The first test conducted on unknown bacteria 32 was the Gram stain. From this stain‚ unknown 32 was found to be a Gram-positive cocci. This test eliminated all possible Gram-negative bacteria‚ Gram-positive rods and Gram-positive spirillium. Next‚ the endospore test determined whether or not the Gram-positive bacteria contained endospores. With the use of malachite green‚ steam‚ and safranin it was found that unknown bacteria 32 did not contain endospores. This eliminated Gram-positive cocci Sporosarcina
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our skills we have learned in the lab to identify two unknown organisms. PROCEDURE I was given unknown #76. I performed the streak plating method with my unknown organisms. To perform this exercise I needed a TSA plate and labeled the bottom of it with my name‚ group number and organism. I also divided the bottom into three sections. After that‚ I sterilized my inoculating loop using the bunsen burner flame. After letting the loop cool‚ I reached into my unknown test tube with my loop and grabbed
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Unknown Bacterium #22 Purpose The purpose of this experiment was to find the identity two unknown bacteria in the given test tube. Materials •Bacterial Loop •Bunsen Burner • Petri Dish with agar •Crystal Violet •Gram’s Iodine •95% Ethanol •Safrin •Glass Slides •Microscope •Unknown bacteria in test tube Procedure On the first day‚ one plate was streaked qualitatively and left it in the
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Abstract The primary focus of this lab was on microscopy and simple stains. Crystal violet and Carbol fuchsine‚ simple staining components‚ were used to stain the slide in order to see the different microbes in order to determine their cellular shape and identify unknown ones by comparing. Introduction Bacterial cells are usually colorless because cytoplasm‚ for the most part‚ is transparent. Since the bacteria are colorless‚ it is almost essential to add a stain to make the bacteria more visible
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Microbiology Final exam Study reference 1. Gram Stain * Verify if bacteria are present or not. * Controls – positive (purple) – S.aureus negative ( red/pink) – E.coli 2. Endospore Stain Positive controller – B. magneterium Green spore- Positive Pink (vegetative ) – Negative 3. Acid fast Positive control – M. smeagmatis Blue – negative Pink /red- positive 4. Motility Positive control - P.vulgaris A. Non motile is negative test B. Motile is a positive test 5. Carbohydrates
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Unit 6 Lab Report 1. Objective: To find the percent composition of water in an unknown hydrate. 2. Materials: Goggles‚ apron‚ evaporating dish‚ hot plate‚ and stirring rod 3. Procedure: get the mass of a evaporating dish‚ put a sample of the hydrate onto the evaporating dish and mass the dish with hydrate. Put the dish with hydrate on the hot plate on high heat. Break up any clumps that form during the heating process. Observe any color
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MBK Lab 01 – Lab Report Name: ____________________ Section: ___________________ EXPERIMENT 1 TITLE: Observing Bacteria and Blood OBJECTIVE: To gain functional knowledge of microscope operations through practical applications of a microscope in the observation of bacteria and blood. PROCEDURES: Using the microscope‚ an oil immersion lens and observing Bacteria Cultures in Yogurt . Preparing a Blood Slide and observing Blood: After reviewing the section of the manual
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Macromolecules Lab Purpose: to determine a method of testing for macromolecules. Materials: Knowns: Unknown: Test Solutions: Glucose solution Unknown solution Iodine solution Gelatin solution Benedict’s solution Starch solution Biuret solution Oil Brown paper Water Procedure: 1. Create a data table. 2. Label 5 test tubes with known solutions. 3. Add 10-20 drops of each known solution to respective test tubes‚ do not mix pipets! 4. Add 3-5 drops of
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Microbiology 20 Laboratory Final Exam Guidelines The laboratory exam will be given on Tuesday December 4th (sections 0375 and 3252) or Thursday December 6th (section 3253) and consist of an open book portion given in CMS 105 followed by a practical portion (closed book) to be given in the lab (CMS 002). You will not need a Scantron. For the open book test‚ be sure to bring any resources you may need including your lab manual‚ lab notebook‚ safety guidelines and handouts. For the practical portion
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Grams staining method and reliability of this method. According to me‚ Grams staining process is a simple technique that assists in recognition of etiological agent and therefore can be called as one of the most significant staining practices in microbiology. Though‚ Gram is concerned that the staining process developed by him is imperfect because not all bacteria can be stained by it‚ but in my opinion‚ when we pour ethanol over the slide to wash away the dye then only definite bacteria can retain
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