating streak technique and incubated until the next class period. The expected result was supposed to show Gram positive and Gram negative bacterium growing on a separate media.
Having checked two Nutrient plates, signs of visible growth of two different bacterium were present. Due to the fact, that one type of bacteria didn’t appear to be fully
References: “Methicillin-Resistant Staphylococcus Aureus (MRSA)-Overview.” WebMD. WebMD, n.d. Web. 29 Apr. 2013. McDonald, Virginia, Mary Thoele, Bill Salsgiver, and Susie Gero. Lab Manual for General Microbiology. N.p.: n.d. Print. 101010 0411.