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ταηνιπ αλΜεδ
BME 42-620 Engineering Molecular Cell Biology

Lecture 06:
Basics of Cell Biology Literature Reading
Methods of Cell Biology (I): Imaging
Basics of the Diffusion Theory

BME42-620 Lecture 06, September 15, 2011

1

A Case Study: Malaria & Artemisinin y • Malaria is a mosquito-borne infectious disease caused by y parasite Plasmodia.
• 225 million cases each year; 1 illi h million deaths in 2010 (World malaria report).
• 4000 recipes
• 380 extracts from 200 herbs
• Compound 191 artemisinin

http://www.laskerfoundation.org/

Youyou Tu y http://www.who.int/malaria/world_malaria_report_2010/en/index.html

2

Outline
• Basics of cell biology literature reading
• Imaging (I): light microscopy
• Imaging (II): electron microscopy
• Basics of the diffusion theory

3

Outline
• Basics of cell biology literature reading
• Imaging (I): light microscopy
• Imaging (II): electron microscopy
• Basics of the diffusion theory

4

Why Focus on Literature Reading?
• Biology is a scientific discipline undergoing rapid development.
• For in-depth understanding of cell biology, it is essential to read primary research literature.
• The cell biology information that can be covered in our textbook is rather limited. limited • Basic skills for effective reading of cell biology research literature can be learned.

5

An Overview of Cell Biology Literature gy •

Journals
– Examples of general purpose journals
- Science
- Nature
- PNAS

– Examples of specialized journals
- Cell and associated journals
- Journal of Cell Biology
-N t
Nature associated j i t d journals l - Plos journals

•

Commercial versus noncommercial journals
- subscription versus open access

•

Review journals and review articles
6

How to Read Cell Biology Papers (I)
•

It is essential to read and evaluate contemporary cell biology papers critically.
- Why so critical?

•

General guidelines
- Fundamentally it is


References: Press, 1993. • Jonathon Howard, Mechanics of Motor Proteins and the Cytoskeleton, Sinauer Associated, 2001. where k is Boltzmann 's constant and T is absolute temperature (Einstein 1905)
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