The Comparative Analysis of Simple Staining and Gram Staining Techniques by observing E. Coli and S. Pyogenes under the Compound Light Microscope INTRODUCTION: A German bacteriologist‚ Dr. Theodore von Escherich‚ was the first man in 1885 who discovered the bacterium named Escherichia coli‚ which are gram negative and appears in rod shaped. Most kind of bacteria E. Coli does not cause diseases and some strains indeed are beneficial in helping the process of food breaking down in the intestines
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BIOLOGY NOTES TERM 1 2003 MICROSCOPY Ocular - lens used for magnification (usually x10) Objectives - lenses used for magnification Lower powerx4 Medium powerx10 High powerx40 Course adjustment - focuses image‚ moves focus quickly Fine adjustment - focuses image‚ moves focus slowly Stage - specimens are placed here Condenser - focuses light Iris diaphragm - controls amount of light passing through the specimen TOTAL MAGNIFICATION = OCULAR MAGNIFICATION X OBJECTIVE MAGNIFICATION CLASSIFICATION
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A Basic Understanding of Microscopy Assignment 1 Kaplan University-Microbiology Since microorganisms are not visible to the eye‚ the essential tool in microbiology is the microscope. One of the first to use a microscope to observe microorganisms was Robert Hooke‚ the English biologist who observed algae and fungi in the 1660s. In the 1670s‚ “Anton van Leeuwenhoek‚ a Dutch merchant‚ constructed a number of simple microscopes and observed details of numerous forms of protozoa‚ fungi‚ and bacteria”
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in the field of microscopy was exhibited through the learning of basic techniques in his childhood laboratory in his basement‚ and studying microscopy whenever he had the opportunity‚ a passion that would later become his career. EDUCATION Despite the fact that Skip Palenik was steered away by his professors‚ his devotion to microscopy and his strong determination eventually led to his earning of a Bachelor’s of Science degree‚ and he soon began working in a variety of microscopy labs. FAMILY‚ HOME
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Microbiology and Process Analysis laboratory 25/10/2013 Group 1 Microbiology laboratory Abstract The lab exercises were divided into three different analysis; microscopy‚ soil microbiology and bacterial growth. The main aim of laboratory work with Escherichia coli and soil sample was to introduce students to bacterial growth in pure culture and soil microbial flora. The experiment of bacterial growth in pure culture using optical density
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http://www.who.int/malaria/world_malaria_report_2010/en/index.html 2 Outline • Basics of cell biology literature reading • Imaging (I): light microscopy • Imaging (II): electron microscopy • Basics of the diffusion theory 3 Outline • Basics of cell biology literature reading • Imaging (I): light microscopy • Imaging (II): electron microscopy • Basics of the diffusion theory 4 Why Focus on Literature Reading? • Biology is a scientific discipline undergoing rapid development.
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The wavelength of light that causes excitation of a fluorophore is always longer than the wavelength of light emitted by the same fluorophore. F 10. In a light microscope‚ it is not possible to detect light from a source that is smaller than the resolution limit imposed by the wavelength of visible light. F 11. In light microscopy‚ it is not possible to distinguish two objects that are separated by less than about 0.2μm. T 12.The information about how a protein should fold up is contained in
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Worksheet Fill in the following tables: Type of Microscopy State of the Cell (live‚ dead‚ both) Limit of General steps for resolution sample preparation/ fixation Advantage of Technique Phase contrast light microscope Amplitude contrast microscope Optical tweezers Fluorescence/ Confocal Microscopy Transmission Electron Microscope (TEM) Scanning Electron Microscope (SEM) Scientist Robert Hooke Antonie van Leeuwenhoek Ernst Karl Abbe Fritz Zernike Shimomura‚ Chalfie‚ and Tsien Ernst Ruska Schleiden
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mutations in nerve transmission in Caenorhabditis elegans. C. elegans is a species of nematode that has a simple nervous system and has synaptic vesicles that can be easily tagged with green fluorescent protein (GFP) and viewed under fluorescence microscopy. The experiment was to observe three strains of C. elegans and determine which of the phenotypes observed showed certain mutations and then do a comparison
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spore staining. scanning electron microscopy. Gram staining Negative stain Section: True or False 7. Fluorescent microscopes use an ultraviolet (UV) light source and fluorochromes as stains. True False 8. Older‚ pure cultures of Gram-positive organisms can appear red when Gram stained. True False Section: Matching 9. Which microscope is used to view internal structures of unstained‚ living cells? Scanning electron microscope Dark-field microscopy Transmission
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