Macromolecules: Glucose and Lab Manual Pg.

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Exercise 4.4: Identification of Macromolecules in Household Substances

The purpose of this lab is to determine the presence of glucose, starch, and protein in various samples through standard tests.

Solution 1: Formula

I believe formula will test positive for sugar and protein since it is supposed to be a nutritious, balanced drink for infants.

Materials and Methods: Please refer to the Biology 1406 Lab Manual Pg. 43 Test For| Coloration/
Appearance of Positive Control| Coloration/ Appearance of Negative Control| Sol. 1 Result
(+ or -)| Comments|
Reducing Sugars
(tube 1)|
Opaque Orange|
Stays Blue| +| Strong in sugar content|
Starch
(tube 2)|
Bluish Black|
Orange-Yellow| -| None|
Proteins(tube 3)|
Purple|
Light Blue| +| None|

Results:

Conclusion: I tested the solution with Benedict’s, Iodine, and Biuret’s to determine what it contains. Formula tested positive for sugar and protein which proved my hypothesis to be correct.

Solution #2: Chicken Broth

Chicken broth will test positive for protein and starch because chickens have a high content of protein in their eggs and meat.

Materials and Methods: Please refer to the Biology 1406 Lab Manual Pg. 43 and 44

Results:

Test For| Coloration/
Appearance of Positive Control| Coloration/
Appearance of Negative Control| Sol. 2
Result
(+ or -)|
Comments|
Reducing Sugars
(tube 4)| Opaque Orange| Stays Blue| +| Slow change in color from green to orange| Starch
(tube 5)| Bluish Black| Orange-Yellow| +| Quick change| Proteins
(tube 6)| Purple| Light Blue| +| Quick Change|

Conclusion: I tested the solution with Benedict’s, Iodine, and Biuret’s to determine what it contains. Chicken broth tested positive for reducing sugars, starch and proteins. Part of my hypothesis was proved wrong because the solution did contain sugar.

Solution 3: Lemon-Lime Soda

Lemon-Lime Soda will test positive for reducing sugars...
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