University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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BioLab3 Lab Report 5 Enzymes Student Name: Cooper Lyon I. Enzyme Structure and Function EXERCISE 1 – Preparation of an enzyme activity standard At five minute intervals over the next fifteen minute period‚ record the color intensity of the solution of each test tube. Time (min) Tube S1 Potato Extract + Catechol Tube S2 Potato Extract + Water Tube S3 Catechol + Water 0 Shade of Yellow Clear/Milky Clear/Milky 5 Shade of Yellow Clear/Milky Clear/Milky 10 Orange Clear/Milky Clear/Milky 15
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with the lab equipment such as the microscope‚ square glass‚ cover slips‚ etc.; to facilitate students to observe the pond water; and enable them to discern and identify the microbes as bacteria‚ algae‚ fungi‚ or protozoa within the pond water. Additionally‚ this lab experiment will permit students to observe and distinguish the yeast suspension from the other microbes. Data/Results: (Attached) Conclusion: The student acquired the fundamental skills in which to properly handle lab equipment
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Method: as in practical guide results: Experiment 1 calibration curve- Table showing the absorbance and concentration of the 10 dilutions of p-nitrophenol at 405nm Tube no | blank | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 150 µM p-nitrophenol (ml) | 0 | 0.1 | 0.2 | 0.3 | 0.4 | 0.5 | 0.6 | 0.7 | 0.8 | 0.9 | 1.0 | Bicarbonate buffer (ml)
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Genetically Modified Organisms INTRODUCTION: The purpose of this lab was to identify if non-labeled food products are actually genetically modified foods. Before we could begin testing this theory we first had to gain an understanding about genetically modified organisms in general. This was rather easy because if you have been to any grocery store lately you have without a doubt seen products with labels saying "GMO-free" or even "contains only non-GMO ingredients." GMO actually stands for
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Instructor Biology 1111 4-5 Lab Topic 4: Microscopy Elodea Cells at ___X Elodea Cells at ___X Report Sheet—Lab Topic 4 1. Draw and label each of the organisms available. Cheek Cells at ___X Cheek Cells at ___X Name _______________________________ Date_____________ Instructor ___________________________ Section___________ _________________________ 4-6 Lab Topic 4: Microscopy 2. Fill in the following table: Compound Microscope Dissecting Microscope Types of Light Available Powers
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Submit your completed lab report to the Lab: Photosynthesis Lab assignment link for grading. For information on how this assignment will be graded‚ please visit the Course Information sectionChlorophyll and Accessory Pigments A pigment is any substance that absorbs light. The color we see comes from the wavelengths of light that reflect. Chlorophyll‚ the green pigment common to all photosynthetic cells‚ absorbs all wavelengths of visible light except green. The green reflects back to our eyes
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Goal(s): To study the effect of concentration on the rate of a reaction‚ and to find the order of the reaction in each reactant and write a rate equation. Background Theory: Chemical reactions occur at different rates that ultimately depend on their concentration‚ the temperature‚ the usage of catalysts‚ the nature of reactants‚ like the surface area of the particles. Concentration‚ for instance‚ increases the number of molecules or particles in a certain volume‚ so collisions will become more
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Potato Osmosis and Diffusion Lab Report Emily Cocq Research Question: Will there be a difference in the experimented potato cores after being subjected to high concentrations of sugar? Introduction: Water can move through protein channels in cell membrane/cell walls. The water will move due to the water potential of the cells. If there is a greater concentration of solutes (chemicals) inside the cell than outside the cell and the chemicals can not move‚ then water will respond by moving
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Natasha Tenen Lab: Thursday (3-6 pm) OSMOSIS LAB REPORT INTRODUCTION Osmosis is a special type of diffusion where water molecules move down a concentration gradient across a cell membrane. The solute (dissolved substance) concentration affects the rate of osmosis causing it either to speed the process up or slow it down. Based on this‚ how does different concentrations of sucrose affect the rate of osmosis? If sucrose concentration increases in the selectivity-permeable baggies‚ then the
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