OUTLINE What is PCR and Gel Electrophoresis? • Polymerase chain reaction (PCR) is a technique which is used to amplify the number of copies of a specific region of DNA‚ in order to produce enough DNA to be adequately tested. This technique can be used to identify with a very high-probability‚ disease-causing viruses and/or bacteria‚ a deceased person‚ or a criminal suspect. • Gel electrophoresis is a widely used technique for separating electrically charged molecules. It is a central technique
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Abstract There are many methods employed to precipitate proteins out of solution. In this experiment we manipulated many physical and chemical variables in order to achieve purification of a protein via precipitation. In the first part of the experiment we purified the protein casein by modifying it’s pH. In the second part of the experiment we manipulated the ionic strength of albumin in egg whites‚ in a process called salting out. By manipulating these chemical properties we were able to
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What is gel electrophoresis? Gel electrophoresis is a technique that separates pieces of DNA (or other biological molecules) by size. Pieces of DNA in a test tube all look the same. DNA Gel Electrophoresis Gel electrophoresis separates pieces of DNA by size so that researchers can further analyze them BURST Training Session November 29‚ 2005 Once the DNA samples are loaded onto the gel‚ an electric current is applied to the gel. DNA is negatively charged due to all the phosphate groups in the
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Laura Gallagher Partner: Rob Einersen Biology Period D Mr. Alvarez 15 February 2013 Gel Electrophoresis Introduction: Agarose Gel Electrophoresis is a process in which the process of determining whether a strand of DNA is either positively or negatively charged. The container in which the gel is stored has a negative and positive side; whichever side the DNA molecules go to means the DNA is charged the opposite way. (Ware‚ Lunte‚ Gardiner)For example if a DNA molecule
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Report on Receptor Proteins According to Abedon (2012)‚ receptor proteins are intracellular proteins‚ or protein fractions‚ that have a high specific affinity for binding a known stimulus to cellular activity. Receptor proteins may be located at cell surfaces or within the interior of cells. They may detect hormones or instead keep track of what otherwise is going on in cell ’s environment. They may be enzymes or serve to relay the occurrence of signal reception in other ways such as by opening
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examine the physical structure of DNA‚ its nucleotide sequence and how genes are read and regulated. One key tool is the ability to visualize DNA molecules and determine their length by using a technique called gel electrophoresis. Introduction to gel electrophoresis In gel electrophoresis‚ DNA fragments move through a porous matrix made of agarose‚ a gelatin-like substance purified from seaweed. The agarose is melted like Jell-O and then ® poured into a plastic tray to harden into a slab called
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Lab Report An enzyme is a protein that speeds up the rates of chemical reactions. They recognize‚ bind‚ and change specific reactants. They do not change so they can catalyze the same reaction again and again. Activation energy is the amount of energy needed in order to begin a chemical reaction. A Catalyst is a substance that increases the rate of a chemical reaction without itself undergoing any permanent chemical change. Catalysts are substances or a substance that configures another substance
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Student’s Name Date of Experiment- 11/27/12 Date Report Submitted 11/27/12 Title: Caloric Content of Food Purpose: to be able to measure the energy content of foods Procedure: We are going to take food items and burn them to heat water to be able to determine the amount of “energy” a food source can emit. Data Tables: |Data Table 1: Food Item - Observations
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Lab #4: The Immune System Purpose: The purpose of this lab was to perform and understand the procedures of conducting an ELISA test to determine whether a particular antibody is present in a patient’s blood sample through a virtual simulation. Hypothesis: If I successfully complete this lab‚ I will then understand how to perform an ELISA test‚ the purpose an ELISA test‚ and also how to interpret the results of this test. Materials and Procedures: Materials: Howard Huges medical
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cheese. Lactic acid bacteria(LAB)‚ a bacteria that can be found in the production of cheese‚ its stress gene was investigated in the experiment by using various biochemical and genetic techniques to identify and extract. The characterisation of the strain illustrates how identification of strains differ using different methods‚ such as gram stain and 16s rRNA screening. After the characterisation‚ the stress gene isolation assist the further understanding of the gene on LAB be giving different stress
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