In this lab we have focus on Isolation of bacteria from environment. Microorganisms are found throughout the environment: in the air and water; on the surface of any object such as clothes, walls, furniture; in soil and dust; and on and in our own bodies (skin and mucous membranes). In order to demonstrate the ubiquity and diversity of microbes in the environment, samples from immediate areas of the environment and/or from your body will be obtained and cultured.
4 sterile cotton swab, sterile water in test tube, 4 agar plates and 2 blood agar plates, wax pencil and labels.
Appropriately label the cover of each plate as indicated in lab.
1.Determine 2 sampling sources, one from your body and one from the surrounding environment. 2.For the first agar plate, for sampling from air, remove the lids from the plate and allow it to sit uncovered for 15 minutes. 1.For second agar plate, open the “stick” end of the sterile cotton swabs to avoid contamination of the swab. Deep the swab in to the sterile water and collect a dust form the corner of the table by swab and rub the swab over the entire surface of the Petri dish without going back over areas you have already swabbed. 3.For the third agar plate, divide the plate in two different parts like washed and unwashed finger tip and swipe on each side of plate, see the difference between them. 4.For the forth agar plate, divide it into the four equal part and tested four different place for bacterial grow. In first part, remove the sterile cotton swab from the package and immerse it in the sterile water and take the bacteria from forearm and swipe the swab on first part. For second part, again take the sterile cotton swab and immerse it in sterile water, and take the bacteria from back of ear and rub on the second part. For third part, again take the sterile cotton swab and immerse it in sterile water, and take the bacteria from handle of...