Abstract Carbohydrates are important in metabolic processes for everyday physical and chemical actions. The carbohydrate‚ glucose‚ is a key component in generating adenosine triphosphate‚ also known as ATP. In order to analyze unknown glucose levels‚ a DNS assay was performed. By using 2-hydroxy-3‚5-dinitrobenzoic acid to oxidize the aldehyde group on the carbohydrate‚ the reducing end of glucose increases in absorbance of 540 nm. Using a UV spectrophotometer‚ the concentration was calculated
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Lehman’s Operational Organic Chemistry Lab Manual. (Pgs. 31-32). 2) See page 36 of Lehman’s Operational Organic Chemistry Lab Manual Results To find out the identification of the unknown‚ 3 separate trials had to be performed to the unknown‚ some standards and a mixture of 50/50 of the unknown and the standard‚ or in the case of trial 3‚ both standards of aspirin & acetylsalicylic acid were combined in a 50/50 mixture. (See Table 1 below). * = Standards. In each trial each separate
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Determination of Ka of Weak Acids Purpose: The purpose of this lab is to find the strength of weak acids by determining the equilibrium constants for their ionization reactions in water. Is to use their measured pH values to calculate the pKa for the two unknown weak acids thus determining their identities. Hypothesis: If we neutralize a solution that contains a weak acid by adding a strong base to the solution‚ then the ions will be isolated and thus finding the concentrations of each species by measuring
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Objective:- The aim of this experiment was to find the molar mass of unknown volatile liquid under known temperature and pressure. Introduction Molar mass or gram molar mass is the mass of the substance under consideration without distinguishing whether a substance is an element ‚ a molecule or an ionic compound. These elements‚ molecules or ionic compound could be solid gas or liquid. The molar mass of gas or vapor could be measured if the gases obey the law of Ideal Gas . The mass of
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ingredients. During the experiment‚ four different substances (cooking ingredients) were tested on‚ plus the substance on Smeddy‚ with three different liquids. The five substances tested on were baking powder‚ baking soda‚ cornstarch‚ sugar‚ and Unknown A (the substance Smeddy had). The liquids used to analyze the chemical and physical changes on the substances were water (H2O)‚ vinegar‚ and an iodine solution. In a spot plate‚ a small amount of each substance was put into three separate spots and
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in separating components of a mixture is paper chromatography. We use paper chromatography to separate mixtures and isolate there components to collect them individually. In this experiment‚ we will use acid- base indicators to help determine the unknown mixture. Acid- base indicators will change in color when the presence of pHs rise and fall when acids or bases are added to a solution. After setting strips of paper into a flask with the eluding solvent and waiting 45-60 minutes‚ we will set the
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with a known mole can be used to find characteristics such as the concentration of a reaction in a reaction. Through the experiment‚ the concentration of NaOH‚ and mystery of the unknown acid # 25 was accomplished. In both parts A and B of the experiment‚ a weak acid-strong base reaction occurred. Results indicate that unknown acid # 25 is oxalic acid which is a weak acid. When oxalic acid dissolves in water ‚ a few of its molecules will ionize in water‚ and a small amount of hydronium ions are produced
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pattern for each dilution step (Table 3.1). Although the pH of B4 was less than the pH levels of B2 and B3‚ it was greater than the pH of full strength 1 M NaOH (Table 3.1). The unknown solutions B and F were in a lower pH range of 3.28 and 3.65‚ while unknown solution A had a slightly higher pH level of 4.78 (Table 3.1). Unknown solutions D‚ E‚ and C had an increasing pattern of higher pH levels 10.78‚ 11.41and 12.71 (Table 3.1)
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Ben Quinones AP Chem Mr. Perry 25 January 2013 Molar Mass by Freezing Point Depression Conclusion: My Lab unsuccessfully found the molar mass of the unknown given to us in the lab. We were tasked with finding the molar mass of an unknown compound using the colligative property of Freezing Point Depression. The way we did this was first finding the temperature at which ice crystals form for just regular BHT. Luckily thanks to our TA we were given temperature probes so we could pinpoint exact
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Experiment 3 Colligative Properties Freezing-Point Depression and Molar Mass By‚ Andrew Klingsporn Joby J. Chem 212 Dr. Chandana Meegoda 2/11/2009 Purpose The purpose of this experiment is to determine the Molar Mass of an unknown substance using its freezing point depression. Introduction There are two types of mixtures; homogenous and heterogeneous. Homogenous mixtures have components that are uniformly mixed‚ while heterogeneous mixtures do not. A solution is a homogenous mixture
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