Transformation of E. coli by plasmid DNA 1. Table showing the results from the selective plates |Plate |Plasmid |Contents of plates |Number of colony | | | | |White |Blue | |1 |Ligation mixture |Ampiclillin + X- gal + IPTG |10 |0
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LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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was cut with restriction enzyme Sal I to prepare both for ligation. 3. Vibrio fischeri and pGEM DNA were ligated with T4 ligase. 4. E.coli DH5α competent cells were prepared for us so that transformation of the recombinant plasmids and the competent cells through heat shock transformation. 5. The transformation was then plated on LB/amp/X-gal plates. 6. The plates were screened for our target with ampicillin resistance‚ blue/white colony‚ then bioluminescence. 7. A bioluminescent was selected and
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success? Refer to relevant case studies and example in your discussion. Business operations involve the processes of transformation or more generally‚ production. This involves the transformation of business inputs and business out puts. The business operations may be influenced by the quality expectations‚ technology and globalisation. Business may use the inputs‚ transformation processes and outputs to help them build their business to success. A quality expectation is one of the key goals of
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10.1 HSC topic: Operations -> is about planning & controlling operations with the aim of minimizing cost‚ maximizing productivity‚ improving efficiency and achieving strategic business goals 25% of indicative time The focus of this topic is the strategies for effective operations management in large businesses. Outcomes The student: H1 critically analyses the role of business in Australia and globally H2 evaluates management strategies in response to changes in internal and external
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2/15/2013 background on transformation of bacteria with pGLO plasmid Experiment #5 Aim: Purpose of this lab is to have plasmid activity transformed Material: Bacteria starter plate‚ pGLO DNA Plasmid‚ microcentrifuge tubes‚ Ice‚ water bath‚ CaCl2 Transformation solution‚ (LB) agar plate‚ (LB/Amp) agar plate‚ (LB/Amp/ara) agar plate‚ Micropipette‚ and Micropipette tips. Method: Genetic transformation is a procedure which is done by taking genes from one organism and putting them in another organism
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the world and made the world electricity dependent when compared to the people in the past. ARGUMENT 1: When we observe our present scenario‚ we can see that technology has been the dominant area of knowledge which has lead to the rapid transformation of the world. MAIN EXAMPLE: In the 1900’s people travelled a lot and waited for long hours to buy products and book tickets. They used to move from library to library to gather information for a topic but at present
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An organization creates value by converting inputs into outputs. It takes several resources such as raw materials‚ machinery‚ information‚ knowledge‚ human resources‚ money and capital as inputs and applies appropriate production and transformation processes at the conversion stage to produce finished goods and services. Consumers purchase these goods and services to satisfy their needs. The environment‚ in which an organization operates‚ affects stages in the value-creation process
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Area to Volume on Transformation Efficiency in Escherichia coli K-12 Eliana Pouchard‚ Steve Teng‚ and Cameron Wong Department of Microbiology & Immunology‚ UBC In the process of transformation‚ bact eria take up DNA fr om the environment through their cell wall. To induce competence in the cells‚ the DNA for uptake must first attach to the cell surface prio r to passing throug h the membrane. Previous studies on the effect of surface area on transformation e fficiency have shown
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Honors Essay Three The story of cinderella is most known for the transformation of a poor sweet lady into a princess. In many ways pygmalion is the same as cinderella. The only difference is that pygmalion has three characters undergoing transformations. Eliza could be seen as cinderella‚ because she goes and becomes a young lady in fancy clothes marrying a gentleman. Eliza undergoes the most dramatic transformation due to her having to learn a whole new language. At that point changing
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