Affinity chromatography * 8.4.1 Metal binding * 8.4.2 Immunoaffinity chromatography * 8.4.3 Purification of a tagged protein * 8.5 HPLC * 9 Concentration of the purified protein * 9.1 Lyophilization * 9.2 Ultrafiltration * 10 Analytical * 10.1 Denaturing-Condition Electrophoresis * 10.2 Non-Denaturing-Condition Electrophoresis * 11 References * 12 External links | Purpose Purification may be preparative or analytical. Preparative purifications
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of the site. In case of any leachate is detected‚ the landfill management prepared a backup plan to treat it by one of the following methods: a lagoon‚ 2 successive aeration basins for maximal COD/BOD5 and NH4-N reduction‚ an anaerobic pond‚ Ultrafiltration for retention of biomass‚ a membrane bioreactor‚ and a reverse Osmosis (RO) plant for removal of non- biodegradable COD-
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with a range of 20 to 200 μg protein‚ it can be determined by the amount of dye present in the blue ionic form. This can usually be accomplished by measuring the solution at an absorbance of 595 nm. Proteins can usually be obtained from renal ultrafiltration. Proteinuria‚ a high level of protein in urine‚ is an indicator of a varied amount of diseases that can affect the kidney. Hypertension is a well-known common cause of kidney disease and if controlled successfully‚ it can lead to a decrease in
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Purification of Infliximab[3] begins at Stage 1 with the filtration of the harvest material using a spin filter. The harvest is then clarified and undergoes 3 main chromatographic steps. Firstly‚ at Stage 2‚ the direct protein capture by Protein A affinity chromatography helps to remove a large majority of the impurities whicn include viruses and media components. At Stage 3‚ prior to cation exchange chromatography‚ the purified material in the eluted stream is then subjected to freezing‚ thawing
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This care study aims to outline the care provided to a patient during one 12hour shift. It will present the patients cause and course of ICU admission‚ identify problems both potential and actual‚ focussing particularly and two main problems. The nursing interventions preformed will be critically analysed and supported with research. Finally the care will be critically evaluated areas of possible improvement will be outlined. Patient chosen is a 36yr old male pseudonym ‘Frank’ chosen for the purpose
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some experiments using the biological activated carbon‚ it appeared that the pretreatment is useful especially since it proved to dissolve the organic compounds and hinder the growth of biological organisms [4]. Recently‚ microfiltration and ultrafiltration technologies are commonly being used in the desalination processes for its various advantages; such as‚ increasing quality of permeate and reliability in operation; however‚ the major problem occurring in such processes is as discussed previously
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Chronic Heart Failure Exacerbation Preparation Questions 1. List the risk factors for chronic left-sided heart failure related to coronary artery disease. Coronary Artery disease‚ Hypertension‚ Substance abuse‚ Dysrhythmias‚ Diabetes mellitus‚ Smoking/tobacco use‚ Obesity 2. Explain the cause of the compensations for chronic heart failure. Insufficient cardiac output causes compensatory mechanisms work to improve cardiac output. They include‚ sympathetic nervous system stimulation‚ Renin=angiotensin
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Gather information on the features of you local water supply in terms of: The Catchment Area: Sydney’s water supply is made of 5 main catchments: Warragamba‚ Woronara‚ Hawkesbury Nepean‚ Shoalhaven and Blue Mountains. Warragamba Catchment covers 9‚051km². My local area is Blacktown‚ in an image shown below; all the catchment areas are linked to Warragamba Catchment Area. Possible Sources of Contamination: Water quality in Warragamba catchment is affected by: * Wastes and runoff from
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Water Technology Hardness of Water: Natural waters containing large quantities of dissolved salts of Calcium (Ca) & Magnesium (Mg) is called hard water. It is a characteristic of preventing lather formation of water with soap. Ca2+ & Mg2+ ions react with soaps which are salts of fatty acids (stearic or palmitic acid) to give insoluble scums or precipitates of calcium or magnesium stearate or palmitate. 2C17H35COO- + Ca2+ → (C17H35COO)2 Ca (scum) 2C17H35COO- + Mg2+ → (C17H35COO)2
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1 Class XII Chemistry Ch 5: Surface Chemistry Chapter Notes Top Concepts / Key learnings: 1. 2. 3. 4. 5. 6. Adsorption: The accumulation of molecular species at the surface rather than in the bulk of a solid or liquid is termed as adsorption. Adsorbate: The substance which is being adsorbed on the surface of another substance is called adsorbate. Adsorbent: The substance present in bulk‚ on the surface of which adsorption is taking place is called adsorbent. Desorption: The process of removing an
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