Tanti Lim Thurs AM Unknown Project I. Introduction The purpose to this lab was to isolate and identify two unknown bacteria from a mixed culture provided to us. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed‚ provided us with some key information about the unknown microbes in question . The identification of unknown bacteria is a time honored part of microbiology courses. It will challenge
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symbiotic relationship with its host‚ and for this reason it rarely causes diseases and is usually considered nonpathogenic (Avdic‚ Habes‚ and Avdic 3885). Recently though‚ the microorganism is becoming the common cause of nosocomial infections. In “Microbiology: with diseases by taxonomy”‚ Richard Bauman defines a nosocomial infection as “a disease acquired in a healthcare setting.” These infections are often found with implants and plastic items that have inserted into the body‚ such as catheters‚ pacemakers
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Gbengon‚Sennie Microbiology Lab Case study # 5 November 1‚2012 1. What evidence suggests that the young graduate student has contracted what disease? Ans: The signs and symptoms the young graduate student presented‚ show evidence of Lyme disease. 2. What is the environmental pathway for the vector of this disease? Ans: The environmental pathway for the vector of this disease would be a forested habitat. The black-legged tick or the deer tick is the principle vector of this disease (Lyme
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Discussion The possible identity of the unknown organisms in the mixed culture was limited to bacteria that we had worked with previously in lab. Initially a Gram stain was conducted in order to distinguish the unknown bacterium as a Gram-positive and/or a Gram-negative organism (Lancaster and Bennett‚ 2012; Kellenberger‚ 2001). Based upon the results‚ both Gram-negative and Gram-positive bacteria were observed in the unknown mixed culture (Table 1 and Table 2; Kellenberger‚ 2001). In order
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this lab was to isolate and identify two unknown bacteria from a mixed culture provided to us by our instructor. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed‚ provided us with some key information about the unknown microbes in question and how the bacteria function. Materials and Methods Over a two week period‚ eight prepared types of test media were provided to identify the assigned unknown mixed
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Gram Negative Unknown Lab Report Abstract The Unknown Gram Negative bacterium inoculated in a Tryptic Soy broth medium was randomly selected from a group of other unknowns. In order to identify this unknown the seven different types of biochemical tests will be conducted on this unknown bacterium to identify it out of 6 possible bacteria; Escherichia coli(E. coli)‚ Enterobacter aerogenes (E.aerogenes)‚ Klebsiella pneumoniae (K.pneumoniae)‚ Proteus mirabilis(P.miranilis)‚ Pseudomonas aeruginosa(P
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our skills we have learned in the lab to identify two unknown organisms. PROCEDURE I was given unknown #76. I performed the streak plating method with my unknown organisms. To perform this exercise I needed a TSA plate and labeled the bottom of it with my name‚ group number and organism. I also divided the bottom into three sections. After that‚ I sterilized my inoculating loop using the bunsen burner flame. After letting the loop cool‚ I reached into my unknown test tube with my loop and grabbed
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1. Title: Double unknown identification of a mixed culture of Gram-negative and Gram-positive bacteria 2. Author: Nick Fiore‚ University of Kansas‚ Biology 402‚ Fall 2014 3:00pm room 6040 3. Abstract: The purpose of this experiment was to isolate two unknown bacteria and perform a series of selective and differential tests to correctly identify each. After the bacteria was isolated a series of differential and selective tests following the dichotomous key attached were used to identify each bacteria
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Unit 6 Lab Report 1. Objective: To find the percent composition of water in an unknown hydrate. 2. Materials: Goggles‚ apron‚ evaporating dish‚ hot plate‚ and stirring rod 3. Procedure: get the mass of a evaporating dish‚ put a sample of the hydrate onto the evaporating dish and mass the dish with hydrate. Put the dish with hydrate on the hot plate on high heat. Break up any clumps that form during the heating process. Observe any color
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Unknown Bacterium #22 Purpose The purpose of this experiment was to find the identity two unknown bacteria in the given test tube. Materials •Bacterial Loop •Bunsen Burner • Petri Dish with agar •Crystal Violet •Gram’s Iodine •95% Ethanol •Safrin •Glass Slides •Microscope •Unknown bacteria in test tube Procedure On the first day‚ one plate was streaked qualitatively and left it in the
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