bacterial species (15‚ 16). The clonal variability in different bacterial species such as E.coli is achieved throughout the application of primers which are homologous to ERIC sequences. The gained patterns which are appeared in the following of PCR reactions are valuable for evaluating the level of relationships (17). For this reason‚ the major purpose of present survey is to determine the distribution of ERICs within the isolated strains of E.coli as an appropriate and quick molecular-genomic tool.
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DNA electrophoresis.BioTechniques36:214-216 Chue J‚Smith CA(2011)Sex determination and sexual differentiation in the avian model.FEBS J278‚ 1027–1034. Ito H‚ Sudo-Yamaji A‚Abe M‚Murase T‚Tsubota T (2003)Sex identification by alternative polymerase chain reaction methods in Falconiformes.Zoological Science20: 339–344. McDonald P‚ GRIFFITH S (2011)To pluck or not to pluck: the hidden ethical and scientific costs of relying on feathers as a primary source of DNA.Journal of Avian Biology 42 (3): 197-
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individuals have the same DNA. This makes it easy to determine a match between the person the blood came from and the blood sample. Alec Jeffrey’s came up with the first genetic profile in 1984. Jeffery’s invented the PCR‚ also known as the polymerase chain reaction. 2. How are computers used in fingerprint analysis? - Computers hold hundreds of fingerprint samples that have been recorded through time. When a fingerprint is found‚ it can be run through the system to try and find an identical fingerprint
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radioactive atom or a fluorescent dye is used as label on probe. Reverse Transcription Polymerase Chain Reaction (RT)-PCR Employing PCR-based procedure in analyzing RNA would require cDNA to be synthesized using reverse transcription. When reverse transcription and polymerase chain reaction are combined together‚ it will permit detection of low abundance RNAs. RT-PCR is distinct from Real Time Polymerase Chain Reaction (qPCR). The latter measures the amplification of DNA in quantitative way and it uses
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Genetically Modified Organisms INTRODUCTION: The purpose of this lab was to identify if non-labeled food products are actually genetically modified foods. Before we could begin testing this theory we first had to gain an understanding about genetically modified organisms in general. This was rather easy because if you have been to any grocery store lately you have without a doubt seen products with labels saying "GMO-free" or even "contains only non-GMO ingredients." GMO actually stands for
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Assignment 2: Answer Sheet (LSM 1401 Semester 2 AY2011/12) Nucleic Acid Virtual Laboratories |Name | | |Matriculation number | | |A. DNA Extraction Virtual Lab [2 marks]
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frame of ten weeks conducted by a student team of four. The proposed methodology to take place is to cultivate and purify isolates from the soil‚ find antibiotic isolates‚ isolate the genomic DNA‚ to amplify the 16S rRNA gene segments by polymerase chain reaction‚ and to build a phylogenetic tree. Lantana is a genus of about 150 species of flowering plants popularly used as antirheumatic‚ stimulant‚ antibacterial‚ biologic control and as ornamental plant (Dua VK et al.‚ 1996). Lantana camara is
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amplification of the DNA templates was performed and the products obtained were used to perform gel electrophoresis. The process of PCR allows for the amplification of the DNA samples and the components needed to perform PCR are template DNA‚ DNA polymerase‚ primers‚ buffer‚ magnesium and nucleotides (dNTPs or deoxy nucleotide triphosphates). [7] DNA samples were added to a master mix‚ which provides the key ingredients‚ which are necessary for performing PCR. These components are premixed and optimized
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Methods to elucidate gene function Historically‚ studies to identify genes that function in a particular process involve forward genetics. One way to mutate genes using this process is to expose organisms to a mutagen (typically either a chemical or gamma radiation)‚ randomly mutating the genome in many animals. We then screen these animals for defects in the process we wish to study – essentially‚ looking for physical or behavioral changes in the organism. Mutagenesis (creating mutations) is
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Application of Molecular Techniques for Detection of Disease Resistant Genes in Tomato Breeding Lines for Guatemala Objectives: 1. Evaluate and modify methods for detection of Fusarium Race 2 resistance gene. 2. Evaluation of two step protocol for detection of Mi-1 gene. 3. Verification of marker for Ty-1 and evaluation for marker in Guatemala breeding lines. (This is a Geminivirus that is transmitted by the whitefly) 4. Use of molecular markers to detect geminivirus resistance source for Gu143
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