Sterile filter paper disc PROCEDURE i. The bottom of a petri dish has been labeled with the name of the organism‚ the chemical agent and name and date of experimenter and experiment respectively. ii. One or two loopfuls of the organism has been taken from the cultures provided and a tube of liquid nutrient agar has been aseptically inoculated at 50oC. The mixture has been poured into a sterile petri dish. iii. When the
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petri dishes at a tilted angle‚ with the petri dish lids at an angle as well to reduce airborne contamination. After the end of each pour—no more than 2/3 of the petri dish’s capacity—the flask containing the liquid media was sterilized briefly through the Bunsen-burner by running it through the flames a couple of times for a few seconds‚ then the aluminum foil was also briefly sterilized by this same method. These lids were closed on the petri dish and left
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second agar plate‚ open the “stick” end of the sterile cotton swabs to avoid contamination of the swab. Deep the swab in to the sterile water and collect a dust form the corner of the table by swab and rub the swab over the entire surface of the Petri dish without going back over areas you have already swabbed. 3. For the third agar plate‚ divide the plate in two different parts like washed and unwashed finger tip and swipe on each side of plate‚ see the difference between them. 4. For the forth
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Aim: To investigate the effect to the potato cells in the different solute concentration water Introduction; Water can move through the different cells due to the difference of water potentials in the cells. If there is a higher solute concentration in the cell than outside the cell‚ the water will move into the cell. However‚ if the concentration of inside the cell is lower than the outside‚ water will not move into the cell. This process is called osmosis. Research question; This investigation
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Materials: The materials we used in this lab were a petri dish with agar‚ forceps‚ water‚ alcohol‚ hydrogen peroxide‚ antibacterial soap‚ filter paper disc‚ tape‚ and a permanent marker. Procedure: We started this lab on May 12‚ 2014. Using a permanent marker on the bottom of the dish‚ we divided it into 4 sections. We labeled the sections‚ W (water)‚ A (alcohol)‚ S (soap)‚ and HP (hydrogen peroxide). Then‚ we labeled the perimeter of our dish with our initials and class period. Kyra rubbed her fingers
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INTODUCTORY PLANT PATHOLOGY C123P1 Isolation & Study of Sclerotinia (Monilinia) Fructigena Extraction of Polygalacturonase(PG) enzyme Assay Sclerotinia (Monilinia) Fructigena • 10g of infected tissue was taken and ground in a mortar and pestle with 100Mm PH5 citrate buffer then filtered through 4 layers of muslin. • Half of this mixture was boiled at 50°C for 10 minutes and the other half was left at room temperature. • The above steps were repeated with a sample with healthy
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How to Carry Out Aseptic Techniques in a Batch Culture and in the Laboratory | | | | | | | | | |The batch vessel should be sterilised beforehand using steam. The nutrient medium that is added to the vessel |
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Introduction- The purpose of this experiment is to obtain isolation of individual species of particles from the mixed culture. This is completed through the isolation technique of streak plate. The objective of this experiment is to replicate the technique of streak plate but on a much larger scale. Because it is on a larger scale the particles are able to be visually observed as they are isolated using the streaking technique as the experiment is conducted. The benefits of the streaking technique
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Planarian Lab Report Hanna Klein Bishop Fenwick High School Planarian Lab Report Introduction Planarian worms are free-living flatworms that are not parasitic. Planarians have a three-cavity digestive system. Planarians are usually either carnivorous or scavengers. Planarians may eat other living‚ as well as dead‚ invertebrates‚ detritus or decaying organic matter. Planarians do not realize that are eating a lot‚ and can over eat resulting in death. Most planarians are freshwater forms that can
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How would you compare the life of the poor Egyptian boy in ‘The Conjurer Made off with the Dish’ with that of a poor boy living in a metropolitan city like Karachi? “The day was passing and soon mysterious darkness would descend”. This is how Naguib Mahfouz’s story ends. A short story filled with a lifetime of experiences that perhaps every poor boy in an urban city faces. Experiences of violence‚ sex‚ power struggle‚ poverty‚ failure‚ love‚ hard choices are what make this story of a young
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