"The Effect Of Substrate Concentration On Trypsin Activity" Essays and Research Papers

  • The Effect Of Substrate Concentration On Trypsin Activity

    ID:0720052 Effect of an Increasing Substrate Concentration on Enzyme Activity Rate Abstract The reaction rate of an enzyme can be affected by many factors, and the purpose of this experiment was to find out how an increasing substrate concentration influences the rate of an enzyme activity; we obtained data from recording the absorbance of the samples which contain the same amount of potato juice (enzyme oxidase) and different amount of catechol (substrate) while holding pH and temperature...

    Buffer solution, Catalysis, Chemical equilibrium 1007  Words | 4  Pages

  • Effects of Temperature, Ph, Enzyme Concentration, and Substrate Concentration on Enzymatic Activity

    Effects of Temperature, pH, Enzyme Concentration, and Substrate Concentration on Enzymatic Activity INTRODUCTION Enzymes, proteins that act as catalysts, are the most important type of protein[1]. Catalysts speed up chemical reactions and can go without being used up or changed [3] Without enzymes, the biochemical reactions that take place will react too slowly to keep up with the metabolic needs and the life functions of organisms. Catecholase is a reaction between oxygen and catechol...

    Base, Buffer solution, Catalysis 2348  Words | 7  Pages

  • The Effect of Substrate Concentration, Enzyme Concentration, Ph and Temperature on Enzyme Activity”

    Title: “The Effect of Substrate Concentration, Enzyme Concentration, pH and Temperature on Enzyme Activity” Abstract: In the following experiments we will measure precise amounts of potato extract as well as Phenylthiourea, combined with or without deionized water and in some instances change the temperature and observe and record the reaction. We will also investigate the different levels of prepared pH on varying samples of the potato extract and the Phenylthiourea and record the results....

    Acid dissociation constant, Buffer solution, Carbonic acid 1379  Words | 5  Pages

  • The Effect of Increasing Substrate Concentration on Rate of an Enzyme Reaction

    The effect of increasing substrate concentration on rate of an enzyme reaction. Enzymes are biological catalysts that lower a reactions activation energy making possible many of the reactions needed for life to exist.  Enzymes have a high specificity which have been explained by many theories such as Fischer’s lock and key. Currently the most widely accepted theory is the induced fit hypothesis proposed by Koshland in 1958. This hypothesis solves some of the problems with the Lock and key theory...

    Alpha 1-antitrypsin, Alpha 1-antitrypsin deficiency, Catalysis 1266  Words | 4  Pages

  • The Effect of Substrate Concentration on the Activity of the Enzyme Catalase

    The Effect Of Substrate Concentration On The Activity Of The Enzyme Catalase A Level Biology Project Aims This is an experiment to examine how the concentration of the substrate hydrogen peroxide affects the rate of reaction of the enzyme catalase. Background Information Enzymes such as Catalase are protein molecules which are found in living cells. They are used to speed up specific reactions in the cells. They are all very specific as each enzyme just performs one particular reaction. Catalase...

    Catalase, Chemical reaction, Enzyme 1935  Words | 6  Pages

  • The Effect of Substrate Concentration on the Enzyme Amylase

    LAB 10: NAME: DaeNia La Rodé DATE: 25TH January, 2011. FORM CLASS: L6 3 SUBJECT: Biology TITLE: Enzymes AIM: To investigate the effect of substrate concentration on the enzyme amylase INTRODUCTION: Enzymes are perhaps one of the most important proteins of the human body. Enzymes such as amylase, an enzyme that breaks down carbohydrates, work by means of surface catalysis. In other words, the surface of the enzyme enables other molecules to react in a manner they would not be able to without...

    Amylase, Catalysis, Chemical reaction 1336  Words | 4  Pages

  • Activity of a Protease

     The Activity of a Protease (Trypsin) Introduction Enzymes catalyze reactions by creating alternate reaction mechanisms whose transition states are more thermodynamically stable than uncatalyzed reactions (Berg et al., 2002; UBC Department of Microbiology and Immunology, 2006). Increased thermodynamic stability in these transition states reduces the energy of activation, the minimum amount of energy input a chemical system requires for a reaction to occur (UBC Department of Microbiology...

    Active site, Chemical equilibrium, Chemical kinetics 1974  Words | 7  Pages

  • The effect of enzyme concentration, substrate concentration, pH, and temperature on the enzyme catalase.

    The effect of enzyme concentration, substrate concentration, pH, and temperature on the enzyme catalase. Introduction: Enzymes are biological catalysts; proteins and RNA. They are required for most biological reactions and they are highly specific. Each enzyme has an active site. The active site is the spot on the enzyme where a substrate fits in. Substrates binds with enzymes through the active site. Enzymes, being highly specific, only fit with one certain substrate. Enzymes and substrates...

    Catalase, Catalysis, Chemical equilibrium 864  Words | 3  Pages

  • The Effect of Concentration, Ph and Temperature on Enzyme Activity

    The Effect of Concentration, pH and Temperature on Enzyme Activity Biology For Majors October 4, 2012 Abstract We examined the reaction an enzyme has when its concentration, pH and temperature are altered. In order to do this, we added different levels of pH into different test tubes with the enzyme (sucrose), and substrate (sucrose), and we then inverted the tube. The higher pH produced more enzyme activity. Temperature effects enzyme activity by decreasing its stability when the temperature...

    Acid dissociation constant, Buffer solution, Catalysis 2011  Words | 7  Pages

  • Effect of Substrate Concentration on the Rate of Reaction of Calase

    Name: ________________________________ Date: __________________________ Lab Partner: _____________________________________________________________________ Investigating Factors that Affect the Rate of Enzyme Activity See Textbook Pg 61. Enzymes are very large complex organic molecules that are synthesized by the cell to perform specific functions. These biological catalysts are important because they speed up the rate of the reaction that would otherwise be too...

    Catalase, Catalysis, Enzyme 820  Words | 4  Pages

  • Effect of Concentration on Enzyme Activity Lab Report

    Lab Report (Effect of concentration on enzyme activity) Biology Noor Alawadhi 11- KC Introduction: An Enzyme is a protein, which is capable of starting a chemical reaction, which involves the formation or breakage of chemical bonds. A substrate is the surface or material on or from which an organism lives, grows, or obtains its nourishment. In this case it is hydrogen peroxide. This lab report will be explaining the experiment held to understand the effects of the changes in the amount of...

    Chemical reaction, Chemistry, Concentration 619  Words | 3  Pages

  • Substrate concentration and yeast catalase

    Substrate concentration and yeast catalase Aim: To see how the substrate concentration in hydrogen peroxide affects the rate of an enzyme controlled reaction using yeast catalase. Introduction: An enzyme is a biological catalyst made of protein. Enzymes are protein molecules found in living organisms and in this case I will use a yeast catalase. Catalase is an enzyme that catalyzes the reduction of hydrogen peroxide. Hydrogen peroxide is a poisonous by-product of metabolism, so it is very...

    Catalase, Catalysis, Enzyme 1602  Words | 7  Pages

  • Investigating how pH effects the enzyme trypsin acting on coloured gelatin

     Interpretation and Evaluation of Results Interpretation: The results from the experiment determining the effects of pH on enzyme activity show that as the independent variable, pH, increases the dependent variable, percentage transmission, decreases. This is shown in the results as at the lowest pH, pH 4.0 the average percentage transmission is at its highest at 97%. At the highest pH, pH 8.0 the average transmission is 78.5%. This is also supported from the graph as it produces a negative...

    Active site, Buffer solution, Chemistry 1448  Words | 5  Pages

  • Ib Bio Experiment on Effect of Substrate Conc on Enzyme Activity

    Practical Assessment 2 – The effect of substrate concentration on the rate of enzyme activity of Catalase Aim To investigate the effect of substrate concentration (manipulated by increasing concentration of hydrogen peroxide) on the rate of enzyme activity of catalase, produced by liver cells, on the decomposition of hydrogen peroxide. Introduction Enzymes are biological catalysts that increase the rates of reactions. In an enzyme-catalyzed reaction, the substrate binds to the active site and...

    Catalase, Concentration, Enzyme 1211  Words | 5  Pages

  • Effect on Enzyme Activity

    EFFECT OF TEMPERATURE,PH AND SUBSTRATE CONCENTRATION ON ENZYME ACTIVITY | Aim To investigate the effect of Temperature,Ph and substrate concentration on the rate of enzyme activity. Hypothesis ...

    Active site, Catalase, Enzyme 1133  Words | 5  Pages

  • investigating the effect of substate concentration on the activity of catalase

    - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Effect of Substrate Concentration on Catalase Aim: An experiment to find out how the concentration of hydrogen peroxide affects the rate of reaction of the enzyme catalase. Background Information: Enzymes such as catalase are globular protein molecules with catalytic properties. A catalyst is a substrate which can alter the rate of reaction without itself undergoing any permanent change. As they are not changed...

    Catalase, Catalysis, Enzyme 978  Words | 5  Pages

  • The Effect of Substrate Concentration on the Rate of Reaction

    by the concentration of starch, or whether the results I got were due to the amounts of solution used. This would make both my results and conclusion invalid and unreliable. I also made sure that the temperature of the water bath was at the correct temperature and I kept on checking the temperature of both my amylase, and starch using a thermometer. I made sure both solutions were at the same temperature at all times throughout my experiments, so that temperature would not have an effect on my results...

    10, Green Line, Harshad number 1160  Words | 7  Pages

  • How the pH of Trypsin and Pepsin affect light transmission

    Interpretation From the graph the enzyme Trypsin has 51% light transmission at pH 2. At pH 9 the Trypsin has 39% light transmission. Between pH 2 and pH 9 the percentage of light transmission decreases at a steady rate, until it reaches pH 8 where there is a steep increase from 30% to 39% as the enzyme has reached its optimum pH at 8. During the experiment in the boiling tube this pH had the deepest red colour as the most protein gelatine was broken up. This meant...

    Acid, Amino acid, Enzyme 1087  Words | 3  Pages

  • An Investigation Into the Effects of Temperature on Enzyme Action

    An investigation into the effects of temperature on enzyme action An enzyme is a biological catalyst that speeds up the rate of reaction in certain biological functions. They play a vital role in many aspects of human physiology and are necessary for the functioning of a number of systems, for example in the digestive system to help to break down food. All enzymes have a unique active site that can fit on to a particular molecular arrangement on a target substrate; a substance e.g. carbohydrate...

    Boiling point, Celsius, Enzyme 1249  Words | 3  Pages

  • Effects of Changes in Physical Properties on Enzyme Activity

    Effects of Changes in Physical Properties on Enzyme Activity By Alex Hoffmann First Individual Lab Report Wednesday 7:30-10:15pm 10/24/12 Meghan Duell Abstract The goal of this lab was to determine the effects of certain physical properties on enzyme activity. Enzyme activity was measured by the height of the bubbles that appeared after the enzyme was added which are proportional to the rate of the reaction when time is constant. The fact that enzyme activity is affected by physical...

    Catalysis, Enzyme, Gas 1906  Words | 6  Pages

  • Effect of Temperature on Enzyme Activity

    Effect of temperature on enzyme activity In this experiment… Independent variable: Temperature of the amylase Dependent variable: Enzyme activity which is measured by the time for disappearance of starch Controlled variables: Volume of amylase; volume of starch solution; concentration of amylase; concentration of starch solution Prediction of results i) At low temperature, the rate of amylase activity is very low. ii) At optimum (=best) temperature, the rate of amylase activity is the...

    Amylase, Amylose, Energy 476  Words | 3  Pages

  • The Effect of Ph on the Activity of Catalase

    Title : THE EFFECT OF PH ON THE ACTIVITY OF CATALASE Aim : To study the effect of pH on the activity of catalase. Introduction : Catalase, an enzyme found in many different tissues, catalyses the breakdown of hydrogen peroxide into water and oxygen. 2 H202 → 2H20 + O2 Hydrogen peroxide is a toxic substance that can be formed during aerobic respiration and catalase removes this product. The activity of catalase can be measured by finding the rate of oxygen release from hydrogen peroxide...

    Acid, Acid dissociation constant, Buffer solution 1236  Words | 4  Pages

  • Effect of Temperature, Study and Measure of Enzymes Activity

    Basic Biochemistry CP2085 Effect of Temperature, Study and Measure of Enzymes Activity Abstract This experiment investigates the effect that temperature has on the rate of activity of enzyme β-galactosidase and also the rate of β-galactosidase activity in different concentration of substrate over time. Ο-nitrophenylgalactoside (ONPG) is used as a substrate for β-galactosidase. A spectrophotometer is used to detect the change in colour of the substrate. Results show that increase in temperature...

    Chemical potential, Chemical reaction, Chemistry 2441  Words | 7  Pages

  • Effect of the Nature of Different Substrates on the Rate of Cellular Respiration of Yeast

    enzyme mediated process in which organic compounds such as glucose is broken down into simpler products with the release of energy (Duka, Diaz and Villa, 2009). It is a series of metabolic processes and oxidation-reduction reactions. Oxidation of substrates, such as glucose, is a fundamental part of cellular respiration (Mader, 2009). As a catabolic process, it may or may not require the presence of oxygen. The process that requires oxygen is called aerobic respiration while the process that does not...

    Carbon dioxide, Cellular respiration, Enzyme 842  Words | 3  Pages

  • Investigating the Effect of Lipase Concentration on the Breakdown of Fat in Milk

    Investigating the Effect of Lipase Concentration on the Breakdown of Fat in Milk INTRODUCTION: Enzymes are proteins which can catalyse chemical reactions without changing themselves. The enzyme lipase breaks down the fat in dairy products such as full-cream milk for people who are lactose intolerant. Lipase acts on its specific substrate, lipids produces fatty acids. If enzyme concentration increases, random collisions between the substrates and active sites of enzyme increase due to the increasing...

    Chemistry, Concentration, Enzyme 1617  Words | 7  Pages

  • Varying Effects of Enzyme Concentration on Reaction Rates of Malate Dehydrogenase

    VARYING EFFECTS OF ENZYME CONCENTRATION ON REACTION RATES OF MALATE DEHYDROGENASE CELL BIOLOGY 13 NOVEMBER 2007 Enzymes are biological catalysts. They are proteins that speed up reactions with low concentrations. These enzyme proteins are made up of linkages of amino acids. The links coil, and coil again forming a tertiary structure. This structure has a groove in it called an active site. The active site is...

    Active site, Adenosine triphosphate, Catalysis 1156  Words | 4  Pages

  • Enzyme Substrate Complex

    Assignment 1 :- Effect of Temperature on Invertase Activity, Determining the Starting Velocity. Enzyme is the fastest known catalyst. They increase the velocity and rate of reactions in living cells. Diverse range of proteins works as enzyme with their specific temperature point, pH etc. Enzymes react with the molecules known as substrates. Binding site of an enzyme and a substrate is known as active site. The amount of enzyme present in a reaction is measured by the activity it catalyzes. The...

    Catalysis, Chemical kinetics, Chemical reaction 1501  Words | 5  Pages

  • Enzymatic Activity of Salivary Amylase

    Enzymatic Activity of Salivary Amylase Abstract: Salivary amylase is an enzyme that can digest starch molecules and break them down to sugar molecules. In this experiment, the enzymatic activity and specificity of salivary amylase was examined depending on the changes in pH and temperature. In the first part of the experiment, the effect of temperature was determined, using constant temperature bath (4, room temp, 37, 50, 60, and 70°C). Having the room temp and 50°C as the highest and 37°C as infinite...

    Buffer solution, Catalysis, Chemical reaction 1827  Words | 7  Pages

  • The Effect of Temperature on the Rate of an Enzyme Catalysed Reaction

    Aim: To find the effect of temperature on enzymes, using a potato as a catalyst. The source of catalase is in the potato cells. 2H2O2 → O2 + H2O Planning: Introduction: An Enzyme is any one of many specialised organic substances, composed of polymers of amino acids, that act as catalysts to regulate the speed of the many chemical reactions involved in the metabolism of living organisms Enzymes are classified into several broad categories, such as hydrolytic, oxidising, and reducing, depending...

    Catalase, Catalysis, Chemical reaction 1770  Words | 7  Pages

  • Effect of Temperature on Activity of Alcalase and Savinase

    Hypothesis The optimum temperatures of Alcalase and Savinase will be different. Above and below their optimum temperatures activity will decrease. Biological explanation This investigation is designed to look at the effect of temperature on the activity of the proteases Alcalase and Savinase. By the end of it I hope to know the optimum temperature of both proteases. The substrate I am going to use during the experiments is the protein gelatin, which is a translucent, colourless, brittle solid...

    Chemical equilibrium, Chemical reaction, Enzyme 1638  Words | 5  Pages

  • Enzyme Activity

    Enzyme Catalase Activity in Reaction with the Substrate Hydrogen Peroxide Abstract We performed these experiments to observe the effects of enzymes on the rate of reactions. We tested and compared the activity of the enzyme catalase on the substrate H2O2 in various states and percentages, and observed the absorption values of the enzyme-substrate relationship at different concentrations. Our results show that the more substrate available, the quicker the reaction will happen except in one test...

    Catalase, Catalysis, Chemical equilibrium 1211  Words | 5  Pages

  • An Investigationinto the Effect of Ph on the Activity of Potato Tissue Catalase

    An Investigation Into The Effect Of PH On The Activity Of Potato Tissue Catalase Aim The aim of my investigation is to see how pH affects the activity of potato tissue catalase, during the decomposition of hydrogen peroxide to produce water and oxygen. Catalase + 2H2O2 Catalase + 2H2O +O2 Catalase + Hydrogen Peroxide Catalase + Water + Oxygen Independent Variable The independent variable in this investigation is pH. Each individual enzyme has it’s own pH...

    Acid, Acid dissociation constant, Buffer solution 1537  Words | 5  Pages

  • Enzyme Activity

    Copy button on the panel above to save your report) Activity: Name: Instructor: Date: Enzyme Activity Pam Campbell Id 0002337 Dr. Murphy Nmezi August 9, 2011 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 40 °C (104 °F) 3. Sucrase activity increases with increasing sucrose concentration until a plateau is reached. Materials and Methods Effect of pH on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and...

    Acidosis, Base, Blood 1132  Words | 4  Pages

  • Factors that affect enzyme activity

    Intro I am going to investigate a factor that affects the rate of enzyme activity What are enzymes and how do they work? Enzymes are biological catalysts - catalysts are substances that increase the rate of chemical reactions without being used up. Enzymes are also proteins and thus have a specific shape. They are therefore specific in the reactions that they catalyse - one enzyme will react with molecules of one substrate. The site of the reaction occurs in an area on the surface of the protein...

    Catalase, Catalysis, Chemical reaction 1355  Words | 4  Pages

  • Effect of Temperature on Enzyme Catalase Activity in a Potato

    Effect of Temperature ( C ͦ) on Enzyme Catalase Activity in potato Aim: To investigate the Effect of temperature (10, 37, 60) Celsius (C ͦ) on enzyme catalase activity in potato using 2% of hydrogen peroxide (H202) as the substrate measuring the height (cm) of oxygen gas (bubbles) and calculating the volume of oxygen bubbles produced (cm3) Introduction: Enzymes are biological catalysts that speed up metabolic reactions without being affected. They lower the activation energy needed to start...

    Catalase, Energy, Enzyme 1315  Words | 5  Pages

  • BIO- substrate concertration

    Date: 14th-15th October Title: The effect of the concentration of hydrogen peroxide on catalase Aim: to examine how the concentration of the hydrogen peroxide affects the rate of reaction Hypothesis: The substrate concentration will determine the rate of reaction. The reaction will increase as the concentration increases, but from a certain concentration, the rate will maintain the same rate Materials: 3% hydrogen peroxide 6% hydrogen peroxide catalase round metal basin measuring cylinder ...

    Catalase, Catalysis, Concentration 733  Words | 5  Pages

  • Effect of temperature on Catalase activity

    THE EFFECT OF TEMPERATURE ON CATALASE RESEARCH QUESTION: What is the effect of various temperatures, 0°C, room temperature, 37°C, 50°C, 60°C, on the number of oxygen gas bubbles liberated, in a decomposition reaction between the enzyme Catalase, obtained from crushed mung beans, and 2% of the substrate Hydrogen Peroxide? INTRODUCTION: Enzymes are biological catalysts that increase the rate of chemical reactions without they themselves being involved in the reaction itself. Enzymes are proteins...

    Catalase, Chemical reaction, Enzyme 849  Words | 5  Pages

  • The environmental factors that effect turnip peroxidase

    changing of pH, substrate concentrations, temperature, and an inhibitor (NaCl) and the effects it hade on the enzyme turnip peroxidase. Enzymes are biological catalysts which increase reaction rates by lowering the activation energies of substrates. A substrate is a reactant that interacts with the enzyme. The enzyme and substrate can be viewed as the recently discovered "induced fit model", which suggests enzymes are flexible and dynamic things that change their shape so all these substrates (reactants)...

    Buffer solution, Catalysis, Chemical kinetics 2008  Words | 6  Pages

  • Effects of Background Music on Concentration

    Chou, P. (2007). The effects of background music on the reading performance of taiwanese esl students. New York: Self Published. Retrieved from http://books.google.com/books?id=zVz_Lv9OnN0C&pg=PA33&lpg=PA33&dq=effects of background music on concentration&source=bl&ots=xRZ4pvia4r&sig=lzJhKQmZfw7WXV-WD1hyJhT9I4A&hl=en&sa=X&ei=I3otUeKHBo_o8gSBooHYAw&ved=0CH8Q6AEwCQ * Hallman, Price, Katsarou, S. J. G. (2002, November 2).The effects of background music on...

    Background music, Effect, Learning 909  Words | 3  Pages

  • The effect that temperature has on enzyme activity.

    INVESTIGATING THE EFFECT OF TEMPERATURE ON THE RATE OF ENZYME ACTIVITY. To investigate the effect that temperature has on enzyme activity I am going to use the enzyme amylase, which is used as a biological catalyst to break down starch, which cannot pass through the gut wall due to the size of the molecules, into smaller ones. Amylase is a carbohydrase, which converts starch to simple sugars in the Salivary Glands. Three features of all enzymes are: They are always proteins. They are specific...

    Amylase, Chemical reaction, Enzyme 1615  Words | 6  Pages

  • An experiment to investigate the effect of temperature on the rate of reaction of the Enzyme Trypsin.

    An experiment to investigate the effect of temperature on the rate of reaction of the Enzyme Trypsin. Aim: This investigation was on the effect temperature has on the rate that the enzyme trypsin hydrolyses its substrate, a protein found in milk (casein). This investigation was conducted under controlled conditions, the temperature being the changeable variable. Trypsin and its substrate (powdered milk which is a source of the protein casein) were heated in a water bath. The contents of the two...

    Amino acid, Catalysis, Chemical reaction 1597  Words | 7  Pages

  • Factors That Influence Catalase Activity

    Factors That Influence Catalase Activity Sumeet Salvi 11/16/12 AP Biology Block 3 Purpose: The purpose of this experiment was to test and observe the numerous amounts of factors that influence the activity of the enzyme and that can be done by releasing oxygen gas in a fixed condition and change conditions one at a time. Hypothesis: If a protein is acquainted with a disadvantageous condition that could denature the protein, then it will not release as much Oxygen...

    Carbon dioxide, Catalase, Chemistry 1967  Words | 7  Pages

  • How does temperature affect enzyme activity?

    Enzyme Activity How does temperature affect enzyme activity? In this practical investigation, my aim is to discover how temperature will affect enzyme activity, by looking at the rate of reaction. I predict that the higher the temperature will be, the faster the reactions take place. However, I also think that there will be an optimum temperature, at which the reaction will work at its fastest; if the temperature goes beyond that, the reactions will stop altogether as the enzymes would have...

    Catalysis, Chemical equilibrium, Chemical reaction 922  Words | 3  Pages

  • Effect of Temperature on Enzyme Activity

    Experiment 4 – Effect of Temperature on Enzyme Activity Aim To study the effects of temperature on the activity of amylase enzyme on starch solution. Introduction Enzymes are widely known as biological catalyst. Almost all cellular reactions are controlled and guarded by enzymes. Virtually every metabolic reaction which takes place within a living organisms are catalyzed by enzymes. Enzymes are complex three-dimensional globular proteins. Some of the enzymes are built up off proteins and...

    Amylase, Catalysis, Energy 1023  Words | 4  Pages

  • Effects of Ph on Fungal Amylase Activity

    Effects of pH on fungal amylase activity  BI 211 November 25, 2011                   Introduction   In recent years, the uses of microorganisms have become a huge importance to industry and sparked a large interest into the exploration of enzyme activity in microorganisms.  Amylase is one of the most widely used enzyme required for the preparation of fermented foods.  Apart from food and starch industries, in which demand for them is increasing continuously, amylase is also used in...

    Amylase, Buffer solution, Digestion 1625  Words | 6  Pages

  • The Effect of Concentration on Reaction Rate

     Title of Practical: The Effect of Concentration on Reaction Rate Aim: To use a simple reaction between sodium thiosulphate and hydrochloric acid to discover concentration this determines how fast chemical reactions occur. Independent Variable: Concentration of hydrochloric acid (%) Dependent Variable: Time taken for chemical reaction to take place (sec) Hypothesis: My prediction is that the increased concentration of the thiosulfate will in turn lead to an increase in the rate of...

    Chemical reaction, Chemistry, Chlorine 874  Words | 3  Pages

  • The Effects Of Various Environmental Co

    The Effects of Various Environmental Conditions on the Rate of an Enzyme Catalyzed Reaction Olivia Payne Mr. Kuninsky 10/10/14 Variables: 1. Substrate Concentration 2. Substrate volume 3. Temperature 4. Enzyme Volume 5. Enzyme Concentration 6. pH The independent variable my group addressed was temperature The conditions for temperature were 5°C, 24°C, 35°C, and 45°C We measured the independent variable using thermometers The dependent variable was the rate of an enzyme catalyzed reaction The...

    Catalase, Catalysis, Chemical reaction 1229  Words | 9  Pages

  • Effect of Ph and Temperature on Enzyme Activity in Skeletal Muscle

    Introduction: The purpose of this experiment is to measure the effects of changes in temperatures and pH on enzyme activity in skeletal muscle, particularly the activity of lactate dehydrogenase (LDH). LDH is a glycolytic enzyme which converts pyruvate to lactate in the following equation: LDH Pyruvate+ NADH ------------ Lactate + NAD The reaction above can move in both directions,...

    Adenosine triphosphate, Catalysis, Cellular respiration 1735  Words | 5  Pages

  • How Enzyme Concentration Can Affect Rate of Reaction

    How Enzyme Concentration can Affect Rate of Reaction The purpose of this investigation was to see how the concentration of an enzyme affected the rate at which a substance was broken down. We did this by using a white protein called casein. Casein is found in milk powder, it is a protein and used mainly as a binding agent in foods, because it is mad to proteins and joins to a phosphoric acid it belong to a group called the phophoproteins. In terms of in milk it is said to be healthier if it is...

    Acid, Amino acid, Catalysis 857  Words | 4  Pages

  • Effects of Enzymes

    The Effects on Enzymes By Bailey Rose The Effects on Enzymes Bailey Rose 10/31/2011 Abstract In this lab exercise, the study of enzyme catalase, we viewed the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase from starch and measure the rate of activity under different conditions.  Changes in temperature and pH along with Substrate Concentration and Enzyme Concentration were the conditions tested in the experiment.  Our class performed...

    Chemical reaction, Chemistry, Enzyme 1589  Words | 5  Pages

  • The Effect of substrate on the Rate of Respiration on Yeast

     The Effect of substrate on the Rate of Respiration on Yeast1 Justine Maturan Group 4 Sec. Y – 5L November 18, 2014 ________________________________________________________________ 1A scientific paper submitted in partial fulfilment of the requirements in General Biology I Laboratory under Prof. Susan Sedano, 1st semester 2014-2015 ABSTRACT In order to determine the effect of the substrate on the rate of respiration of yeast, Durham test tube method was used in the first experiment...

    Carbon dioxide, Cellular respiration, Enzyme 2137  Words | 8  Pages

  • Effects of pH on Amylase Activity

    The Determination of the Effect of pH on Amylase Activity Grace Chung Abstract: Amylase is an important enzyme in the human body as it allows for the consumption of starch by breaking the polysaccharide down into maltose units. All enzymes, including amylase, function best at a certain optimal pH. Therefore, in this experiment, the effect of different pHs on the reaction rate of amylase is studied. It was hypothesized that the amylase-starch reaction would proceed fastest at a pH closest to...

    Amylase, Buffer solution, Chemical reaction 2067  Words | 8  Pages

  • Substrate Specificity of Invertase

    as the active site. The nature and arrangement of amino acids in the active site make it specific for only one type of substrate. Enzymes catalyze an unfathomable number of reactions by using a combination of only six basic mechanisms: (1) acid-base catalysis; (2) covalent catalysis; (3) metal ion catalysis; (4) electrostatic catalysis; (5) proximity and orientation effects; and (6) preferential binding of the transition state complex. Independent of the mechanistic characteristics taken to...

    Catalysis, Disaccharide, Enzyme 1796  Words | 7  Pages

  • Factors affecting the rate of Enzyme activity

    Affecting the Rate of Enzyme Activity The Purpose of doing this experiment is “how factors affect on the rate of enzyme activity.” “We examine the affects of enzyme activity when exposing enzymes to different substrates, PH and temperature. In this experiment a type of enzyme called Peroxides has been used. Peroxidase is the enzymes are found in living organism so, it can be founded in both liver and potato. For measuring the rate of enzyme activity in each experiment the height...

    Catalase, Enzyme, Glucose 1642  Words | 5  Pages

  • Enzyme Lab Report

     The Effect of Substrate Concentration on Initial Reaction Rate The purpose of this study is to identify the relationship between substrate concentration and initial reaction rate provided that substrate concentration is much greater than enzyme concentration. Enzymes are essential to life as they are required for many vital metabolic reactions to occur. To adequately explain the properties of enzymes, it is assumed that an enzyme-controlled reaction takes place through an enzyme-substrate...

    Chemical equilibrium, Chemical reaction, Chemistry 1656  Words | 5  Pages

  • Effect of pH on Invertase Activity

    EFFECT OF pH LEVEL ON THE ENZYME ACTIVITY OF INVERTASE John Dale Cruz, Michael Ray Dela Cruz, Karen Angelica Domalaon, Abegail Dulay, Shirley Ann Felipe Group 5 2C Medical Technology Biochemistry Laboratory ABSTRACT Invertase is a type of enzyme, a natural catalytic agent for biochemical reactions, can be obtained in Baker’s Yeast. Determination of the effect of pH on invertase activity is the primary objective of the experiment. Dinitrosalicyclic acid (DNS) Assay method is utilized to monitor...

    Acid dissociation constant, Buffer solution, Buffering agent 1172  Words | 7  Pages

  • Effect of Concentration on Enzyme Activity Lab Report

    Explain the technique by which copper can be purified. What is a use of pure copper? 19) Explain the processes of phytomining and bioleaching. 20) Why is recycling beneficial to the environment? 21) What % of iron comes from the blast furnace? What effect does this have on its properties? 22) Give some properties of low-carbon, high-carbon and stainless steels. 23) Why might an alloy of aluminium be preferable to the pure metal in aeroplane manufacture? 24) What name is given to the metals in the...

    Atom, Carbon, Carbon dioxide 799  Words | 3  Pages

  • The Effect of the Nature of Substrate on Cellular respiration of yeast

    Abstract The experiment aims to observe if simpler substrates makes the rate of cellular respiration faster. Using yeast, smith fermentation tubes and different substrates namely, starch, lactose, sucrose, glucose and fructose, which are from different kinds of carbohydrates, ranging from the simplest sugars glucose and fructose to the polysaccharide starch and water as the control, the hypothesis was tested. With the span of thirty minutes with five-minute intervals, the height of carbon dioxide...

    Carbon dioxide, Cellular respiration, Disaccharide 1775  Words | 6  Pages

  • Enzyme Activity Over Different Concentrations and Effects

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