From performing the pH experiment it has proven that pH buffer 7 is the optimum pH for catalase in potatoes. This is because the height of the bubbles was the highest of all three tests (pH 3‚ pH 7‚ pH 9) reaching an average height of 0.433 cm. See graph 3. While pH buffer 3’s average height was 0.233 cm only reaching a maximum height of 0.3 cm in Trial 3 and pH buffer 9’s average height being 0.333 cm only reaching a maximum height of 0.4 cm again in Trial 3. See table 1. Therefore this supports
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peroxide and catalase. Tests were performed by putting chicken liver‚ ethanol solution (diluted ethanol solution for other trials) and hydrogen peroxide in a test tube with a side arm‚ and having a rubber tube lead the oxygen gas into a gas collection tube. Results from the tests showed a negative correlation‚ this means that the more diluted the solution of 95% ethanol was‚ the less oxygen gas collected. Controlling a number of factors which include human error‚ temperature change‚ pH levels‚ substrate
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The effect of enzyme concentration‚ substrate concentration‚ pH‚ and temperature on the enzyme catalase. Introduction: Enzymes are biological catalysts; proteins and RNA. They are required for most biological reactions and they are highly specific. Each enzyme has an active site. The active site is the spot on the enzyme where a substrate fits in. Substrates binds with enzymes through the active site. Enzymes‚ being highly specific‚ only fit with one certain substrate. Enzymes and substrates
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Abstract In this laboratory exercise we studied enzyme catalase‚ which accelerates the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase and measure the rate of activity under different conditions. The laboratory was also conducted in association with a second laboratory that measured the effects of an inhibitor on the enzymes. Changes in temperature and pH along with Substrate Concentration and Enzyme Concentration were the conditions tested in the
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Assessment of Catalase Function Lab Introduction The purpose of this lab report was to test and measure the rate of substrate destruction by an enzyme‚ we tested the destruction of hydrogen peroxide by the enzyme catalase. Hydrogen peroxide is a poisonous by product of metabolism that can damage cells if it is not removed. Catalase is an enzyme that speeds up the breakdown of hydrogen peroxide into water and oxygen gas. H2O2 + catalase → H2O + O2 A catalyst is a substance that lowers the
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Enzyme Catalase What Factors Affect Enzyme Activity Michelina Bartolotto Lab Biology 111B February 2‚ 2014 /media/common.studymode/studymode-upload/stm/files/e1b9a3d6adf94ca848b12159c31f11b0.docx INTRODUCTION Enzymes are proteins that function as biological catalysts (Perry‚ Morton 2007). They maintain the body’s stable internal balance‚ and without them life would
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Title: Enzyme Catalysis of Hydrogen Peroxide by Catalase Problem and Objectives: How do different temperatures and different levels of pH affect the reaction rate of the enzymes in chicken liver? Demonstrate the activity of an enzyme in living tissue‚ observe the effects of changes in temperature and pH on the activity of an enzyme‚ perform analyses for the presence of an enzyme in tissues‚ and analyzing relationships between environmental conditions and enzyme activity. Background: Cells produce
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From the graphs‚ it is evident that an increase in both catalase concentration and substrate concentration resulted in a higher rate of reaction or‚ as observed in the kPa graphs‚ a higher volume of O2(g) formed at the end of the 5 minute trial. Interestingly‚ it should also be noted‚ as it was mentioned in the Figure 2‚ that the trend for the 6mL of 3% H2O2(aq) was more of a linear trend than an exponential decay‚ steadily rising until the end of the 5 minute trial. From this‚ it can be inferred
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Abstract The aim of this study was to test the rate of reactivity of the enzyme catalase on hydrogen peroxide while subject to different concentrations of an inhibitor. The hypothesis was that hydrogen peroxide will be broken down by catalase into hydrogen and oxygen‚ where a higher concentration of inhibitor will yield less oxygen‚ resultant of a lower rate of reaction. Crushed potato samples of equal weight were placed in hydrogen peroxide solutions of various temperatures. The results showed
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Pre-Feasibility Study Potato Flakes & Flour 1 TABLE OF CONTENTS Page 1. 2. 3. 4. 5. 6. 7 8. 9. 10. 11. Objective Products Process Global Export Markets Global Import Markets Local Market Target Markets Raw Material Plant & Equipments Conclusion Role of PAMCO 3 3 3 4 5 6 7 8 9 10 10 2 OBJECTIVE: • To provide marketable and value added dehydrated potato products from raw potatoes for the use of food processing industry in Pakistan and abroad as well as end products for the consumer
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