to determine two unknown samples (X and Y) by comparing results to the known standard drugs‚ of which there was five‚ using a specific TLC method. The same technique was used for the determination of the unknown; however‚ the solution used for each method was slightly different. Furthermore‚ the mobile phase in each method was of different concentration with the addition of ethylacetate in Solvent system A. The distance moved by the spot could not solely determine the unknown sample and so‚ the
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Title: Bradford Assay: Creating a Standard Curve and Determining Unknown Protein Concentrations Introduction: In biochemistry the ability to determine the quantity of a certain substance in a solution is extremely important. Often protein concentration within a biological organism is essential in determining the function or condition of that organism. If the protein concentration in an environment is increased or decreased it can have detrimental side effects. If there is a decrease in hemoglobin
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world: “The Industrial Revolution was the transition to new manufacturing processes in the period from about 1760 to sometime between 1820 and 1840.” (Unknown) There was many new developments: “This transition
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Macromolecules BCM261/BCM262 Anonymous Student #2 Aim The purpose of this lab was to use the scientific method to identify an unknown solution‚ based on the reactions (i.e. color changes) of known solutions with indicator solutions. These known solutions contained different types of macromolecules. Each type of micromolecule reacted with at least one indicator solution in a unique way‚ which allowed us to identify the macromolecule based upon the presence or
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and purify the unknown D liquid and solid by using its acidic and basic characteristics in a chemically active extraction then to identify the unknowns by analyzing the physical properties on IR and NMR spectrums. The neutral solid was purified through recrystallization and the melting point range was determined to be 47.5oC to 48.9oC. The basic liquid was purified with simple distillation and the boiling point of 135oC was concluded. Through the analysis of IR spectrum of each unknown the structures
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Introduction The purpose of the experiment was to oxidize and identify an unknown alcohol using hypochlorite‚ or household bleach. During the experiment‚ the bleach oxidized the secondary unknown alcohol into a liquid ketone which was distilled or boiled to find the boiling point and then identified using a chart of unknown identities and boiling points. The oxidation of the unknown alcohol was done through the mixing of the unknown alcohol‚ glacial acetic acid‚ and bleach. Through the procedure of oxidation
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Yvan Mamboukuate 10/09/2013 BIO 101 Lab Report Biological Molecules Introduction: In this experiment‚ simple chemical tests will be performed to identify the presence of various types of biological molecules. These tests take advantage of the fact that different types of molecules have different functional groups and‚ therefor‚ different chemical properties. Thus‚ they will react to test reagents (also called indicators) differently. Materials: Biuret‚ Sudan III‚ Iodine‚ Benedict‚ water
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An Introduction to Qualitative Analysis Purpose: Tests the ions of Mg2+‚ Ca2+‚ Sr2+‚ and Ba2+‚ and identify them separately. By using these observations‚ identify the unknown ion. Then tests the ions of SO42-‚ CO32-‚ Cl-‚ and I-‚identify them separately‚ and use the observation to identify the unknown. Procedure Part I - Qualitative Analysis of Group 2 Elements Mix 0.02M K2CrO4 with each Mg(NO3)2‚ Ca(NO3)2‚ Sr(NO3)2 and Ba(NO3)2 together. Secondly‚ mix 0.1M (NH4)2C2O4 instead
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Nick Boyea Billy Lee 3/9/11 Molar Mass by Freezing Point Depression Overview The purpose of this lab is to measure the freezing point depression of a solution of an unknown substance and BHT to determine the molar mass of the unknown substance. Summary of Lab Procedure If not already completed‚ crush a small amount of BHT and pack it into a capillary tube. Use a small rubber band to clamp the capillary tube to the thermometer‚ and fasten the thermometer to a ring stand. Fill a Thiele tube
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Proteus mirabilis The genus Proteus‚ according to Bergey’s Manual of Determinative Bacteriology‚ is a group of straight rod Gram negative bacteria around 0.4-0.8 μm in diameter and 1-3 μm in length. In the microscopic test done‚ it was seen that the unknown culture contained rods that are on the shorter side‚ thus was identified as bacteria that appear to be coccobacilli. Because of the presence of peritrichous flagella‚ species included this genus are motile and most strains swarm in periodic cycles
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