purpose of this lab is to successfully infiltrate E. coli bacterial cells with a pARA-R plasmid that is antibiotic resistant and has the rfp gene‚ or red fluorescent protein. This can be verified if the E. coli obtains the characteristics of the plasmid when it enters. To start‚ three Petri plates containing agar are needed. On each plate there is a control group and a treatment group; the treatment group being the one with the plasmid. Before the plasmid is put with the E. coli‚ first the bacteria
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Unit 7 Introduction In this piece of coursework‚ there are few amounts of ideas and experiments that I could achieved of which different products to test for my concluding idea. The type of bacteria that I am going to discuss and chosen is E-coli. I will also going to research the effectiveness of antibacterial cleaning products‚ for instance sanitizer. I will also‚ research which is the most effective product for the house hold and some other work places. Background Information What are
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Molecular Biology Lab Report Payton Jackson Introduction In this lab‚ I am going to use antibiotic-resistance plasmids to transform Escherichia coli. Materials For this lab you will need the following: LB Agar Petri dishes Beakers Test tubes CaCl2 solution Sensitive E. coli (-ampR) amp plasmids ampicillin -amp cells Water bath to heat shock cells A freezer to incubate cells Process Step 1: Wash hands and sanitize lab setting. This will prevent anything reacting with a
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Erica Osorio 5057497 Christian Roque and Rogerlio The Mechanisms by which E.Coli Cells Developed Immunities toward Ampicillin due to Plasmid and DNA Consumption U34 Abstract During the ampicillin experiment the ability to transform cells to make them adaptable to their environment was studied. The E.coli bacterial cell was used in order to observe how its DNA was able to change and develop immunity towards ampicillin. In order for this change to occur the use of several
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data on the growth of Escherichia coli (E. coli) and to monitor how it grows under certain conditions. It has been demonstrated that the levels of glucose and dissolved oxygen were found to affect the rate of growth of E. coli proportionally with a lack of oxygen resulting in the lowering of the pH. In this experiment the growth of E. coli was studied at constant temperature (37 0C) at which it grows ideally. Experimental results for the growth of Escherichia coli showed good agreement with theory
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Transformation is the genetic alteration of a cell‚ resulting from the intake of exogenous genetic material through the surrounding cell membrane. The purpose of this lab was to determine transformation of bacteria by testing the effect of P Vib plasmid of E. coli MM294‚ and how the color of the E. coli bacteria changes. In this lab‚ two small test tubes were given calcium chloride‚ E. coli MM294‚ and one of the tubes also received the plasmid P Vib. The test tubes were then placed in ice‚ heat shocked
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Jennifer Hauss March 4‚ 2015 Bacterial Transformation Lab Report Introduction In this lab‚ the goal was to transform the bacteria e-coli to glow in the dark (or under a black light). Four plates were set up with agar in them for the bacteria to feed on and grow. Changes were then made to the bacteria. One plate was the control plate‚ having only the LB or agar for the bacteria and negative pGLO‚ which is the liquid not containing the plasmid. This is the plate that was compared with the three
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E. Coli Transformation with a Plasmid DNA Containing the GFP Gene Introduction: Bacterial transformation is the process of bacteria taking in and expressing exogenous DNA. This has led to many other discoveries. In order for bacterial transformation to occur the bacteria must be in a certain physical state to be able to take in DNA. This is called competency and it allows the cell membrane to be permeable so DNA can pass through. Currently researchers are studying the transformation of E
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Abstract:Conjugation is a natural occurring process that involves the transfer of DNA from one cell into another through a physical connection between the cells. In the following experiment‚ two strains of Escherichia coli bacterial cells (donor F’lac+strs and recipient F-lac-strr) underwent conjugation to produce a transconjugant strain (F’lac+strr). MAC plates and streptomycin were utilized to determine if conjugation had occurred. When plated‚ the donor colonies appeared red and the recipient
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Material and methods Hosts ‚ plasmids and chemicals E. coli TOP10 strain was used for cloning and proliferation. E.coli BL21 DE3 was used as expression host cell. The pET 28a(+) plasmid was employed for gene expression. All chemicals were obtained from Merck Company (Germany). Codon optimization and gene synthesis Sequence encoding V-domain was obtained from Swiss-port‚ Uniprot KB and National Center for Biotechnology Information (NCBI) databases. 6x His-tag sequence was placed at N-terminal
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