course of five years (2002- 2007) to test many foods‚ such as tomatoes and rice‚ for approved and unapproved Genetically Modified Organisms. Similar to the experiment conducted in the botany lab‚ the scientists involved in this study used a Polymerase Chain Reaction‚ or PCR‚ method to determine their results (Kyrova‚ Ostry‚ Laichmannova‚ Ruprich‚ 2010). Enrico Dainese and his partners did another similar study‚ on soybeans specifically. Like our experiment conducted on the cornbread mix‚ Dainese and
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RAPD Profile‚ Antioxidant and Antimicrobial Potential in Zingiber officinale Rosc. collected from different ecological zones of India Sapna Sharma1‚ Jyotsna Singh1 Archana Kumari1‚ Chetna Mishra1‚ Poonam Kakkar1* Herbal Research Section‚ Indian Institute of Toxicology Research‚ P.O. Box No. 80‚ Lucknow‚ India *Correspondence to: Dr (Mrs) P. Kakkar Head‚ Herbal Research Industrial Toxicology Research Centre Post Box No.80‚ M.G. Marg‚ Lucknow-226 001‚ India Tel: (+91)-0522-2213786
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Analysis of the RBM22 Protein Association with Gene Expression through Affinity Tagging Lucas Akin Stream Faculty: Scott Stevens Research Professor: Albert MacKrell University of Texas at Austin School of Biological Sciences Abstract Affinity tagging of proteins is an important method for biochemical analyses [4] and has proven effective in further expanding our knowledge of their specific functions. A group of interactive proteins expressed by bacteriophage ʎ known as exo‚ bet‚ and
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34. Mixture for overlapping PCR: 1 μL of Herculase II Fusion DNA polymerase‚ 10 μL of 5X Herculase II reaction buffer‚ 0.5 μL of a 100mM solution of dNTPs (25 mM each)‚ 2 μL of a 10 μM solution of each primer‚ 100 ng of each upstream and downstream fragments‚ 200 ng of pyrG marker fragment and adjust to 50 μL of double-distilled water. 35. Lysis Buffer: to prepare 50 mL of buffer dissolve 23.6 g of Guanidine thiocyanate (118.16 g/L) in 25 mL of double-distilled water. Once dissolved add: 2.5 mL of
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3.2.4 Genomic DNA extraction of the root tips After the treatment of the onion bulbs with lake water sample at different period of time‚ the DNA from the root tips will be ready to be extracted and observed. First of all‚ the genomic of the Allium cepa will be easily done by the already available Uneasy® Plant Mini kit (Qiagen‚ Germany) in the laboratory. All of the procedures can be performed by referring to the manual provided by the kit. A very small amount of root tips that weigh 100 mg will
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8.3 POLYMORPHISMS DETECTED BY PCR Without a doubt‚ the polymerase chain reaction (PCR) represents the single most important technique in the field of molecular biology today. What PCR accomplishes in technical terms can be described very simply — it allows the rapid and unlimited amplification of specific nucleic acid sequences that may be present at very low concentrations in very complex mixtures. Within less than a decade after its initial development‚ it has become a critical tool for all practicing
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A molecular method for identifying Calicophoron infecting cattle in South Africa Lavinia Perumal School of Biological Sciences and Conservation‚ University of KwaZulu-Natal‚ Westville‚ Durban. Email:209512772@ukzn.ac.za Abstract Calicophoron species were collected from the ruminants of cattle from a Kokstad abattoir in South Africa. The second internal transcribed spacer (ITS-2) of the ribosomal DNA had been used as the genetic marker in this molecular study. In an attempt to identify as
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ARTICLE Received 6 Sep 2013 | Accepted 5 Nov 2013 | Published 2 Dec 2013 DOI: 10.1038/ncomms3873 OPEN Mutational landscape of gingivo-buccal oral squamous cell carcinoma reveals new recurrently-mutated genes and molecular subgroups India Project Team of the International Cancer Genome Consortium1 Gingivo-buccal oral squamous cell carcinoma (OSCC-GB)‚ an anatomical and clinical subtype of head and neck squamous cell carcinoma (HNSCC)‚ is prevalent in regions where tobaccochewing
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characteristics of nucleotides __ A comparison of RNA and DNA (other than uracil substitution) __ The triplet arrangement of codons and/or anticodons __ The control of transcription (Operon‚ etc.) __ Promoters __ The role of polymerase __ Intervening sequences in eukaryotic cells __ Factors involved in the release of mRNA from DNA __ 5’ - 3’ arrangement with attachment at -OH end A definition of translation was worth an additional point with one point given for
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Systematic and Applied Microbiology 34 (2011) 127–138 Contents lists available at ScienceDirect Systematic and Applied Microbiology journal homepage: www.elsevier.de/syapm Microbial ecology of autothermal thermophilic aerobic digester (ATAD) systems for treating waste activated sludge David Hayes‚ Leonard Izzard 1 ‚ Robert Seviour ∗ Biotechnology Research Centre‚ La Trobe University‚ Bendigo‚ VIC 3552‚ Australia a r t i c l e i n f o Article history: Received 3 June 2010 Keywords:
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