• Activity of a Protease
    activity is of great interest and is further investigated here. The effects of enzyme and substrate concentrations, as well as the effects of pH, on the initial rates of BAPNA hydrolysis by trypsin are investigated. Additionally, the inhibitory activity of the Jack Beans trypsin inhibitor is determined...
    Premium 1974 Words 8 Pages
  • Ccea as Biology Coursework: an Investigation to Measure the Percentage Light Transmission Through a Solution in Which Jelly Cubes Were Immersed over a 24 Hour Period
    acidic solution than the specific optimum pH. When an enzyme structure is altered it cannot fit successfully with the substrate. Activity is therefore limited to a few enzyme molecules that are still unaltered or may totally stop. The protein digesting enzymes, pepsin and trypsin, will hydrolyse the...
    Premium 1454 Words 6 Pages
  • How the pH of Trypsin and Pepsin affect light transmission
    attachment and the enzyme is denatured. The volume of the enzyme solution was kept the same as every enzyme works best at different concentrations; if there is too much enzyme concentration then the enzyme activity will level off as there is not any more substrate active sites that need to be filled. We...
    Premium 1087 Words 5 Pages
  • Behavior of Proteins
    concentration of the reactants are so high that the enzyme is completely saturated with reactant molecules. Enzyme-Substrate Binding Substrate- binds (noncovalent interaction) in the active site of the enzyme to form a complex. F of Complex-> F of transition-state-> F of product Why do...
    Premium 3630 Words 15 Pages
  • Investigating how pH effects the enzyme trypsin acting on coloured gelatin
     Interpretation and Evaluation of Results Interpretation: The results from the experiment determining the effects of pH on enzyme activity show that as the independent variable, pH, increases the dependent variable, percentage transmission, decreases. This is shown in the results as at the...
    Premium 1448 Words 6 Pages
  • An Investigation Into the Effects of Temperature on Enzyme Action
    effectively over a range of discreet temperature settings. These included measurements from 20 degrees c, to 60 degrees c, in ten degree intervals and included a measurement at 0 degrees c, for comparison. The substrate used was protein from skimmed milk. Method A one per-cent solution of trypsin in...
    Premium 1249 Words 5 Pages
  • Enzyme Regulation
    substrate binding to one catalytic subunit reduces the affinity of the other catalytic sites for substrate (Strater et al). The cooperativity effect enables cells to produce enzymes that are more sensitive or less sensitive to changes in substrate concentration than would otherwise be predicted by...
    Premium 1727 Words 7 Pages
  • Biology
    [Turn over November 2008 Paper 22 3 Trypsin is a protease enzyme, which hydrolyses protein molecules, such as albumen, to amino acids. A student investigated the effect of substrate concentration on the activity of trypsin. Six different concentrations of albumen were prepared and...
    Premium 3153 Words 13 Pages
  • Recognise the Characteristics of Enzymes and Their Involvement in Physiological Processes
    ) (www.bing.com) Substrate concentration has an effect As the concentration of substrate increases, the rate of reaction also increases until the point saturation occurs. It means as you increase the concentration, rate keeps increasing and then one point comes when the maximum rate is achieved and there is...
    Premium 1258 Words 6 Pages
  • All About Enzymes
    enzyme activity include; Temperature, pH concentration, enzyme concentration and substrate concentration. What Are They Used For? Enzymes are not only for the body, they are also used in labs, factories and kitchens to create industrial or personal products, for example bread is made within a...
    Premium 2441 Words 10 Pages
  • Enzyems
    The Effect Of Enzyme Concentration On Enzyme Activity The pancreatic duct in individuals who have cystic fibrosis frequently becomes blocked, reducing or preventing the release of pancreatic enzymes into the small intestine. The aim of this activity is to investigate the effect of a...
    Premium 1800 Words 8 Pages
  • Bio Questions
    Which graph shows the effects of substrate concentration on an enzyme- catalysed reaction at a fixed enzyme concentration? [pic] A student soaked a duck egg in a saturated salt solution. After a few days, he found that the egg tasted salty. Which of the following...
    Premium 1555 Words 7 Pages
  • Enzyme Mechanism
    transition state, stabilizing it, and is potently inhibited by transition state analogs. Serine Protease Mechanism: Multiple Catalytic Mechanisms at Work Structure of the Serine Protease Chymotrypsin Serine Protease Substrate Specificity and Active-Site Pockets Trypsin cleaves amide bond...
    Premium 834 Words 4 Pages
  • Corn Gluten Hydrolysis by Alcalase: Effects of Process Parameters on Hydrolysis, Solubilization and Enzyme Inactivation
    enzyme activity was performed to investigate the effect of the process conditions such as substrate concentration, enzyme concentration, temperature and pH. The kinetics of hydrolysis, solubilization and enzyme inactivation were examined for all experimental conditions applied. Materials and methods...
    Premium 4473 Words 18 Pages
  • Biochemistry
    ATP. cAMP subsequently activates a key enzyme: protein kinase A (PKA). The kinase alters the activities of target proteins by phosphorylating specific serine or threonine residues. Most effects of cAMP in eukaryotic cells are achieved through the activation by cAMP of PKA. The binding of cAMP to...
    Premium 8306 Words 34 Pages
  • Biochem Study Guide for Test 2
    rapidly toward Vmax. * Cooperativity means that allosteric enzymes display a threshold effect: below a certain substrate concentration, there is little enzyme activity. However, after the threshold has been reached, enzyme activity increases rapidly. In other words, much like an “on or off” switch...
    Premium 17491 Words 70 Pages
  • Biology and Physic Experiment
    enzymes are proteins, they can be denatured in a variety of ways, so they are most active under mild conditions. Most enzymes have optimum activity at a neutral pH and at body temperature. Enzymes are also very specific – they only act on one substrate or one class of related substrate molecules...
    Premium 985 Words 4 Pages
  • Enzymes
    oxidation. As a consequence, any given enzyme is dependent on the activity of preceding reaction steps for its substrate. 3. Zymogens / pro-enzymes Proteolytic enzymes (protease) such as trypsin & chymotrypsin are synthesized in their precursor form and are inactive (zymogen/pro-enzyme). This is...
    Premium 3036 Words 13 Pages
  • research
    byreductive methylation. When the pH is adjusted back to pH 8, activity returns. The Effect of Temperature on the Protease Enzyme Trypsin http://www.123helpme.com/view.asp?id=122018 Trypsin is an enzyme, enzymes exist in all living things. They are  composed of polymers of amino...
    Premium 1683 Words 7 Pages
  • catalase experiement
    . Figure 2. Effect of enzyme concentration on the rate of reaction 2. Substrate Concentration For any given enzyme, there is some maximum number of substrate molecules that it can transform or turn over each minute. If this number is very large, the reaction will have a high maximum rate. Now...
    Premium 3943 Words 16 Pages