Bunsen-Burner Reaction Lab Report

First, the work area was sanitized with ethanol and paper towels, and the Bunsen-burner was lighted. The inoculating loops were sterilized by setting them above the cone of the flame to make the loop red, then left them to cool in the test tube tack for streaking. The media included a liquid (YED media that was made by 3g yeast extract, 6g anhydrous dextrose, 6g agar, and 300 mL tap water). The liquid media was on a hot plate, then an oven mitt was used to carefully transfer onto 4 petri dishes at a tilted angle, with the petri dish lids at an angle as well to reduce airborne contamination. After the end of each pour—no more than 2/3 of the petri dish’s capacity—the flask containing the liquid media was sterilized briefly through the Bunsen-burner by running it through the flames a couple of times for a few seconds, then the aluminum foil was also briefly sterilized by this same method. These lids were closed on the petri dish and left
Using a micropipette with sterilized 0.1 mL yellow tips from its box, 0.1 mL of test tube C was transferred onto the turning table with the lid over the petri dish to reduce airborne contamination, and the petri dish was spun with the metal spreader over it to spread its contents onto the petri dish. The yellow tip was then discarded into its appropriate container. The metal spreader was then sterilized via the method mentioned earlier. The petri dish was left upside down, again to prevent condensation and reducing its potential disruption to its growth pattern. These steps were repeated for test tube D and E. The metal spreader was then sterilized again for any future uses. These plates for C, D, E, and the streaking plate were left upside down, taped and labeled with the section and lab ID number and then incubated for 2 days at 30 degrees Celsius and refrigerated until the following lab