Abstract:Conjugation is a natural occurring process that involves the transfer of DNA from one cell into another through a physical connection between the cells. In the following experiment‚ two strains of Escherichia coli bacterial cells (donor F’lac+strs and recipient F-lac-strr) underwent conjugation to produce a transconjugant strain (F’lac+strr). MAC plates and streptomycin were utilized to determine if conjugation had occurred. When plated‚ the donor colonies appeared red and the recipient
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MULTI-STATE INFECTION Multi- State Escherichia coli O26 Infection Linked to Raw Clover Sprouts Escherichia coli are gram- negative bacteria that are normally found within the gut of warm bloodied organisms. There are several strains of E. coli that exist as part of the normal flora of the human digestive system. They prevent harmful bacteria from establishing themselves in the intestines‚ and they also aide in the production of vitamin K2. Most E. coli strains are not harmful to humans‚ except
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The Effect of Acetyltransferases on 2 different strains of E. coli Introduction Scientists have recently discovered that resistance to antibiotics may not be such a new thing. Evidence of bacteria samples in Canadian permafrost proposes that these resistances have been around for at least 30‚000 years (Luiggi 2011). In our required pre-lab reading‚ we learned tuberculosis is becoming increasingly drug-resistant‚ giving proof that bacteria can adapt to necessary changes in order to survive (Barry
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price‚ and high degree of reduction have made glycerol a highly attractive and exploited carbon source for the production of fuels and reduced chemicals. Here we report the quantitative analysis of the fermentative metabolism of glycerol in Escherichia coli through the use of kinetic modeling and metabolic control analysis (MCA) to gain a better understanding of glycerol fermentation and identify key targets for genetic manipulation that could enhance product synthesis. The kinetics of glycerol fermentation
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E. Coli Transformation with a Plasmid DNA Containing the GFP Gene Introduction: Bacterial transformation is the process of bacteria taking in and expressing exogenous DNA. This has led to many other discoveries. In order for bacterial transformation to occur the bacteria must be in a certain physical state to be able to take in DNA. This is called competency and it allows the cell membrane to be permeable so DNA can pass through. Currently researchers are studying the transformation of E
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Commission‚ to produce legal documents related to the “isolated” outbreak‚ Although uncommon the FDA and prosecutors are working together to try to bring increased attention and punishment to companies found in outbreak violations.During the investigation E. Coli outbreaks raged through Chipotle stores across America. In total 57 consumers in twelve states were affected were hit with some strains of norovirus. Due to the investigations and outbreaks Chipotle
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Optimal Growth Conditions and Generation Time for E. coli Introduction The goals of these exercises were to determine the optimal growing condition and the generation time of E. coli. Materials and Methods Please refer to the lab manual for a complete list of materials. To determine the optimal growth condition for E. coli‚ we had to test the environmental factors that could affect the growth. We tested the relative growth of E. coli in the presence and in the absence of oxygen. The results
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The Figure 4.20 shows the profile growth of Escherichia coli type K-12 which was cultured in 20 g/L of glycerol treated with supplements from a carbon source under anaerobic condition and the medium supplemented with 10 g/L of trypton. About 10% working volume of inoculums were added to the medium for the profile growth process. The effects on growth curves of strains of Escherichia coli were investigated by measuring the concentration of succinic acid produced and its optical density by using HPLC
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Growth Dynamics of E. coli in Varying Concentrations of Nutrient Broths‚ pH‚ and in the Presence of an Antibiotic Dvora Szego‚ Elysia Preston Darcy Kmiotek‚ Brian Libby Department of Biology Rensselaer Polytechnic Institute Troy‚ NY 12180 Abstract The purpose in this experiment of growth dynamics of E. coli in varying media was to determine which media produces the maximum number of cells per unit time. First a control was established for E. coli in a 1.0x nutrient broth. This was used to compare
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The effects of black tea on the growth of E. coli bacteria cultures Kierstin Barker‚ Melissa Bischak‚ Jackie Tyszkiewicz‚ Errin Enany September 25‚ 2012 Abstract: This study was carried out in order to investigate whether black tea has antimicrobial properties as stated in Steven Johnson’s The Ghost Map. If tea does have antimicrobial properties‚ then it could aid in warding off waterborne diseases. We believed that if black tea is steeped in boiled water‚ then the amount of bacteria exposed
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